A5005385490- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Polysaccharides Composition and Applications
- Osteoarthritis Treatment and Mechanisms
- Influenza Virus Research Studies
- Respiratory viral infections research
- Bone and Dental Protein Studies
- Collagen: Extraction and Characterization
- Neuroscience of respiration and sleep
- Carbohydrate Chemistry and Synthesis
- Protease and Inhibitor Mechanisms
- Infant Health and Development
- Animal Disease Management and Epidemiology
- Gut microbiota and health
- Plant Disease Resistance and Genetics
- Diabetes Management and Research
- Medical and Biological Sciences
- Rheumatoid Arthritis Research and Therapies
- Dendrimers and Hyperbranched Polymers
- Oral microbiology and periodontitis research
- Cervical Cancer and HPV Research
- Fibroblast Growth Factor Research
- Salivary Gland Disorders and Functions
- Veterinary Equine Medical Research
- Antimicrobial Resistance in Staphylococcus
Lancaster University
2009-2024
Newcastle Dental Hospital
1989-1993
Illinois College
1991
University of Lübeck
1987
Magnetic resonance imaging was used to estimate the volume of tongue, oropharynx, and oral cavity in 19 adults. Each subject imaged had estimations made from coronal sagittal orientations. Volume found by measuring area a series images then multiplying thickness each slice gap between series. Mean tongue volumes 71.2cc (coronal) 79.3cc (sagittal) were found. The estimated be reproducible orientation equally good for defining anatomy oropharynx. Tongue correlate well with body weight, r =...
Principal component analysis (PCA) is a method of simplifying complex datasets to generate lower number parameters, while retaining the essential differences and allowing objective comparison large numbers datasets. Glycosaminoglycans (GAGs) are class linear sulfated carbohydrates with diverse sequences consequent conformation structure. Here, PCA applied three problems in GAG research: (i) distinguishing origins heparin preparations, (ii) structural derivatives, (iii) classification...
A previously published method for the analysis of glycosaminoglycan disaccharides by high pH anion exchange chromatography (Midura,R.J., Salustri,A., Calabro,A., Yanagishita,M. and Hascall,V.C. (1994), Glycobiology,4, 333-342) has been modified calibrated chondroitin dermatan sulfate oligosaccharides up to hexasaccharide in size hyaluronan hexadecasaccharide. For chain length controls elution position; however, times primarily depend upon level sulfation, although hence charge density plays...
The chondroitin sulphate (CS) linkage regions have been isolated from human articular cartilage aggrecan (from 10- to 72-year-olds) by ABC endolyase digestion and size-exclusion chromatography. Linkage region hexasaccharides characterized their abundance estimated high-pH anion-exchange basic structure for the CS oligosaccharides identified is as follows: ΔUA(β1–3)GalNAc[0S/4S/6S](β1–4)GlcA(β1–3)Gal[0S/6S](β1–3)Gal(β1–4)Xyl, where ΔUA represents 4,5-unsaturated hexuronic acid, 4S 6S...
The chondroitin sulphate (CS) linkage regions have been isolated from human articular cartilage aggrecan (from 10- to 72-year-olds) by ABC endolyase digestion and size-exclusion chromatography. Linkage region hexasaccharides characterized their abundance estimated high-pH anion-exchange basic structure for the CS oligosaccharides identified is as follows: ΔUA(β1–3)GalNAc[0S/4S/6S](β1–4)GlcA(β1–3)Gal[0S/6S](β1–3)Gal(β1–4)Xyl, where ΔUA represents 4,5-unsaturated hexuronic acid, 4S 6S...
Summary The ultraviolet circular dichroism (CD) spectra of commercial low-molecular-weight heparins, heparinoids and other anticoagulant preparations have been recorded between 180 260 nm. Principal component analysis the allowed their differentiation into a number groups related to means production reflecting structural changes introduced by each process. findings suggest that CD provides complementary technique for rapid heparin preparations.
Chondroitin sulfates were fragmented using the enzymes chondroitin sulfate ABC endolyase and ACII lyase; both disaccharide tetrasaccharide fragments isolated after reduction to corresponding 2-deoxy-2-N-acetylamino-D-galactitol (GalNAc-ol) form. These have structures: Delta UA(beta 1--3)GalNAc4S-ol, 1--3)GalNAc6S-ol, UA2S(beta 1--3)GalNAc4S(beta 1--4)L-IdoA(alpha 1--4)GlcA(beta 1--3)GalNAc6S(beta 1--3)GalNAc4S-ol where UA represents a 4,5-unsaturated hexuronic acid...
