A5100780676- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Research Studies
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Lung Cancer Treatments and Mutations
- Acute Myeloid Leukemia Research
- Fibroblast Growth Factor Research
- Angiogenesis and VEGF in Cancer
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Colorectal Cancer Treatments and Studies
- Pancreatic and Hepatic Oncology Research
- Aquaculture Nutrition and Growth
- Cell Image Analysis Techniques
- Dental Anxiety and Anesthesia Techniques
- Neuroendocrine Tumor Research Advances
- Neuroblastoma Research and Treatments
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Ferroptosis and cancer prognosis
- Immune cells in cancer
Dana-Farber Cancer Institute
2019-2025
Hubei University of Medicine
2024-2025
Fudan University
2024-2025
Nanjing University
2025
State Key Laboratory of Pharmaceutical Biotechnology
2025
Fujian Medical University
2025
Southern Medical University
2020-2025
Taihe Hospital
2025
Nanfang Hospital
2025
University of Rochester Medical Center
2024
Abstract Single-cell genomics is essential to chart tumor ecosystems. Although single-cell RNA-Seq (scRNA-Seq) profiles RNA from cells dissociated fresh tumors, single-nucleus (snRNA-Seq) needed profile frozen or hard-to-dissociate tumors. Each requires customization different tissue and types, posing a barrier adoption. Here, we have developed systematic toolbox for profiling clinical samples using scRNA-Seq snRNA-Seq, respectively. We analyzed 216,490 nuclei 40 across 23 specimens spanning...
Immune responses to cancer are highly variable, with mismatch repair-deficient (MMRd) tumors exhibiting more anti-tumor immunity than repair-proficient (MMRp) tumors. To understand the rules governing these varied responses, we transcriptionally profiled 371,223 cells from colorectal and adjacent normal tissues of 28 MMRp 34 MMRd individuals. Analysis 88 cell subsets their 204 associated gene expression programs revealed extensive transcriptional spatial remodeling across discover hubs...
Immune checkpoint blockade (ICB) results in durable disease control a subset of patients with advanced renal cell carcinoma (RCC), but mechanisms driving resistance are poorly understood. We characterize the single-cell transcriptomes cancer and immune cells from metastatic RCC before or after ICB exposure. In responders, subsets cytotoxic T express higher levels co-inhibitory receptors effector molecules. Macrophages treated biopsies shift toward pro-inflammatory states response to an...
Importance The ratio of red blood cell distribution width (RDW) to albumin concentration (RAR) has emerged as a reliable prognostic marker for mortality in patients with various diseases. However, whether RAR is associated the general population remains unknown. Objectives To explore all-cause and cause-specific elucidate their dose-response association. Design, Setting, Participants This population-based prospective cohort study used data from participants 1998-2018 US National Health...
Angiogenesis is increasingly recognized as an important prognosticator associated with the progression of lymphoma and attractive target for novel modalities. We report a previously unrecognized mechanism by which endothelium facilitates growth dissemination interacting circulated T cells suppresses activation CD4(+) cells. Global gene expression profiles microdissected from reactive lymph nodes revealed that cell immunoglobulin mucin domain-containing molecule 3 (Tim-3) was preferentially...
Tissue biology involves an intricate balance between cell-intrinsic processes and interactions cells organized in specific spatial patterns, which can be respectively captured by single-cell profiling methods, such as RNA-seq (scRNA-seq), histology imaging data, Hematoxylin-and-Eosin (H&E) stains. While profiles provide rich molecular information, they challenging to collect routinely do not have resolution. Conversely, histological H&E assays been a cornerstone of tissue pathology for...
SUMMARY Neuroblastoma is a pediatric cancer arising from the developing sympathoadrenal lineage with complex inter- and intra-tumoral heterogeneity. To chart this complexity, we generated comprehensive cell atlas of 55 neuroblastoma patient tumors, collected two institutions, spanning range clinical, genetic, histologic features. Our combines single-cell/nucleus RNA-seq (sc/scRNA-seq), bulk RNA-seq, whole exome sequencing, DNA methylation profiling, spatial transcriptomics, proteomic...
