A5105930794- PI3K/AKT/mTOR signaling in cancer
- Cancer Mechanisms and Therapy
- Tuberous Sclerosis Complex Research
- RNA modifications and cancer
- Mast cells and histamine
- Concrete and Cement Materials Research
- Synthesis and biological activity
- Innovative concrete reinforcement materials
- Hippo pathway signaling and YAP/TAZ
- Magnesium Oxide Properties and Applications
- Melanoma and MAPK Pathways
- Protein Kinase Regulation and GTPase Signaling
- Hedgehog Signaling Pathway Studies
- MXene and MAX Phase Materials
- Multiple Sclerosis Research Studies
- Neuroscience and Neuropharmacology Research
- Tryptophan and brain disorders
- Autoimmune Bullous Skin Diseases
- Fire effects on concrete materials
- Stress Responses and Cortisol
- Advanced Welding Techniques Analysis
- Grouting, Rheology, and Soil Mechanics
- Aluminum Alloys Composites Properties
- Microtubule and mitosis dynamics
- Cancer Immunotherapy and Biomarkers
The Seventh Affiliated Hospital of Sun Yat-sen University
2025
Sun Yat-sen University
2025
Army Medical University
2025
Anhui University of Science and Technology
2015-2024
Shanxi Medical University
2023
Second Hospital of Shanxi Medical University
2023
Dana-Farber Cancer Institute
2012-2023
Florida College
2007-2023
University of South Florida
2007-2023
Molecular Oncology (United States)
2007-2023
Metastasis, the cardinal feature of malignant tumors, is an important clinical variable in patient prognosis. To understand basis for metastasis, we systematically selected highly invasive cells from breast cancer cell lines, MCF7 and MDA-MB-453, with moderate to low ability using Boyden chamber invasion assay. The four-cycle named MCF7-I4 MDA-MB-453-I4, respectively, displayed epithelial-mesenchymal transition (EMT) dramatically enhanced ability. EMT changes were corroborated decreased...
Class Ia phosphatidylinositol 3 kinase (PI3K) is required for oncogenic receptor-mediated transformation; however, the individual roles of two commonly expressed class PI3K isoforms in receptor signaling have not been elucidated vivo. Here, we show that genetic ablation p110α blocks tumor formation both polyoma middle T antigen (MT) and HER2/Neu transgenic models breast cancer. Surprisingly, p110β results increased ductal branching tumorigenesis. Biochemical analyses suggest a competition...
Reproducing the pathophysiology of human multiple sclerosis (MS) in animal models is critical to identifying mechanisms triggering demyelination and developing early intervention strategies. Here, we aimed model overactivated Wnt (wingless-related integration site) signaling previously shown postmortem brain tissues patients with MS by inducing experimental autoimmune encephalomyelitis (EAE) Pdgfra CreER ;Apc fl/fl Olig2 Cre mice. These mice have oligodendrocyte precursor cells (OPCs)...
Significance Aberrant activation of the PI3K pathway is a frequent event in human cancer, making an attractive target cancer therapy. Early generation inhibitors have poor efficacy and intolerable side effects; new isoform-selective are emerging clinic. Much work ongoing to determine isoform dependence different cancers. Of ubiquitously expressed isoforms, p110α critical for activated receptor tyrosine kinases or oncogenes, whereas p110β seems essential many tumors deficient phosphatase...
// Heba Alshaker 1, 2 , Qi Wang 1 Yoshiaki Kawano 3 Tawfiq Arafat 4 Torsten Böhler 5 Mathias Winkler Colin Cooper Dmitri Pchejetski School of Medicine, University East Anglia, Norwich, UK Department Pharmacology and Biomedical Sciences, Faculty Pharmacy Medical Petra, Amman, Jordan Urology, Kumamoto, Japan Pharmaceutical Medicinal Chemistry Pharmacognosy, Surgery Cancer, Imperial College London, Correspondence to: Alshaker, email: halshaker@uop.edu.jo Pchejetski, d.pshezhetskiy@uea.ac.uk...
PTEN hamartoma tumor syndrome (PHTS) comprises a collection of genetic disorders associated with germline mutations in the suppressor gene . Therapeutic options and preventative measures for PHTS are limited. Using both genetically engineered mouse models pharmacological PI3K isoform-selective inhibitors, we found that roles isoforms spatially distinct skin: While p110α is responsible sustained survival suprabasal cells epidermis absence PTEN, p110β important hyperproliferation basal PHTS....
In this work, an improved explosive welding technique was investigated to fabricate a thin Mg/Al plate, where additional aluminum sheet used as buffer layer between the and Al Mg plate rigidly constrained by steel avoid excessive deformation. Moreover, parameters were optimized using theoretical analysis numerical simulation, interfacial behavior simulated SPH method. The bonding properties of achieved joints microstructure observation mechanical tests. It concluded that is effective method...
Radiation is an important therapeutic strategy for hepatocellular (HCC). In this study, we evaluated the role of dual PI3K/mTOR inhibitor, PKI-587, on radiosensitization HCC and its possible mechanism. MTT, colony formation, flow cytometry, immunofluorescence were used to analyze proliferation, cell cycle, formation residual γ-H2AX foci, apoptosis cells. A SK-Hep1 xenograft model was assess effects PKI-587 in combination with ionizing radiation vivo. The activation levels PI3K/AKT/mTOR DNA...