Abstract Influenza C is not included in the annual seasonal influenza vaccine, and has historically been regarded as a minor respiratory pathogen. However, recent work highlighted its potential role cause of pneumonia infants. We performed nasopharyngeal or nasal swabbing and/or serum sampling ( n = 148) Lancaster, UK, over winter 2014–2015. Using enzyme-linked immunosorbent assay (ELISA), we obtain seropositivity 77%. By contrast, only 2 individuals, both asymptomatic adults, were...
We report the isolation, characterization and quantification of five octasaccharides, four hexasaccharides two tetrasaccharides, derived from chondroitin sulphate (CS) linkage region 6-8-year-old bovine articular cartilage aggrecan, following digestion with ABC endolyase. Using a novel high-pH anion-exchange chromatography (HPAEC) method, in conjunction one- two-dimensional 1H-NMR spectroscopy, we have identified basic structure for CS aggrecan:...
The common bacterial toxins hypothesis of sudden infant death syndrome (SIDS) is that nasopharyngeal can trigger events leading to in infants with absent/low levels antibody neutralise the toxins. aim this study was investigate carriage Staphylococcus aureus and determine immunity first year life toxic shock toxin (TSST-1) staphylococcal enterotoxin C (SEC). Both have been implicated SIDS cases. Seventy-three mothers their (39 males 34 females) were enrolled onto study. had birth dates...
Conference Article| October 01 1990 Effects of catabolic and anabolic cytokines on proteoglycan biosynthesis in young, old osteoarthritic canine cartilage GILLIAN VENN; VENN 1Department Biochemistry, Kennedy Institute Rheumatology, 6 Bute Gardens, London W6 7DW, U.K. Search for other works by this author on: This Site PubMed Google Scholar ROBERT M. LAUDER; LAUDER TIMOTHY E. HARDINGHAM; HARDINGHAM HELEN MUIR Biochem Soc Trans (1990) 18 (5): 973–974. https://doi.org/10.1042/bst0180973 Article...
Chondroitin and dermatan sulfate (CS DS) chains were isolated from bovine tracheal cartilage pig intestinal mucosal preparations fragmented by enzymatic methods. The oligosaccharides studied include a disaccharide hexasaccharides chondroitin ABC lyase digestion as well trisaccharides already present in some commercial preparations. In addition, other generated tetrasaccharides chemical removal of nonreducing terminal residues. Their structures examined high‐field 1 H 13 C NMR spectroscopy,...
We investigated the effect of carriage Staphylococcus aureus in later stages pregnancy on levels antibody specific to S. toxins, staphylococcal enterotoxin B (SEB), C (SEC) and toxic shock syndrome toxin-1 (TSST-1), cord blood breast milk also explored relationship between antenatal serum blood. Nasopharyngeal swabs stool samples were collected two occasions, from 96 women, during last 6 weeks pregnancy. Samples cultured isolates identified. Antenatal same women their infants analysed for...
Aim: To screen infant urine for staphylococcal pyrogenic toxins as a possible marker toxigenic, transient bacteraemia. Methods: Nasopharyngeal swabs, skin stool and samples were collected from 30 infants at 2 weeks, 10 weeks 7 months of age when the healthy, they had cold. Samples cultured Staphylococcus aureus isolates identified. Isolates tested production enterotoxin B (SEB), C (SEC) toxic shock syndrome toxin (TSST-1). Urine analysed presence these by ELISA. Results: carriage S decreased...
Five octasaccharides derived from the protein carbohydrate linkage region of chondroitin sulphate (CS) have been isolated large aggregating proteoglycan (aggrecan) extracted bovine articular cartilage 6‐year‐old to 8‐year‐old animals. Following purification aggrecan attached CS chains were digested with ABC endolyase and subsequently released core by β‐elimination. The individual oligosaccharides purified strong anion‐exchange chromatography their structures determined very high‐field...
We report the isolation, characterization and quantification of five octasaccharides, four hexasaccharides two tetrasaccharides, derived from chondroitin sulphate (CS) linkage region 6-8-year-old bovine articular cartilage aggrecan, following digestion with ABC endolyase. Using a novel high-pH anion-exchange chromatography (HPAEC) method, in conjunction one- two-dimensional 1H-NMR spectroscopy, we have identified basic structure for CS aggrecan:...