A5105828401- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Plant Genetic and Mutation Studies
- Wheat and Barley Genetics and Pathology
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Bioenergy crop production and management
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Spectroscopy and Chemometric Analyses
- Plant nutrient uptake and metabolism
- DNA Repair Mechanisms
- Genetically Modified Organisms Research
- Agriculture and Rural Development Research
- Agronomic Practices and Intercropping Systems
- Rice Cultivation and Yield Improvement
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Seed and Plant Biochemistry
- Animal Genetics and Reproduction
- Plant Pathogens and Resistance
- Enzyme Production and Characterization
- Plant Reproductive Biology
- Plant Micronutrient Interactions and Effects
Carlsberg Laboratory
1993-2024
Swedish University of Agricultural Sciences
2023
The Land Institute
2023
The 431 bp C‐hordein promoter of λ‐ 1 ‐ 17 exhibits a specific response to amino acids and NH 4 NO 3 in developing barley ( Hordeum vulgare L.) endosperms. With the aid particle bombardment it is shown that GCN4 motif ATGA(C/G)TCAT dominating cis ‐acting element this response. But synergistic interaction with neighbouring endosperm TGTAAAGT within bifactorial prolamin cooperation upstream sequences including second prolamin‐like an absolute requirement for strong, positive regulation by...
Improved agricultural and industrial production organisms are required to meet the future global food demands minimize effects of climate change. A new resource for crop microbe improvement, designated FIND-IT (Fast Identification Nucleotide variants by droplet DigITal PCR), provides ultrafast identification isolation predetermined, targeted genetic in a screening cycle less than 10 days. Using large-scale sample pooling combination with digital PCR (ddPCR) greatly increases size...
Sustainable agriculture in the future will depend on crops that are tolerant to biotic and abiotic stresses, require minimal input of water nutrients can be cultivated with a carbon footprint. Wild plants fulfill these requirements abound nature but typically low yielding. Thus, replacing current high-yielding less productive resilient species intractable trade-off increasing land area under cultivation produce same yield. Cultivating more reduces natural resources, biodiversity increases...
In nature, genetic variation occurs in every population and results the evolution of a diversity new properties, some which promote survival species. To accelerate nature's based on diversity, plant breeders may induce additional mutations to raise number variations increasing chances obtain varieties with desired traits like improved nutritive quality, yields resilience biotic abiotic stress factors. Induced mutagenesis chemical mutagens is considered non-GM has been used barley (Hordeum...
Plant breeders leverage mutagenesis using chemical, biological, and physical mutagens to create novel trait variations. Many widely used sorghum genotypes have a narrow genetic base, which hinders improvements classical breeding. Enhancing the diversity of genome thus remains key priority for breeders. To accelerate enhancement sorghum, an extensive library comprised seeds from 150,000 individual mutant plants Sorghum bicolor inbred line BTx623 was established ethyl methanesulphonate (EMS)...
<title>Abstract</title> Chemical, biological, and physical mutagens induce modifications of nucleotides in the exposed organisms, resulting base changes their DNA. When harnessed, mutagenesis increases genetic diversity crops aids elucidating gene function through study mutants with altered phenotypes, particularly when combined reverse techniques such as Fast Identification Nucleotide variants by droplet digital PCR (FIND-IT). Sorghum (<italic>Sorghum bicolor</italic>) is a prime candidate...
Chitinases are expressed in various plant tissues where they thought to play a role defense against chitin-containing pathogens. Transient gene expression assays have been used of barley delineate promoter sequences involved the regulation an aleurone-specific chitinase (Chi26), and vegetatively (Chi33). The measured activities transcriptional fusions between 5' upstream GUS reporter genes after DNA delivery by particle bombardment. Analysis Chi26 3' deletions indicated that -200 -140 confer...
Application of the mlo-based resistance in barley against powdery mildew attacks is a major success crop breeding, since it confers durable disease resistance. Resistance caused by mutations Mlo gene seems to be ubiquitous across range species. This work addresses introduction into hexaploid wheat, which complicated occurrence three homoeologous genes: Mlo-A1, Mlo-B1 and Mlo-D1. EMS-generated mutant plants were screened for homoeologues. We selected combined 6, 8, 4 mutations, respectively,...
Perennial grains would reduce the environmental impact of food production.Perennial can improve soil health and sequester atmospheric carbon