Phthalocyanine (Pc), an electro-redox active moiety, has many attractive properties stemming from its large aromatic system and ability to act as a catalyst in electrochemical reactions, such the CO2 reduction reaction (CO2RR). However, due synthetic challenge related geometric requirements poor solubility (strong aggregation), discrete shape-persistent cages consisting of site-isolated, readily accessible Pc moieties have not been available. Here, we report synthesis Zn- Ni-metallated...
Mammalian sperm show an unusual and heavily compacted genomic packaging state. In addition to its role in organizing the compact hydrodynamic head, it has been proposed that chromatin architecture helps program gene expression early embryo. Scores of genome-wide surveys have reported patterns accessibility, nucleosome localization, histone modification, chromosome folding. Here, we revisit these studies light recent reports obtained from mouse epididymis are contaminated with low levels...
Ammonia, as one of the primary water pollutants in aquaculture, has been shown to induce a wide range ecotoxicological effects on aquatic animals. In order investigate antioxidant and innate immune responses crustaceans disrupted by ammonia, red swamp crayfish (Procambarus clarkii) were exposed 0, 15, 30, 50 mg/L total ammonia nitrogen for 30 d, alterations well immunity studied. The results showed that severity hepatopancreatic injury aggravated increasing levels, which mainly characterized...
Abstract Although metastatic disease is the leading cause of cancer-related deaths, its tumor microenvironment remains poorly characterized due to technical and biospecimen limitations. In this study, we assembled a multi-modal spatial cellular map 67 biopsies from 60 patients with breast cancer across diverse clinicopathological features nine anatomic sites detailed clinical annotations. We combined single-cell or single-nucleus RNA sequencing for all panel four expression assays...
Neuroendocrine tumors (NETs) are rare cancers that most often arise in the gastrointestinal tract and pancreas. The fundamental mechanisms driving gastroenteropancreatic (GEP)-NET growth remain incompletely elucidated; however, heterogeneous clinical behavior of GEP-NETs suggests both cellular lineage dynamics tumor microenvironment influence pathophysiology. Here, we investigated single-cell transcriptomes immune cells from patients with NETs. Malignant GEP-NET expressed genes regulons...
Heterogeneous immunoassay based on magnetic separation is commonly used in inductively coupled plasma-mass spectrometry (ICP-MS)-based biomedical analysis with elemental labeling. However, the functionalized beads (MBs) often suffer from non-specific adsorption and random distribution of functional probes. To overcome these problems, DNA tetrahedron (DT)-functionalized MBs were designed further conjugated substrate modified Au NPs (Sub-AuNP). Based prepared MB-DT-AuNP probes, an MB-DT...
Hepatocellular carcinoma (HCC) has a high incidence and poor prognosis. Cuproptosis is novel type of cell death, which differs from previously reported types death such as apoptosis, autophagy, proptosis, ferroptosis, necroptosis, etc. Long non-coding RNAs (lncRNAs) play multiple roles in HCC.
Purpose: Nutritional status is associated with the prognosis of esophageal cancer (EC) patients, which can influence treatment efficacy. Additionally, efficacy consolidation chemotherapy (CCT) after definitive chemoradiotherapy (DCRT) unclear. This study aimed to explore prognostic value nutritional index (PNI) at different periods, as well its on CCT Methods: We reviewed data 106 patients cT2-4N0-3M0 EC who received DCRT between December 2016 and October 2020. Survival analyses were...
Non-small cell lung cancer patients with EGFR mutation have a high rate of brain metastases, and tyrosine kinase inhibitors (TKIs) are the principal therapeutic approach. However, acquired targeted therapy resistance is main reason for EGFR-TKIs' treatment failure. At present, mechanism intracranial limited, mainly due to lack line that can be used its study. A parenchymal metastatic sample progressed after third-generation EGFR-TKI was establish named AlmoR1. The genetic characteristics...
Abstract Insufficient liver regeneration increases the risk of postoperative failure following transplantation or partial hepatectomy (PHx). Numerous growth factors and cytokines are related to regeneration; however, underlying mechanisms have not been fully elucidated. In this study, CXCL13 was identified as a key factor delaying after PHx. We observed that expression upregulated in PHx mice patients resection. deficiency accelerated regeneration, whereas these effects were abolished by...