A5040190247- Leprosy Research and Treatment
- Mycobacterium research and diagnosis
- Infectious Diseases and Tuberculosis
- Tuberculosis Research and Epidemiology
- Peripheral Neuropathies and Disorders
- Hematological disorders and diagnostics
- Psoriasis: Treatment and Pathogenesis
- Ocular Diseases and Behçet’s Syndrome
- Pharmacological Effects of Natural Compounds
- Erythrocyte Function and Pathophysiology
- Multiple Myeloma Research and Treatments
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Sphingolipid Metabolism and Signaling
- T-cell and B-cell Immunology
- Botulinum Toxin and Related Neurological Disorders
- Chronic Lymphocytic Leukemia Research
- Herpesvirus Infections and Treatments
- Toxin Mechanisms and Immunotoxins
- Ophthalmology and Eye Disorders
- Yersinia bacterium, plague, ectoparasites research
- Acne and Rosacea Treatments and Effects
- Health, Nursing, Elderly Care
- Immunotherapy and Immune Responses
- Oral Health Pathology and Treatment
Fundação Oswaldo Cruz
2015-2024
Faculdades Oswaldo Cruz
1988-2023
Universidade Vale do Rio Doce
2020
Universidade Federal de Juiz de Fora
2020
Weatherford College
2020
Central Leprosy Teaching & Research Institute
2000-2017
Hospital Universitário Pedro Ernesto
2016
Universidade Federal do Rio de Janeiro
1980-2012
National Hansen's Disease Program
2010
Louisiana State University
2010
Thalidomide selectively inhibits the production of human monocyte tumor necrosis factor alpha (TNF-alpha) when these cells are triggered with lipopolysaccharide and other agonists in culture. 40% inhibition occurs at clinically achievable dose drug 1 micrograms/ml. In contrast, amount total protein individual proteins labeled [35S]methionine expressed on SDS-PAGE not influenced. The amounts interleukin beta (IL-1 beta), IL-6, granulocyte/macrophage colony-stimulating produced by monocytes...
Immunologic and clinical manifestations of erythema nodosum leprosum (ENL) their response to thalidomide therapy were evaluated. Circulating tumor necrosis factor-α (TNFα) levels assayed in serum obtained from lepromatous leprosy patients at diagnosis, during multidrug therapy, the onset ENL episodes, treatment with thalidomide. Patients systemic demonstrated highest TNFα levels, which decreased significantly treatment. Serum nonreactional was associated mild flu-like symptoms local...
Interfering with Interferons Infections Mycobacteria, including Mycobacterium leprae or M. tuberculosis , vary substantially in their clinical presentation. For instance, some cases of the infection is self-healing very few lesions. In contrast, people experience disseminated form, where skin lesions abound and bacteria are abundant. patients infected Teles et al. (p. 1448 published online 28 February) found that disease associates a type I interferon gene signature, whereas form II...
We report on the characteristics of cells in cutaneous lesions and blood 21 patients with lepromatous, tuberculoid, intermediate forms leprosy. A large proportion infiltrates lepromatous consist macrophages heavily parasitized Mycobacterium leprae. The T are devoid OKT4/Leu 3a-positive ("helper") almost exclusively OKT8/Leu 2a-positive ("suppressor") populations. In contrast, tuberculoid contain well-organized epithelioid giant-cell granulomas only remnants bacilli, predominant cell is from...
The predilection of Mycobacterium leprae (ML) for Schwann cells (SCs) leads to peripheral neuropathy, a major concern in leprosy. Highly infected SCs lepromatous leprosy nerves show foamy, lipid-laden appearance; but the origin and nature these lipids, as well their role leprosy, have remained unclear. data presented that ML has pronounced effect on host-cell lipid homeostasis through regulation droplet (lipid bodies, LD) biogenesis intracellular distribution. Electron microscopy...
