A5078846994- Antifungal resistance and susceptibility
- Microbial Natural Products and Biosynthesis
- Trace Elements in Health
- Plant-Microbe Interactions and Immunity
- Chromium effects and bioremediation
- Indoor Air Quality and Microbial Exposure
- Fungal Biology and Applications
- Advanced Chemical Sensor Technologies
- Medicinal Plants and Neuroprotection
- Plant Pathogens and Fungal Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Canadian Identity and History
- Fungal and yeast genetics research
- Arctic and Russian Policy Studies
- Heavy Metal Exposure and Toxicity
- Ocular Infections and Treatments
- Mycotoxins in Agriculture and Food
- Vitamin C and Antioxidants Research
- Occupational exposure and asthma
- Genomics and Phylogenetic Studies
University of Wisconsin–Madison
2018-2021
Aspergillus fumigatus is a ubiquitous saprophytic mold able to grow on diversity of material ranging from decayed organic matter in the environment space station cupolas [1]. Yet this fungus equally adept as serious opportunistic pathogen, causing pulmonary aspergillosis and more deadly invasive (IA). There are an estimated 3,000,000 cases annually than 200,000 IA each year reaching mortality rate up 90% most susceptible populations [2]. Difficulties treating include delayed detection...
In immunocompromised individuals, Aspergillus fumigatus causes invasive fungal disease that is often difficult to treat. Exactly how immune mechanisms control A. in immunocompetent individuals remains unclear. Here, we use transparent zebrafish larvae visualize and quantify neutrophil macrophage behaviors response different strains. We find macrophages form dense clusters around spores, establishing a protective niche for survival. Macrophages exert these effects by inhibiting germination,...
ABSTRACT Microbial secondary metabolites, including isocyanide moieties, have been extensively mined for their repertoire of bioactive properties. Although the first naturally occurring (xanthocillin) was isolated from fungus Penicillium notatum over half a century ago, biosynthetic origins fungal isocyanides remain unknown. Here we report identification family synthases (ICSs) opportunistic human pathogen Aspergillus fumigatus . Comparative metabolomics overexpression or knockout mutants...
Abstract Filamentous fungi differentiate along complex developmental programs directed by abiotic and biotic signals. Currently, intrinsic signals that govern fungal development remain largely unknown. Here we show an endogenously produced secreted oxylipin, 5,8-diHODE, induces cellular differentiation, including lateral branching in pathogenic Aspergillus fumigatus flavus , appressorium formation the rice blast pathogen Magnaporthe grisea . The response is specific to a subset of oxylipins...
The maintenance of sufficient but nontoxic pools metal micronutrients is accomplished through diverse homeostasis mechanisms in fungi. Siderophores play a well established role for iron homeostasis; however, no copper-binding analogs have been found Here we demonstrate that, Aspergillus fumigatus, xanthocillin and other isocyanides derived from the xan biosynthetic gene cluster (BGC) bind copper, impact cellular copper content, significant metal-dependent antimicrobial properties....
Fungal spores contain secondary metabolites that can protect them from a multitude of abiotic and biotic stresses. Conidia (asexual spores) the human pathogen Aspergillus fumigatus synthesize several metabolites, including melanin, which has been reported to be important for virulence in this species protective against UV radiation other fungi. Here, we investigate role melanin diverse isolates A. find variability its ability UV-C or impact zebrafish model invasive aspergillosis two clinical...
Abstract The maintenance of sufficient but non-toxic pools metal micronutrients is accomplished through diverse homeostasis mechanisms in fungi. Siderophores play a well-established role for iron homeostasis; however, no copper-binding analogs have been found Here we demonstrate that Aspergillus fumigatus isocyanides derived from the xan biosynthetic gene cluster (BGC) bind copper, impact cellular copper content, and significant metal-dependent antimicrobial properties. BGC-derived are...