A5020997600- PI3K/AKT/mTOR signaling in cancer
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Cytokine Signaling Pathways and Interactions
- Protein Kinase Regulation and GTPase Signaling
- Immune cells in cancer
- Animal Virus Infections Studies
- Growth Hormone and Insulin-like Growth Factors
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Metabolism, Diabetes, and Cancer
- Cancer-related molecular mechanisms research
- Cancer Immunotherapy and Biomarkers
- Virus-based gene therapy research
- Cancer Research and Treatments
- Plant Virus Research Studies
- RNA Research and Splicing
- Peptidase Inhibition and Analysis
- Cancer, Lipids, and Metabolism
- Ferroptosis and cancer prognosis
- Cancer-related gene regulation
- Histone Deacetylase Inhibitors Research
- Cancer Mechanisms and Therapy
- Proteoglycans and glycosaminoglycans research
- Diet and metabolism studies
China Medical University
2013-2024
National Health Research Institutes
2009-2024
National Tsing Hua University
2013-2023
Icahn School of Medicine at Mount Sinai
2003-2023
Beijing Institute of Petrochemical Technology
2021-2022
University of California, Berkeley
1973-2015
Rockefeller University
1982-2015
Chi Mei Medical Center
2012-2013
Chang Gung University
2013
China Medical University Hospital
2013
Metastasis, the cardinal feature of malignant tumors, is an important clinical variable in patient prognosis. To understand basis for metastasis, we systematically selected highly invasive cells from breast cancer cell lines, MCF7 and MDA-MB-453, with moderate to low ability using Boyden chamber invasion assay. The four-cycle named MCF7-I4 MDA-MB-453-I4, respectively, displayed epithelial-mesenchymal transition (EMT) dramatically enhanced ability. EMT changes were corroborated decreased...
The early onset breast cancer patients (age ≤ 40) often display higher incidence of axillary lymph node metastasis, and poorer five-year survival than the late-onset patients. To identify genes molecules associated with poor prognosis cancer, we examined gene expression profiles from paired normal/tumor tissues, coupled Gene Ontology public data base analysis. Our showed that GAS7b was lower in as compared to elder We found GAS7 CYFIP1 WAVE2 complex suppress metastasis via blocking Rac1...
To explore the basis of metastasis, we compared human breast cancer lines MCF-7 and MDA-MB453, which have low invasive ability, with their sublines MCF7-I4 MDA-MB453-I4 high ability for gene expression signaling pathways. We previously showed that I4 had dramatically elevated levels Twist parental lines. In this study, observed significantly increased STAT3 Tyr(705) phosphorylation, but not protein levels, in Activation by interleukin-6 or activated Src induced at mRNA levels. Inhibiting a...
Metastasis is the major factor affecting patient survival in ovarian cancer. However, its molecular mechanisms remain unclear. Our study used isogenic pairs of low‐ and high‐invasive cancer cell lines to demonstrate downregulation miRNA‐138 highly invasive cells, functioning as an inhibitor migration invasion. An orthotopic xenograft mouse model further demonstrated that expression inhibited metastasis other organs. Results indicated miR‐138 directly targeted SRY‐related high mobility group...
AbstractThe insulin-like growth factor I (IGF-I) receptor (IGF-IR) is known to regulate a variety of cellular processes including cell proliferation, survival, differentiation, and transformation. IRS-1 Shc, substrates the IGF-IR, are mediate IGF-IR signaling pathways such as those mitogen-activated protein kinase (MAPK) phosphatidylinositol 3-kinase (PI3K), which believed play important roles in some IGF-IR-dependent biological functions. We used cytoplasmic domain yeast two-hybrid...
Abstract MicroRNAs offer tools to identify and treat invasive cancers. Using highly isogenic oral squamous cell carcinoma (OSCC) cells, established using in vitro vivo selection protocols from poorly parental populations, we used microarray expression analysis a relative specific decrease miR-491-5p cells. Lower of correlated with poor overall survival patients OSCCs. overexpression OSCC cells suppressed their migratory behavior lung metastatic vivo. We defined the G-protein—coupled receptor...
AKT (also known as PKB) plays a central role in variety of cellular processes including cell growth, motility and survival both normal tumor cells. The pathway is also instrumental epithelial mesenchymal transitions (EMT) angiogenesis during tumorigenesis. functions cardinal nodal point for transducing extracellular (growth factors insulin, IGF-1 EGF ) intracellular (such mutated/activated receptor tyrosine kinases, PTEN, Ras Src) signals. It positively regulated by phosphatidylinositol...
Glucocorticoids are widely used in conjunction with chemotherapy for ovarian cancer to prevent hypersensitivity reactions. Here we reveal a novel role glucocorticoids the inhibition of metastasis. Glucocorticoid treatments induce expression miR-708, leading suppression Rap1B, which result reduction integrin-mediated focal adhesion formation, cell migration/invasion and impaired abdominal metastasis an orthotopic xenograft mouse model. Restoring Rap1B reverts glucocorticoid-miR-708...
