A5018687587- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Infectious Diseases and Tuberculosis
- Leprosy Research and Treatment
- Parasites and Host Interactions
- Travel-related health issues
- Malaria Research and Control
- Research on Leishmaniasis Studies
- Fungal Infections and Studies
- Trypanosoma species research and implications
- Diagnosis and treatment of tuberculosis
- Parasite Biology and Host Interactions
- Mosquito-borne diseases and control
- Parasitic Infections and Diagnostics
- Salivary Gland Disorders and Functions
- Viral Infections and Vectors
- Parasitic Diseases Research and Treatment
- Parasitic infections in humans and animals
- Vector-borne infectious diseases
- Hepatitis Viruses Studies and Epidemiology
- Veterinary medicine and infectious diseases
- Insects and Parasite Interactions
- Global Maternal and Child Health
- Humic Substances and Bio-Organic Studies
Ludwig-Maximilians-Universität München
2014-2023
LMU Klinikum
2007-2020
Kwame Nkrumah University of Science and Technology
2009-2014
St George's, University of London
2014
Universitätsklinikum Gießen und Marburg
2007-2010
Bernhard Nocht Institute for Tropical Medicine
2002-2009
Kumasi Centre for Collaborative Research in Tropical Medicine
2009
University of Würzburg
1994-2006
Deutsche Lepra- und Tuberkulosehilfe
1994-1998
Heidelberg University
1997
Although the virulences and host ranges differ among members of Mycobacterium tuberculosis complex (TBC; M. tuberculosis, africanum, canettii, microti, bovis, bovis BCG), commercially available molecular assays cannot differentiate these organisms because genetic identities their 16S rRNA gene sequences. Comparative genomic analyses with complete DNA sequence H37Rv has provided information on regions difference (RD 1 to RD 16) deleted in TBC other than tuberculosis. To determine whether...
Leprosy is a chronic human disease caused by the yet-uncultured pathogen Mycobacterium leprae. Although readily curable with multidrug therapy (MDT), over 200,000 new cases are still reported annually. Here, we obtain M. leprae genome sequences from DNA extracted directly patients' skin biopsies using customized protocol. Comparative and phylogenetic analysis of 154 genomes 25 countries provides insight into evolution antimicrobial resistance, uncovering lineages phylogeographic trends, most...
Background. Antimicrobial killing in mycobacterial infections may be accompanied by (transient) clinical deterioration, known as paradoxical reaction. To search for patterns reflecting such reactions the treatment of Buruli ulcer (Mycobacterium ulcerans infection), evolution lesions patients treated with antimicrobials was prospectively assessed. Methods. The lesion size participants BURULICO antimicrobial trial (with ≤10 cm cross-sectional diameter) assessed careful palpation and recorded...
The only available vaccine that could be potentially beneficial against mycobacterial diseases contains live attenuated bovine tuberculosis bacillus (Mycobacterium bovis) also called Bacillus Calmette-Guérin (BCG). Even though the BCG is still widely used, results on its effectiveness in preventing are partially contradictory, especially regarding Buruli Ulcer Disease (BUD). aim of this case-control study to evaluate possible protective effect vaccination BUD.The present was performed three...
The magnitude of the current Zika virus (ZIKV) epidemic has led to a declaration Public Health Emergency International Concern by WHO. Findings viable viral particles in semen for several weeks are corroborating reports sexual transmission ZIKV. Serious consequences positive diagnostic result particularly pregnant patient calling precise tools also at later time points after infection. Currently, recommendations suggest period direct detection 5 7 days onset symptoms serum or plasma, and up...
Abstract Urogenital schistosomiasis was diagnosed in a man from Germany who had never traveled outside Europe. He likely acquired the infection Corsica, France, but did not swim Cavu River, which linked to previous outbreak. This case highlights that transmission of Corsica is ongoing.
A sample of 124 isoniazid (INH)-resistant and 88 susceptible strains Mycobacterium tuberculosis complex from south, central, west Africa was analyzed by direct sequence analysis PCR-restriction fragment length polymorphism their catalase-peroxidase (katG) genes. Point mutations at codon 315 were found in the genomes 64% INH-resistant strains, but no complete deletions identified. Mutations 463 independent INH resistance linked to geographic origins strains.
