Although autologous nerve grafting is the gold standard treatment of peripheral injuries, several alternative methods have been developed, including conduits that use supportive cells. However, seeding efficacy and viability cells injected in grafts remain unclear. Here, we focused on a novel completely biological, tissue-engineered, scaffold-free conduit.We developed six from human normal dermal fibroblasts using Bio 3D Printer. Twelve adult male rats with immune deficiency underwent...

Autologous nerve grafting is widely accepted as the gold standard treatment for segmental defects. To overcome inevitable disadvantages of original method, alternative methods such tubulization technique have been developed. Several studies investigated characteristics an ideal conduit in terms supportive cells, scaffolds, growth factors, and vascularity. Previously, we confirmed that biological scaffold-free conduits fabricated from human dermal fibroblasts promote regeneration a rat...

The effect of edaravone, a novel free radical scavenger, was assessed functionally and histologically using rat spinal cord contusion model.To investigate the edaravone on neuroprotection after injury in rats.The results immediate physical damage (primary injury), followed by prolonged neural tissue disorder (secondary injury). This secondary process has been suggested to be induced lipid peroxidation. Edaravone reported inhibit peroxidation cerebral ischemia models.Spinal at T10 level with...

Previously, we showed that undifferentiated bone marrow stromal cell (uBMSC) implantation and vessel insertion into a nerve conduit facilitated peripheral regeneration in rodent model. In this study, investigated the efficacy of uBMSC-laden vessel-containing repair segmental defects, using canine Eight beagle dogs were used study. Thirty-millimeter ulnar defects repaired with conduits (right forelimbs, n = 8) or autografts (left 7). group, artery was inserted l-lactide/ε-caprolactone tube,...

Abstract Background The reduction of systemic immunosuppressive agents is essential for the expansion vascularized composite allotransplantation (VCA) in a clinical setting. purpose this study to compare human‐induced pluripotent stem cell‐derived mesenchymal cells (iMSCs) with four other types (human bone marrow‐derived MSCs [BMMSCs], human adipose‐derived [ADMSCs], rat BMMSCs, and ADMSCs) vitro, investigate vivo immunomodulatory effect iMSCs VCA model. Materials methods One Brown Norway...

Abstract Introduction A Bio 3D printed nerve conduit was reported to promote regeneration in a 5 mm gap model. The purpose of this study fabricate conduits suitable for 10 and evaluate their capacity rat sciatic defect Materials Methods Eighteen F344 rats with immune deficiency (9–10 weeks old; weight, 200–250 g) were divided into three groups: group (Bio 3D, n = 6), graft (NG, silicon tube (ST, 6). 12‐mm or transplanted the 10‐mm right nerve. In group, reverse autografting performed an...

Skin is considered to be the most antigenic component of all vascularized composite allotransplantation tissues. However, no studies have used methods other than histological assessment analyze relative antigenicity various components. In this study, we analyzed gene expression investigate each in transplanted limb.Seven Brown Norway rats and 31 Lewis were assigned two groups: an allograft group a syngeneic (control) group. as allogeneic donors, donors recipients. About 13 recipients 12...

Background The development of effective immunosuppressive regimens has resulted in many cases successful hand transplantation. Visual skin inspection and histological evaluation are used to assess the rejection transplants, but these methods largely subjective. In this study, we aimed determine potential microRNAs (miRNAs) as biomarkers for acute vascularized composite allotransplants. Methods allograft group, 7 male Brown-Norway rats (RT1 n ) were donors 13 Lewis l recipients. control 8...

Background Recent studies have indicated that bone marrow‐derived stromal cells (BMSCs) immunomodulatory properties suppress the T cell responses cause graft rejection. The purpose of this study is to evaluate effect recipient BMSCs intravenous infusion for immunomodulation in a rat vascularized composite allotransplantation model. Methods A total nine Wistar (WIS) rats and thirty Lewis (LEW) were used. harvested from three LEW rats. Twenty‐four used as recipients divided randomly into four...

Background: Previous animal studies demonstrated that the sensory and motor functions in ipsilesional upper limbs had been reconstructed by CC7 transfer eventually associated with contralesional brain cortices originally mediated of before brachial plexus injury (BPI). Our hypothesis was same findings would be seen humans. Methods: Four patients total BPI treated were included. Changes locations activated areas primary (M1) somatosensory (S1) corresponding to outputs inputs from investigated...

