A5066666004- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Lipid metabolism and disorders
- Cytokine Signaling Pathways and Interactions
- Radiopharmaceutical Chemistry and Applications
- Platelet Disorders and Treatments
- Kruppel-like factors research
- Acute Myeloid Leukemia Research
- Renal Diseases and Glomerulopathies
- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- CAR-T cell therapy research
- Respiratory viral infections research
- interferon and immune responses
- Chronic Lymphocytic Leukemia Research
- IL-33, ST2, and ILC Pathways
- Blood properties and coagulation
- Hemoglobinopathies and Related Disorders
- RNA Interference and Gene Delivery
- Protein Tyrosine Phosphatases
- Advanced biosensing and bioanalysis techniques
- Endoplasmic Reticulum Stress and Disease
- Nanowire Synthesis and Applications
- Amino Acid Enzymes and Metabolism
- Chronic Myeloid Leukemia Treatments
Boston Children's Hospital
2023-2025
Harvard University
2023-2025
Boston Children's Museum
2024
de Duve Institute
2016-2023
Ludwig Cancer Research
2016-2022
UCLouvain
2012-2022
Walloon Excellence in Lifesciences and Biotechnology
2019-2020
The immune system eliminates pathogen intruders such as viruses and bacteria. To recruit effectors to virus-infected cells, we conjugated a small molecule, the influenza neuraminidase inhibitor zanamivir, nanobody that recognizes kappa light chains of mouse immunoglobulins. This adduct was designed achieve half-life extension zanamivir through complex formation with much-larger immunoglobulins in circulation. moiety targets whereas anti-kappa component simultaneously delivers polyclonal...
The purpose of the present work was to identify catalytic activity AGXT2L1 and AGXT2L2, two closely related, putative pyridoxal-phosphate-dependent enzymes encoded by vertebrate genomes. existence bacterial homologues (40-50% identity with AGXT2L2) forming bi- or tri-functional proteins a kinase belonging family aminoglycoside phosphotransferases suggested that AGXT2L2 acted on phosphorylated aminated compounds. Vertebrate genomes were found encode homologue (AGPHD1) these kinases, which...
Mutant calreticulin (CALR) proteins resulting from a -1/+2 frameshifting mutation of the CALR exon 9 carry novel C-terminal amino acid sequence and drive development myeloproliferative neoplasms (MPNs). CALRs were shown to interact with activate thrombopoietin receptor (TpoR/MPL) in same cell. We report that mutant are secreted can be found patient plasma at levels up 160 ng/mL, mean 25.64 ng/mL. Plasma is complex soluble transferrin 1 (sTFR1) acts as carrier protein increases half-life....
Abstract The glycoproteins MICA and MICB are upregulated on the surface of cells undergoing stress, for instance due to (viral) infection or malignant transformation. MICA/B ligands activating receptor NKG2D, found cytotoxic immune like NK cells, CD8+ T γδ cells. Upon engagement these activated eradicate MICA/B-positive targets, assisted by secretion cytokines. Nanobodies, VHHs, derived from variable regions camelid heavy-chain only immunoglobulins. Nanobodies characterized their small size,...
Two anti-transferrin receptor (TfR) nanobodies, V H H123 specific for mouse TfR and H188 human (huTfR) were used to track transplants non-invasively by PET/CT in models, without the need genetic modification of transferred cells. We provide a comparison specificity kinetics PET signals acquired when using nanobodies radiolabeled with 89 Zr, 64 Cu 18 F. knock-in that expresses ectodomain (huTfR +/+ ) as source into C57BL/6 recipients show detects such PET/CT. Conversely, huTfR can be imaged...
Two anti-transferrin receptor (TfR) nanobodies, V H H123 specific for mouse TfR and H188 human (huTfR) were used to track transplants non-invasively by PET/CT in models, without the need genetic modification of transferred cells. We provide a comparison specificity kinetics PET signals acquired when using nanobodies radiolabeled with 89 Zr, 64 Cu 18 F. knock-in that expresses ectodomain (huTfR +/+ ) as source into C57BL/6 recipients show detects such PET/CT. Conversely, huTfR can be imaged...
Influenza remains a significant public health threat. Both monoclonal antibodies and small-molecule inhibitors can target the influenza surface glycoproteins hemagglutinin (HA) or neuraminidase (NA) for prevention treatment of influenza. Here, we combine strengths anti-influenza small molecules by site-specific conjugation NA inhibitor zanamivir to MEDI8852, an HA-specific fully human antibody. MEDI8852 targets conserved stem region HA inhibits HA-mediated fusion viral host cell membranes....
Primary familial and congenital polycythemia is characterized by erythropoietin hypersensitivity of erythroid progenitors due to germline nonsense or frameshift mutations in the receptor gene. All so far described lead truncation C-terminal sequence that contains negative regulatory domains. Their removal presented as sufficient cause phenotype. Here we provide evidence for a new mechanism whereby presence novel sequences generated required phenotype rather than just extensive resulting from...
Dimerization of the thrombopoietin receptor (TpoR) is necessary for activation and downstream signaling through activated Janus kinase 2. We have shown previously that different orientations transmembrane (TM) helices within a dimer can lead to outputs. Here we addressed structural basis mutations S505N W515K induce myeloproliferative neoplasms. show using in vivo bone marrow reconstitution experiments ligand-independent TpoR by TM asparagine (Asn) substitutions proportional proximity Asn...
Bispecific antibody engagers are fusion proteins composed of a nanobody that recognizes immunoglobulin kappa light chains (
Two anti-transferrin receptor (TfR) nanobodies, V H H123 specific for mouse TfR and H188 human (huTfR) were used to track transplants non-invasively by PET/CT in models, without the need genetic modification of transferred cells. We provide a comparison specificity kinetics PET signals acquired when using nanobodies radiolabeled with 89 Zr, 64 Cu 18 F. knock-in that expresses ectodomain (huTfR +/+ ) as source into C57BL/6 recipients show detects such PET/CT. Conversely, huTfR can be imaged...