A5101581396- Glycosylation and Glycoproteins Research
- Lung Cancer Treatments and Mutations
- Endoplasmic Reticulum Stress and Disease
- Parathyroid Disorders and Treatments
- Galectins and Cancer Biology
- Functional Brain Connectivity Studies
- Metabolism, Diabetes, and Cancer
- Cancer-related Molecular Pathways
- Genetic Syndromes and Imprinting
- Pharmacological Effects of Natural Compounds
- Ubiquitin and proteasome pathways
- Pharmacogenetics and Drug Metabolism
- Inflammasome and immune disorders
- Circular RNAs in diseases
- Cancer Genomics and Diagnostics
- Chronic Kidney Disease and Diabetes
- Glioma Diagnosis and Treatment
- Trauma Management and Diagnosis
- Gastrointestinal motility and disorders
- interferon and immune responses
- Cancer-related molecular mechanisms research
- Pancreatic function and diabetes
- Tracheal and airway disorders
- Immune Response and Inflammation
- Genetic and Kidney Cyst Diseases
Zhujiang Hospital
2025
Southern Medical University
2025
Hainan Medical University
2022-2025
Hainan General Hospital
2019-2025
Zhejiang University Medical College Affiliated Stomatological Hospital
2020-2025
Beijing University of Chinese Medicine
2025
Chinese People's Liberation Army
2022-2024
Chinese PLA General Hospital
2022-2024
First Affiliated Hospital of Guangzhou Medical University
2016-2024
Guangzhou Medical University
2016-2024
Abstract Purpose: To examine the enzyme kinetics of gefitinib and erlotinib metabolism by individual cytochrome P450 (CYP) enzymes, to compare their effects on CYP3A activity, with aim better understand mechanisms underlying pharmacokinetic variability clinical effects. Experimental Design: Enzyme were examined incubating or (1.5-50 μmol/L) recombinant human CYP3A4, CYP3A5, CYP2D6, CYP1A1, CYP1A2, CYP1B1 (10-160 pmol/mL). Their activity comparing midazolam in presence absence liver...
Congenital disorders of glycosylation are genetic syndromes that result in impaired glycoprotein production. We evaluated patients who had a novel recessive disorder glycosylation, with range clinical manifestations included hepatopathy, bifid uvula, malignant hyperthermia, hypogonadotropic hypogonadism, growth retardation, hypoglycemia, myopathy, dilated cardiomyopathy, and cardiac arrest.Homozygosity mapping followed by whole-exome sequencing was used to identify mutation the gene for...
Bupropion is primarily metabolized in human liver by cytochrome P450 (CYP) 2B6, an isoform that shows high interindividual variability expression and catalysis. The aim of this study was to identify mechanisms underlying through comprehensive phenotype-genotype analysis a well-characterized bank (n = 54). There substantial microsomal bupropion hydroxylation activities (over 45-fold) CYP2B6 protein content 288-fold), with excellent correlation between activity values (rs 0.88). mRNA levels...
Mitochondrial dysfunction is implied as a crucial factor in age-related chronic kidney disease. It confirmed that Gli-like transcription 1 (GLIS1) involved renal fibrosis, however, the correlation between mitochondrial disturbances and GLIS1-driven aging are not clearly clarified. Thus, we investigated regulatory mechanism of GLIS1 homeostasis quality control both vivo vitro. The lower expression was identified natural accelerated aged models, accompanied by dysfunctions control, including...
Abstract Human epidermal growth factor receptor 2 (HER2)-positive breast cancer (BC) has been the most challenging subtype of BC, consisting 20% BC with an apparent correlation poor prognosis. Despite that pyrotinib, a new HER2 inhibitor, led to dramatic improvements in prognosis, efficacy pyrotinib monotherapy remains largely restricted due its acquired resistance. Therefore, identifying potential antitumor drug combination amplify therapeutic is pressing necessity. Here, we reported novel...