Background Leprosy remains an important public health problem in Brazil where 28,761 new cases were diagnosed 2015, the second highest number of detected globally. The disease is caused by Mycobacterium leprae, a pathogen spread patients with multibacillary (MB) leprosy. This study was designed to identify population groups most at risk for MB Brazil, contributing ideas early diagnosis and leprosy control. Methods A national databank reported (2001–2013) used evaluate epidemiological...
Background This study aimed to evaluate the risk factors associated with developing leprosy among contacts of newly-diagnosed patients. Methodology/Principal Findings A total 6,158 and 1,201 patients cohort who were diagnosed treated at Leprosy Laboratory Fiocruz from 1987 2007 included. The contact variables analyzed sex; age; educational income levels; blood relationship, if any, index case; household or non-household relationship; length time close association receipt bacillus...
Leprosy is a chronic human disease caused by the yet-uncultured pathogen Mycobacterium leprae. Although readily curable with multidrug therapy (MDT), over 200,000 new cases are still reported annually. Here, we obtain M. leprae genome sequences from DNA extracted directly patients' skin biopsies using customized protocol. Comparative and phylogenetic analysis of 154 genomes 25 countries provides insight into evolution antimicrobial resistance, uncovering lineages phylogeographic trends, most...
Erythema nodosum leprosum (ENL) is a severe multisystem immune mediated complication of borderline lepromatous leprosy and leprosy. ENL associated with skin lesions, neuritis, arthritis, dactylitis, eye inflammation, osteitis, orchitis, lymphadenitis nephritis. The treatment requires immunosuppression, which often required for prolonged periods time may lead to serious adverse effects. its increased mortality economic hardship. Improved, evidence-based treatments are needed; however,...
Leprosy presents as a clinical and immunological spectrum of disease. With the use gene expression profiling, we observed that distinction in correlates with accurately classifies form Genes belonging to leukocyte immunoglobulin-like receptor (LIR) family were significantly up-regulated lesions lepromatous patients suffering from disseminated infection. In functional studies, LIR-7 suppressed innate host defense mechanisms by shifting monocyte production interleukin-12 toward interleukin-10...
Nerve damage is a clinical hallmark of leprosy and major source patient morbidity. We investigated the possibility that human Schwann cells are susceptible to cell death through activation Toll-like receptor 2 (TLR2), pattern recognition innate immune system. TLR2 was detected on surface line ST88-14 cultured primary cells. Activation cultures with agonist, synthetic lipopeptide comprising N-terminal portion putative Mycobacterium leprae 19-kDa lipoprotein, triggered an increase in number...
10 patients with borderline and lepromatous leprosy were selected for a prolonged trial recombinant interferon gamma (rIFN-gamma). Patients received 30 micrograms intradermally six injections over 9-d period, then either 100 every 1 mo or 2 wk 5 (total, 1.2 mg). Erythema nodosum leprosum (ENL) was induced in 60% of the within 6-7 mo, as compared an incidence 15% per year multiple drug therapy alone. The mean whole-body reduction bacterial index first 6 0.9 log units. Cutaneous induration at...
Single-nucleotide polymorphisms within the genes coding for tumor necrosis factor (TNF)-alpha and interleukin (IL)-10 have been associated with several infectious diseases. To determine whether such are leprosy, genotyping was performed at -308 -238 positions of promoter TNF-alpha gene in 210 191 patients multibacillary (MB) respectively; 90 79 paucibacillary (PB) leprosy; 92 control subjects. For -592 -819 IL-10 gene, 143 MB PB 62 subjects were included analysis. TNF2 allele frequency...
Antigen and mitogen-induced gamma interferon (gamma-IFN) production was studied in peripheral blood mononuclear cells from 34 leprosy patients. 17 of 18 lepromatous borderline patients (LL BL) failed to release gamma-IFN response specific antigen (Mycobacterium leprae) displayed reduced responses mitogen (concanavalin A) stimulation. In contrast, six tuberculoid (TT BT) produced considerable levels under the same experimental conditions. Normal controls respond M. leprae most good...