We investigated the effects of a free radical scavenger, MCI-186 (edaravone), on neuroprotection in rat post-traumatic spinal cord using various doses and routes administration. The injury was produced with weight-drop device. Lipid peroxide formation measured thiobarbituric acid test for malonyldialdehyde (MDA). In first experiment, MDA production untreated reached peak values at 1 h post-trauma, gradually decreased to control levels 7 days. second rats received twice-daily injections (0,...

The nerve root avulsion injury causes decrease of motor neurons in the spinal ventral horn. To investigate motoneuron death after rats, intradural procedure is usually used, although it technically demanding and associated with a risk unexpected cord damage. We have developed new cervical rats investigated validity our procedure.Our using posterior approach pulling C6 outside vertebral foramen without procedures. lateral third mass needed to be resected before root. accomplishment judged by...

Purpose: The rapid death of many spinal motor neurons after nerve root avulsion injury results in limited functional recovery following replantation surgery avulsed nerves into the cord. Therefore, we investigated neuroprotective effect erythropoietin (EPO) on using a rat model. Methods: After C6 injury, EPO (2680 U/kg) was injected subcutaneously once day for 3 consecutive days with various starting time points. At 28 and 56 histological immunohistological investigations were performed....

Abstract Background Limb transplantation is considered to be a treatment option for amputees. Visual skin inspection and histological assessment are used assess rejection, but these techniques largely subjective. Using rat model, we examined the potential of several microRNAs (miRNAs) as objective minimally invasive biomarkers acute rejection transplanted limbs. Methods Three Brown Norway rats 15 Lewis were assigned 2 groups. In allograft group, 3 donors 6 recipients. syngeneic (control) 9...

Background:Replantation of the avulsed nerve root has been proposed for treatment severe brachial plexus injury several decades. However, due to complexity technique and limited functional improvement, practical applications are yet be implemented. Objective:In present study, we investigated effect pretreatment with resveratrol on autografts used replantation surgery in a rat model avulsion. Methods:Resveratrol was performed using an explant culture technique. Two surgical procedures were...

Percutaneous scaphoid screw fixation is a popular treatment for acute fractures with no or minimal displacement. For treating nonunions, however, open reduction and internal bone grafting still the most treatment. through insertion hole has received little attention, although it minimizes damage to surrounding tissues. We report excellent results of six nonunions treated by retrograde percutaneous curettage distal fully threaded headless screw.Six substantial loss were treated, including one...

The avascular necrosis of the metacarpal head is a rare disease. Therefore, although variety surgical treatments were performed in sporadically published case reports, optimal treatment still controversial. Open wedge osteotomy neck was first reported 2002. However, no other reports using same procedure have been published. We presented 13-year-old girl third having treated by open neck. Four years later, she had pain and gained full range motion metacarpophalangeal (MP) joint. plain X-rays...

Dupuytren’s pathologic tissue usually involves the palmar skin and rarely infiltrates into dorsal skin. In this study, we hypothesized that transplantation of a vascularized cutaneous flap harvested from dorsum hand to skin, under which cord existed might be useful for blocking reformation cords decreasing recurrence rate. After removal 2 types islands nourished by digital arterial network including perforating arteries arising arteries. The were transplanted defects in palm fingers had been...

We report the case of a 34-year-old man with total brachial plexus injury that was treated by free functional muscle transplantation to restore simultaneously elbow flexion and finger extension. The had very large belly (12 cm width), which considered anatomically be fusion gracilis adductor longus muscles. Although possessed two major vascular pedicles almost equal diameters, only proximal pedicle anastomosed recipient vessels during surgery, resulting in partial necrosis muscle. Several...

Objective: Percutaneous scaphoid screw fixation is a popular treatment for acute fractures with no or minimal displacement. For treating nonunions, however, open reduction and internal bone grafting still the most treatment. through insertion hole has received little attention, although it minimizes damage to surrounding tissues. We report excellent results of 10 nonunions treated by retrograde percutaneous curettage distal fully threaded headless screw. Materials Methods: Ten substantial...

Summary: Surgical treatment for recurrent, common peroneal neuropathy has not been reported. Herein, we describe a successfully treated case using the proximally based sural fasciocutaneous flap after reneurolysis of adhesive nerve. A 33-year-old man received neurolysis operation entrapment nerve 2 years before first admission in our clinic. Although motor conduction studies showed marked improvement primary operation, patient always required use crutch because severe pain radiating to lower...