Graphical Abstract GUP can effectively inhibit the activation of cGAS-STING pathway induced by multiple stimuli and reduce expression type I interferon pro-inflammatory cytokines. Meanwhile also has a good therapeutic effect on sepsis contributed to cecum ligation puncture.
Congenital disorders of glycosylation (CDG) are rare genetic due to impaired glycosylation. The patients with subtypes CDG-Ia and CDG-Ib have mutations in the genes encoding phosphomannomutase 2 (PMM2) phosphomannose isomerase (MPI or PMI), respectively. PMM2 (mannose 6-phosphate → mannose 1-phosphate) MPI ⇔ fructose 6-phosphate) deficiencies reduce metabolic flux (Man-6-P) into glycosylation, resulting unoccupied N-glycosylation sites. Both compete for same substrate, Man-6-P. Daily doses...
N-Glycanase 1, encoded by NGLY1, catalyzes the deglycosylation of misfolded N-linked glycoproteins retrotranslocated into cytosol.We identified nine cases with mutations in NGLY1.The patients show developmental delay, seizures, peripheral neuropathy, abnormal liver function and alacrima (absence tears).The NGLY1 resulted absence N-glycanase 1 protein patient-derived fibroblasts.Applying a recently established cellular deglycosylationdependent Venus fluorescence assay, we found that patient...
Inorganic phosphate (Pi) is an essential nutrient for human health. Due to the changes in our dietary pattern, Pi overload engenders systemic phosphotoxicity, including excessive Pi-related vascular calcification and chronic tissue injury. The molecular mechanisms of seemingly distinct phenotypes remain elusive. In this study, we investigated Pi-mediated cellular response HEK293 HeLa cells. We found that abnormally high directly mediates diverse toxicity a dose-dependent manner. Up 10 mM...
Abstract Background Patients with sepsis resulting in acute lung injury (ALI) usually have increased mortality. Ferroptosis is a vital regulator sepsis-induced ALI. Exploring the association of ferroptosis and ALI crucial for management Methods Whole blood was collected from patients. Mice were treated cecal ligation puncture (CLP) to model sepsis. Primary murine pulmonary microvascular endothelial cells lipopolysaccharide as cell model. evaluated by analyzing levels iron, malonaldehyde,...
Patients with congenital disorder of glycosylation (CDG), type Ib (MPI-CDG or CDG-Ib) have mutations in phosphomannose isomerase (MPI) that impair and lead to stunted growth, liver dysfunction, coagulopathy, hypoglycemia, intestinal abnormalities. Mannose supplements correct hypo-glycosylation most symptoms by providing man-nose-6-P (Man-6-P) via hexokinase. We generated viable Mpi hypomorphic mice residual enzymatic activity comparable patients, but surprisingly, these appeared completely...
Congenital disorders of glycosylation (CDGs) result from mutations in various N-glycosylation genes. The most common type, phosphomannomutase-2 (PMM2)-CDG (CDG-Ia), is due to deficient PMM2 (Man-6-P → Man-1-P). Many patients die recurrent infections, but the mechanism unknown. We found that glycosylation-deficient patient fibroblasts have less intercellular adhesion molecule-1 (ICAM-1), and because its role innate immune response, we hypothesized reduction might help explain infections CDG...
Glycyrrhiza uralensis polysaccharides (GUP) has biological activities such as immune regulation, anti-tumor, and liver protection, good development prospects in the fields of medicines health products, but its specific mechanism action is not yet clear. In our study, we confirmed that GUP can effectively inhibit activation NLRP3 inflammasome. Mechanistically, blocked inflammasome by significantly inhibiting oligomerization speckle aggregation apoptosis-related protein ASC. addition,...
Abstract Background The cGAS-STING signaling pathway is an essential section of the natural immune system. In recent years, increasing number studies have shown a strong link between abnormal activation pathway, mediated by nucleic acid receptor cGAS, and development progression autoimmune diseases. Therefore, it important to identify effective compound specifically downregulate this for disease. Methods effect Glabridin (Glab) was investigated in BMDMs Peripheral blood mononuclear cell...