A5031722158- Enzyme Production and Characterization
- Advanced Proteomics Techniques and Applications
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Ruminant Nutrition and Digestive Physiology
- Photosynthetic Processes and Mechanisms
- Microbial Community Ecology and Physiology
- Biochemical and Structural Characterization
- Crustacean biology and ecology
- Aquaculture Nutrition and Growth
- Protein Structure and Dynamics
- Bacterial biofilms and quorum sensing
- Meat and Animal Product Quality
- Glycosylation and Glycoproteins Research
- Enzyme Catalysis and Immobilization
- Fish biology, ecology, and behavior
- Animal health and immunology
- Mass Spectrometry Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Fuel Cells and Bioremediation
- Antimicrobial Peptides and Activities
- Protein Hydrolysis and Bioactive Peptides
- Venomous Animal Envenomation and Studies
- Microbial Metabolic Engineering and Bioproduction
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2025
National University of Mar del Plata
2012-2025
Fundación Ciencias Exactas y Naturales
1995-2024
Abstract While many aspects of archaeal cell biology remain relatively unexplored, systems approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount MS data generated often remains incompletely analyzed due to a lack sophisticated bioinformatic tools and field-specific biological expertise interpretation. Here we present initiation Archaeal Proteome Project (ArcPP), community-based effort comprehensively analyze...
Bacteria communicate at high cell density through quorum sensing, however, there are no reports about this mechanism in archaea. The archaeon Natronococcus occultus produces an extracellular protease the end of growth. Early production activity was observed when a low culture incubated with late exponential conditioned medium suggesting presence factor(s) inducing activity. Conditioned and ethyl acetate extracts corresponding to transition from stationary phase showed positive signal...
Abstract Background Natrialba magadii is an aerobic chemoorganotrophic member of the Euryarchaeota and a dual extremophile requiring alkaline conditions hypersalinity for optimal growth. The genome sequence Nab. type strain ATCC 43099 was deciphered to obtain comprehensive insight into genetic content this haloarchaeon understand basis some cellular functions necessary its survival. Results consists four replicons with total 4,443,643 bp encodes 4,212 putative proteins, which contain peptide...
The membrane protease LonB is an essential protein in the archaeon Haloferax volcanii and globally impacts its physiology. However, natural substrates of archaeal Lon have not been identified. whole proteome turnover was examined a H. mutant under reduced physiological levels. LC–MS/MS combined with stable isotope labeling applied for identification/quantitation cytoplasm proteins. Differential synthesis degradation rates were evidenced 414 proteins response to expression. A total 58...
Rhomboid proteases are intramembrane serine that play a key role in regulating membrane proteins across all domains of life. However, their function archaea remains poorly understood. The model halophilic archaeon Haloferax volcanii encodes two rhomboid homologs, rho1 (HVO_1474) and rho2 (HVO_0727). Previous studies indicated the deletion resulted mild alterations motility, adhesion, biofilm formation, cell morphology, suggesting potential functional compensation by rho1. To investigate...
Rhomboid proteases occur in all domains of life; however, their physiological role is not completely understood, and nothing known the biology these enzymes Archaea. One two rhomboid homologs Haloferax volcanii (RhoII) fused to a zinc finger domain. Chromosomal deletion rhoII was successful, indicating that this gene essential for organism; mutant strain (MIG1) showed reduced motility increased sensitivity novobiocin. Membrane preparations MIG1 were enriched glycoproteins, identified as...
Although homologs of the ATP-dependent Lon protease exist in all domains life, relevance this archaeal physiology remains a mystery. In study, we have constructed and phenotypically characterized deletion conditional lon mutants model haloarchaeon Haloferax volcanii to elucidate role unusual membrane-bound LonB archaea. Hvlon could be deleted from chromosome only when copy wild type gene was provided trans suggesting that is essential for survival archaeon. Successful complementation lethal...
The effects of acetic, propionic, butyric and lactic acids (short-chain acids), glycerol, on the in vitro dry matter digestibility (IVDMD) oat hay, cellulolysis by a protein extract rumen fluid were studied. objective was to gain information impact increasing concentrations those compounds medium degradative activities carried out microorganisms. Short-chain assayed as sodium salts and, for all compounds, 50, 100, 200 300 mM. volatile fatty (VFA: propionic acids) tested separately or two...
ABSTRACT Halolysins are subtilisin-like extracellular proteases produced by haloarchaea that possess unique protein domains and salt dependent for structural integrity functionality. In contrast to bacterial subtilases, the maturation mechanism of halolysins has not been addressed. The halolysin Nep is secreted alkaliphilic haloarchaeon Natrialba magadii , recombinant active enzyme synthesized in Haloferax volcanii . contains an N-terminal signal peptide with typical Tat consensus motif (G...
Aims: The alkaliphilic haloarchaeon Natrialba magadii secretes a halolysin‐like protease (Nep) that is active and stable in high salt organic solvents, which represents potential resource for biocatalysis low water activity conditions. In this study, the effect of growth stage on Nep biosynthesis was examined. Methods Results: mRNA extracellular were measured by RT‐PCR azocaseinolytic determination, respectively. Increased abundance observed Nab. cells with culture age, correlated...
Rhomboids are conserved intramembrane serine proteases involved in cell signaling processes. Their role prokaryotes is scarcely known and remains to be investigated Archaea. We previously constructed a rhomboid homologue deletion mutant (ΔrhoII) Haloferax volcanii, which showed reduced motility, increased novobiocin sensitivity, an N- glycosylation defect. To address the impact of rhoII on H. volcanii physiology, proteomes parental strains were compared by shotgun proteomics. A total 1847...
The effect of various nitrogen sources and nutritional starvation was examined on the production an extracellular protease secreted by haloalkaliphilic archaeon Natrialba magadii.Cell growth proteolytic activity were measured in cells grown with different sources. Proteolytic produced complex easily metabolized such as yeast extract, casein casamino acids; meanwhile, ammonium repressed enzyme production. time course amount accumulated showed inverse correlation rate nutrient concentration....
Abstract The dynamic changes that take place along the phases of microbial growth (lag, exponential, stationary, and death) have been widely studied in bacteria at molecular cellular levels, but little is known for archaea. In this study, a high‐throughput approach was used to analyze compare proteomes two haloarchaea during exponential stationary growth: neutrophilic Haloferax volcanii alkaliphilic Natrialba magadii . Almost 2000 proteins were identified each species (≈50% predicted...
In spite of its inherent physiological importance, studies on the occurrence, characteristics, and modulation amylase in euryhaline crabs are lacking. We investigated occurrence forms effect acclimation to different salinities their number made a partial purification characterization major form present hepatopancreas Neohelice granulata. Zymogram analysis revealed 5 acclimated 35 psu (seawater) 37 psu, an additional band at 10 but with (29 kDa) all cases, which was partially purified...
Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon Natrialba magadii. Many proteases have been characterized from archaea to bacteria as adapted hypersaline environments retaining function and stability until 4.0M NaCl. As observed in other halolysins, this can be related presence of C-terminal extension (CTE) sequence. In present work, we compared biochemical properties recombinant protease with truncated form at 134 amino...
Phytoene synthase (PSY) converts two molecules of geranyl-geranyl diphosphate to phytoene, the key regulatory step in carotenogenesis. However, post-translational mechanisms that control PSY expression are scarcely understood. Carotenoid biosynthesis (mainly bacterioruberin) is a distinctive feature haloarchaea thriving hypersaline environments. Carotenogenesis negatively regulated by AAA+ LonB protease haloarchaeon Haloferax volcanii as it controls degradation. We investigated relevance...