Abstract To study the effect of internal particle size on microstructure properties and thermal decomposition characteristics site mixed emulsion explosive at different altitudes. Site was prepared with shear rate. The size, viscosity, sensitized bubbles, detonation velocity peak pressure were tested after stored simulated matrix three rotational speeds measured by thermogravimetric analyzer kinetic analysis performed non-isothermal model Kissinger–Akah–Sunose (KAS) method. results show that...
Aiming at the support difficulties of mine galleries under complex geological conditions high buried depth, in situ stress and tectonic fractured zones, a novel square-steel-confined-concrete (SSCC) arch centering supporting system is proposed this paper. To validate feasibility system, uniaxial compression test on an SSCC short column performed results illustrate that it has better ductility higher bearing capacity than U-shaped column. Numerical analysis mechanical behaviors conducted...
In deep coal mining, grouting reinforcement and water blockage are the most effective means for reinforcing rock mass of extremely broken coal. However, traditional cement materials not suitable use in complex strata because their insufficient early mechanical strength slow setting time. This study innovatively proposes using alkali-activated material to compensate shortcomings strengthen unstable mass. The was prepared slag as raw combined with sodium hydroxide liquid silicate activation....
Abstract Tobacco use is a major challenge to public health in the United States and across world. Many studies have demonstrated that adult men women differ their responses tobacco smoking, however neurobiological about effect of smoking on males females were limited. Exchange protein directly activated by cAMP (Epac) signaling participates drug addictive behaviors. In this study, we examined hippocampal Epac nicotine-induced place conditioning mice. Nicotine at 0.2 mg/kg 0.4 induced...
Nicotine withdrawal symptoms, mainly anxiety, cause high level of relapse rate after quitting smoking. Vitamin D supplementation has shown its potential for the prevention and treatment anxiety disorders; however, neurobiological studies about effect vitamin on nicotine withdrawal-induced are limited. To investigate molecular mechanism D3 supplement by dietary anxiety-like behavior during withdrawal, male C57/BL6 mice were divided into four groups: vehicle, only, plus D3. Mice administrated...
To resolve problems caused by the accumulation of flue gas desulfurization gypsum (FGDG) in environment, a polymer material was prepared using FGDG, granulated blast furnace slag (GBFS), fly ash (FA), and solid sodium silicate (SSS). The compressive strength these specimens cured for 3, 28, 60 d regularly measured, their condensation behavior analyzed. Both formation mineral crystals microstructure characteristics were analyzed further X-ray diffraction scanning electron microscopy. pure...
The PTEN hamartoma tumor syndrome (PHTS) is a complex disorder caused by germline inactivating mutations of the suppressor gene PTEN. Loss function leads to unimpeded phosphatidylinositol-3′-kinase (PI3K) activity and PI3K-driven cell division. Individuals with PHTS develop benign hamartomas in various tissues have an increased risk developing malignant diseases. Notably, no effective therapy currently exists for this disorder. Using both genetic mouse models pharmacological approaches, we...
To further enhance the compressive strength of alkali-activated materials and reveal their condensation behavior, reactivity slag was enhanced through addition different kinds proportions sodium silicate. The mechanical properties specimens were observed regularly behavior analyzed. results showed that both solid liquid silicate could significantly improve strength. maximum increase in 123.7%, while initial final setting times shortened to 9 min. When content increased from 5% 15%, first...
Abstract Osimertinib sensitive and resistant NSCLC NCI-H1975 clones were used to model osimertinib acquired resistance in humanized mice delineate potential mechanisms. No new EGFR mutations or loss of the T790M mutation found clones. Resistant tumors initially partially responsive osimertinib, then aggressive tumor regrowth occurred accompanied by an immunosuppressive microenvironment. 3-phosphoinositide-dependent kinase 1 (PDK1) was identified as a driver resistance, its selective...
Abstract Background Observational studies have reported an association between circulating levels of mammalian target rapamycin (mTOR)-dependent proteins and multiple sclerosis (MS). However, the casual has not been fully elucidated. Mendelian randomization (MR) is used to overcome limitations inherent observational assess causal association. Methods To explore mTOR-dependent (AKT, RP-S6K, eIF4E-BP, eIF4A, eIF4E, eIF4G, PKC-α) MS, summary statistics were obtained from GWAS meta-analysis...
<div>Abstract<p>Metastasis, the cardinal feature of malignant tumors, is an important clinical variable in patient prognosis. To understand basis for metastasis, we systematically selected highly invasive cells from breast cancer cell lines, MCF7 and MDA-MB-453, with moderate to low ability using Boyden chamber invasion assay. The four-cycle named MCF7-I4 MDA-MB-453-I4, respectively, displayed epithelial-mesenchymal transition (EMT) dramatically enhanced ability. EMT changes were...
<div>Abstract<p>Metastasis, the cardinal feature of malignant tumors, is an important clinical variable in patient prognosis. To understand basis for metastasis, we systematically selected highly invasive cells from breast cancer cell lines, MCF7 and MDA-MB-453, with moderate to low ability using Boyden chamber invasion assay. The four-cycle named MCF7-I4 MDA-MB-453-I4, respectively, displayed epithelial-mesenchymal transition (EMT) dramatically enhanced ability. EMT changes were...