Trueperella pyogenes is a commensal and opportunistic pathogen that normally causes mastitis, liver abscesses pneumonia of economically important livestock. To develop efficacious potent vaccine against T. pyogenes, chimeric gene DNA vaccines were constructed encapsulated in chitosan nanoparticles (pPCFN-CpG-CS-NPs).The pPCFN-CpG-CS-NPs consists the plo, cbpA, fimA, nanH CpG ODN1826. It was produced with good morphology, high stability, mean diameter 93.58 nm, zeta potential + 5.27 mV....

Predatory natural enemies play key functional roles in biological control. Abundant predatory arthropod species have been recorded tea plantation ecosystems. However, few studies comprehensively evaluated the control effect of arthropods on pests field. We performed a 1-year field investigation and collected canopy. A total 7931 individuals were collected,

Spiders are among the predominant predatory arthropods in rice field ecosystems. Although potential of spiders for controlling pests during growth stages is well known, few studies have focused on overwintering habits after harvesting. In present study, we aimed to evaluate stubble as an microhabitat winter-fallowed fields. To this end, investigated arthropod community composition and analyzed prey spectra common predators fields Nanchong City, Sichuan Province, China. The results showed...

Microsatellite or simple sequence repeat (SSR) instability within genes can induce genetic variation. The SSR signatures remain largely unknown in different clades Euarchontoglires, one of the most successful mammalian radiations. Here, we conducted a genome-wide characterization microsatellite distribution patterns at taxonomic levels 153 Euarchontoglires genomes. Our results showed that abundance and density SSRs were significantly positively correlated with primate genome size, but no...

Trueperella pyogenes (T. pyogenes) is a crucial opportunistic pathogen normally causing mastitis, abscesses and pneumonia in economically important ruminants. Although only one commercial vaccine of T. currently obtainable, its immunoprotective effect limited. Pyolysin (PLO) the most predominant virulence factor highly expressed an excellent target for development novel vaccines against pyogenes. In this study, we designed heterologous prime-boost vaccination scheme combining DNA pVAX1-PLO...

Recently, an increasing number of microsatellites or simple sequence repeats (SSRs) have been found and characterized from transcriptomes. Such SSRs can be employed as putative functional markers to easily tag corresponding genes, which play important role in biomedical studies genetic analysis. However, the transcriptome-derived for giant panda (Ailuropoda melanoleuca) are not yet available. In this work, we identified 20 tetranucleotide microsatellite loci a transcript database generated...

Abstract Insect gut microbiota and their metabolites play a significant role in the shaping of hosts' diets feeding habits. We conducted 16S rDNA amplicon sequencing on specialist blister beetle larvae that feed locust eggs artificial food at different instars, to explore relationship between specialized habit larvae. There is no difference microbial structure among second fourth instar under same rearing conditions, but first was significantly from fed by diets. Bacteria associated with...

The complete mitochondrial genome of L. edwardsi was first sequenced and characterized. 16,284 bases in length the composition arrangement its genes are analogous to most other rodents. nucleotide sequence date 12 heavy-strand protein-coding 26 Muridae species were used for phylogenetic analyses. Trees constructed using Maximum Likelihood, Neighbor Joining Minimum Evolution demonstrated that closer genus Niviventer than Rattus. Combing previous research, it suggests Edward's long-tailed rat...

Spiders are the most dominant predatory natural enemies of insect pests in tea plantation ecosystem. There has been a large amount literature published about investigation spider species Chinese plantations from 1982 to 2020. Here, summarised and each regional recorded. To date, there were 535 40 families reported plantations.There 245 13 now being added checklist. A total 89 19 species, amongst them, Agelena labyrinthica, Allagelena difficilis, Neoscona theisi, Clubiona deletrix,...

Pederin, a group of antitumor compounds, is produced by an endosymbiotic bacterium Paederus fuscipes. Pederin content differed between male and female P. fuscipes, but the reason why these differences are maintained remains unexplored. Here, pederin-producing bacteria (PPB) infection rate in fuscipes was investigated. Furthermore, we assessed microbiota structure harbouring PPB sequenced transcriptome both sexes to shed light on genes interest. Of 625 analysed beetles (275 females, 350...

Ectropis grisescens (Lepidoptera, Geometridae) is one of the main leaf-eating pests in tea plantations China. In this study, complete mitochondrial genome species was sequenced and assembled. The total length E. 15,794 bp (GenBank accession No. MW337302). base composition 41.26% for A, 39.49% T, 7.92% G, 11.33% C. circular mitogenome contained 13 protein-coding genes, 2 ribosomal RNA 22 transfer genes. Phylogenetic analysis performed using genes 15 Geometridae an out-group Pieris melete...

Abstract As one of the most abundant predators insects in terrestrial ecosystems, spiders have long received much attention from agricultural scientists and ecologists. Do a certain controlling effect on main insect pests concern farmland ecosystems? Answering this question requires us to fully understand prey spectrum spiders. Next‐generation sequencing (NGS) has been successfully employed analyze spider spectra. However, high costs make it difficult various species with large samples given...

The Black Stork, Ciconia nigra belongs to family Ciconiidae, which is evaluated as Least Concern by IUCN. In this study, the complete mitochondrial genome of C. was first sequenced and characterized, 17,795 bp in length. mt-genome has tandem repeats 80 78 repeat units, AAACAAC AAACAAACAAC D-loop region. It notable that a single extra base "C" at position 174 inserted gene ND3. Bayesian inference, maximum likelihood methods were used construct phylogenetic trees based on 12 heavy-strand...

Abstract Pederin, a defensive toxin in Paederus fuscipes , is produced by an uncultured Gram‐negative symbiont, which establishes stable symbiotic relationship with female host before completion of metamorphosis. However, the transmission process pederin‐producing bacteria (PPB) P. at different life stages remains unknown. Herein, PPB population dynamics and transcriptome atlas for development (egg, first‐instar larva, second‐instar pupa, newly emerged male) were characterised. We found that...

The Arboreal Brown-toothed Shrew (Episoriculus macrurus) belongs to the family Soricidae, and distributes in China, Nepal, India, Myanma, Vietnam. In this study, complete mitochondrial genome sequence of E. macrurus was determined. mitogenome is 16,943 bp length. Phylogenetic trees were constructed by methods Bayesian inference maximum likelihood. results showed that Crocidurinae differentiated earlier than Soricinae. Episoriculus more close Nectogale or Neomys rather Sorex, Blarinella...