Several diagnostic laboratory methods are available for case confirmation of Buruli ulcer disease. This study assessed the sensitivity various tests in relation to clinical presentation disease, type specimen, and treatment history.Swab samples, 3-mm punch biopsy tissue specimens, surgically excised specimens from 384 individuals with suspected disease were obtained at 9 different sites Ghana evaluated dry reagent-based polymerase chain reaction (PCR), microscopic examination, culture,...
SUMMARY Introduction. Several reports indicate high prevalences of both onchocerciasis and epilepsy in some regions Africa. This raises the question whether these diseases are associated. We therefore investigated people with and/or living an area Tanzania endemic for Onchocerca volvulus ( O. ). Methods. collected clinical information, skin snips, blood from 300 individuals, cerebrospinal fluid (CSF) 197. Participants were allocated to 4 groups consisting n =135), those either =61) or only...
Malaria has been shown to change blood counts. Recently, a few studies have investigated the alteration of peripheral monocyte-to-lymphocyte count ratio (MLCR) and neutrophil-to-lymphocyte (NLCR) during infection with Plasmodium falciparum. Based on these findings this study investigates predictive values alterations malaria across different sub-populations.Cases controls admitted Department Infectious Diseases Tropical Medicine from January 2000 through December 2010 were included in...
The aim of this study was to assess the spectrum imported infectious diseases (IDs) among patients consulting University Munich, Germany, between 1999 and 2014 after being in sub-/tropics. analysis investigated complete data sets 16,817 diseased German travelers (2,318 business travelers, 4,029 all-inclusive 10,470 backpackers) returning from Latin America (3,225), Africa (4,865), or Asia (8,727), 977 immigrants, originating same regions (112, 654 211 respectively). most frequent symptoms...
PLOS Neglected Tropical Diseases 1 August 2012 | Volume 6 Issue 8 e1756.Also available at www.plosntds.org
Background As the major burden of Buruli ulcer disease (BUD) occurs in remote rural areas, development point-of-care (POC) tests is considered a research priority to bring diagnostic services closer patients. Loop-mediated isothermal amplification (LAMP), simple, robust and cost-effective technology, has been selected as promising POC test candidate. Three BUD-specific LAMP assays are available date, but various technical challenges still hamper decentralized application. To overcome...
Purpose Chagas disease (CD) has become a global health issue mainly due to migration. Germany lacks surveillance data and is home large Latin American immigrant population. Recognising that Bolivia the country with highest CD prevalence in America, this cross-sectional, descriptive pilot study investigated associated factors among citizens of Bolivian origin living Munich, Germany. Methods Participants completed questionnaire order collect socioeconomic health-related data. In addition,...
Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention non-vectorial transmission as well treatment. This study compared four distinct PCR methods detection Trypanosoma cruzi DNA the use in well-equipped routine diagnostic laboratories. was extracted T. cruzi-positive and negative patients' blood samples cultured cruzi, rangeli Leishmania spp. One conventional two real-time targeting a...
ABSTRACT The Mycobacterium tuberculosis complex includes M. , bovis africanum and microti . Most clinical isolates are or These species can be distinguished by phenotypes genotypes. However, there is no simple definition of some authors question the validity this species. We analyzed 17 human from Sierra Leone, identified as biochemical growth characteristics. sequenced polymorphic genes intergenic regions. amplified DNA six loci with variable numbers tandem repeats (VNTRs) determined exact...
ABSTRACT After tuberculosis and leprosy, Buruli ulcer (BU), caused by Mycobacterium ulcerans , is the third most common mycobacterial disease in immunocompetent humans. The occurs tropical countries, with foci West Africa, Central western Pacific. BU defined as an infectious involving skin subcutaneous adipose tissue characterized a painless nodule, papule, plaque, or edema, evolving into undermined edges often leading to invalidating sequelae. Due fundamental lack of understanding modes...
Infections with larvae of Taenia crassiceps are rare in humans and have mostly affected patients acquired immunodeficiency syndrome. We report the first case a patient malignancy (non-Hodgkin's lymphoma) infection subcutis muscles hand forearm. Surgery antiparasitic chemotherapy led to complete cure.
Background. Because of the multifaceted clinical presentation Buruli ulcer disease, misclassification clinically diagnosed cases may occur frequently. Laboratory tests for confirmation suspected include microscopic examination, culture, polymerase chain reaction (PCR), and histopathologic examination. However, only test usually available in areas endemicity, has a low sensitivity. Methods. To make highly sensitive diagnostic method locally available, dry reagent–based PCR (DRB-PCR), which is...