Targeting the c-Met signaling pathway has become a therapeutic strategy in multiple types of cancer. We unveiled novel regulating mechanism that could be applied as modality for oral squamous cell carcinoma (OSCC) therapy. Upregulation keratin 16 (KRT16) was found by comparing isogenic pairs low and high invasive human OSCC lines via microarray analysis. cells with ectopic expression or silencing KRT16 were used to scrutinize functional roles associated molecular mechanisms. observed...
Article29 August 2019Open Access Source DataTransparent process Dysregulation of cystathionine γ-lyase promotes prostate cancer progression and metastasis Yi-Hsiang Wang Institute Molecular Genomic Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan College Life Science, Tsing Hua University, Hsinchu, Search for more papers by this author Jo-Ting Huang Wen-Ling Chen Rong-Hsuan Biotechnology, Ming-Chien Kao Yan-Ru Pan Shih-Hsuan Chan Chiese Medicine Center, Integrated...
Abstract Background Ovarian cancer has the highest mortality among gynecological cancers due to late diagnosis and lack of effective targeted therapy. Although study interplay between cells with their microenvironment is emerging, how ovarian triggers signaling that coordinates immune promote metastasis still elusive. Methods Microarray bioinformatics analysis low highly invasive cell lines were used reveal periostin (POSTN), a matrix protein multifunctions in cancer, elevated expression...
The development of drug resistance in oral squamous cell carcinoma (OSCC) that frequently leads to recurrence and metastasis after initial treatment remains an unresolved challenge. Presence cancer stem cells (CSCs) has been increasingly reported be a critical contributing factor resistance, tumor metastasis. Thus, unveiling mechanisms regulating CSCs potential targets for developing their inhibitors will instrumental improving OSCC therapy.siRNA, shRNA miRNA specifically target keratin 17...
Rationale: Multiple copies in T-cell malignancy 1 (MCT-1) is a prognostic biomarker for aggressive breast cancers.Overexpressed MCT-1 stimulates the IL-6/IL-6R/gp130/STAT3 axis, which promotes epithelial-to-mesenchymal transition and cancer stemness.Because stemness largely contributes to tumor metastasis recurrence, we aimed identify whether blockade of IL-6R can render these effects understand underlying mechanisms that govern process.Methods: We assessed primary invasion, postsurgical...
A recently reported new member of the Vav family proteins, Vav3 has been identified as a Ros receptor protein tyrosine kinase (RPTK) interacting by yeast two-hybrid screening.Northern analysis shows that broad tissue expression profile is distinct from those and Vav2.Two species transcripts, 3.4 5.4 kb, were detected with differential pattern in various tissues.Transient 293T NIH 3T3 cells demonstrated ligand stimulation several RPTKs (epidermal growth factor [EGFR], Ros, insulin [IR],...
We isolated a human gene (designated c-ros-1) homologous to the v-ros sequence of UR2 sarcoma virus. Ten exons, 1,414 base pairs spanning 26 kilobases, contained tyrosine kinase domain, transmembrane and part an extracellular domain carrying N glycosylation site which was not acquired by The predicted structure c-ros-1 is unique among src family clearly distinct from insulin receptor.
Abstract Purpose: SOX9 is an important transcription factor required for development and has been implicated in several types of cancer. However, never linked to lung cancer date. Here, we show that expression upregulated adenocarcinoma how it associated with cell growth. Experimental Design: Data mining five microarray data sets containing 490 clinical samples, quantitative reverse transcription-PCR validation assay 57 independent immunohistochemistry tissue microarrays 170 cores were used...
Current understanding of the activation STATs is through binding between SH2 domain and phosphotyrosine tyrosine kinases.Here we demonstrate a novel role RACK1 as an adaptor for insulin insulin-like growth factor 1 receptor (IGF-1R)-mediated STAT3 specifically.Intracellular association via its N-terminal WD domains to 4 (WD1-4) with (IR)/IGF-1R augmented upon respective ligand stimulation, whereas constitutive.Purified or WD1-4 associates directly purified IR, IGF-1R, in vitro.Insulin...
AKT (also known as PKB) plays a central role in variety of cellular processes including cell growth, motility and survival both normal tumor cells. The pathway is also instrumental epithelial mesenchymal transitions (EMT) angiogenesis during tumorigenesis. functions cardinal nodal point for transducing extracellular (growth factors insulin, IGF-1 EGF ) intracellular (such mutated/activated receptor tyrosine kinases, PTEN, Ras Src) signals. It positively regulated by phosphatidylinositol...
The poly(A) sequence of 30 to 40S Rous sarcoma virus RNA, prepared by digestion the RNA with RNase T(1), showed a rather homogenous electrophoretic distribution in formamide-polyacrylamide gels. Its size was estimated be about 200 AMP residues. appears located at or near 3' end because: (i) it contained one adenosine per 180 residues, and because (ii) incubation bacterial H presence poly(dT) removed its without significantly affecting hydrodynamic properties denaturing solvents. viral 60 70S...
Increased Crabp2 levels have been found in various types of cancer, and are associated with poor patients' survival. Although is to be overexpressed lung its role metastasis cancer unclear. In this study, was high-metastatic C10F4 than low-metastatic cells. Analysis clinical samples revealed that high CRABP2 were correlated lymph node metastases, overall survival, increased recurrence. Knockdown decreased migration, invasion, anoikis resistance, vivo metastasis. co-immunoprecipitated HuR,...