Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration biofilms that protect organisms from immune system clearance increased resistance phagocytosis antibiotics. Photodynamic treatment (PDT) has been proposed as an alternative approach for inactivation bacteria in biofilms. In this study, we have investigated effect photodynamic action toluidine blue O (TBO) on viability structure Staphylococcus epidermidis a...

The precise observation of the angle-frequency spectrum light filaments in water reveals a scenario incompatible with current models conical emission (CE). Its description terms linear X-wave modes leads us to understand filamentation dynamics requiring phase- and group-matched, Kerr-driven four-wave-mixing process that involves two highly localized pumps X waves. CE temporal splitting arise naturally as manifestations this process.

Optofluidic microsystems are key components towards lab-on-a-chip devices for manipulation and analysis of biological specimens. In particular, the integration optical tweezers (OT) in these allows stable sample trapping, while making available mechanical, chemical spectroscopic analyses.

We report on the fabrication by a femtosecond laser of an optofluidic device for optical trapping and stretching single cells.Versatility three-dimensional capabilities this technology provide straightforward extremely accurate alignment between fluidic components.Optical red blood cells are demonstrated, thus proving effectiveness proposed as monolithic stretcher.Our results pave way new class devices cell analysis, in which, taking advantage flexibility micromachining, it is possible to...

Abstract Three-dimensional (3D) particle focusing in microfluidics is a fundamental capability with wide range of applications, such as on-chip flow cytometry, where high-throughput analysis at the single-cell level performed. Currently, 3D achieved mainly devices complex layouts, additional sheath fluids, and pumping systems. In this work, we present compact microfluidic device capable high rates small footprint, without requirement external fields or lateral flows, but using only...

The main trend in optofluidics is currently towards full integration of the devices, thus improving automation, compactness and portability. In this respect femtosecond laser microfabrication a very powerful technology given its capability producing both optical waveguides microfluidic channels. current challenge biology possibility to perform bioassays at single cell level unravel hidden complexity nominally homogeneous populations. Here we report on new device implementing fully integrated...

A femtosecond laser fabricated microfluidic module for 3D hydrodynamic focusing allows confinement of fluids to a tight area in the channel center.

A high-throughput on-chip light sheet microscope allowing 3D reconstructions of large populations samples, even with standard microscopes, is presented.

Cell sorting based on mechanical properties is demonstrated using an optofluidic chip by extracting highly metastatic cells from a heterogeneous population.

We propose and demonstrate an on-chip optofluidic device allowing active oscillatory microrheological measurements with sub-μL sample volume, low cost high flexibility. Thanks to the use of this microrheometer it is possible measure viscoelastic properties complex fluids in frequency range 0.01-10 Hz at different temperatures. The system based on optical forces exerted a microbead by two counterpropagating infrared laser beams. core elements part, integrated waveguides modulator, are...

Single-cell analysis techniques are fundamental to study the heterogeneity of cellular populations, which is basis understand several biomedical mechanisms. Light-sheet fluorescence microscopy a powerful technique for obtaining high-resolution imaging individual cells, but complexity setup and sample mounting procedures limit its overall throughput. In our work, we present an optofluidic microscope-on-chip with integrated microlenses light-sheet shaping fluidic microchannel that allows...

By use of an imaging spectrometer we map the far-field ($\theta-\lambda$) spectra 200 fs optical pulses that have undergone beam collapse and filamentation in a Kerr medium. studying evolution with increasing input power using model based on stationary linear asymptotic wave modes, are able to trace consistent high-lighting interplay between conical emission, multiple pulse splitting other effects such as spatial chirp.

The authors present the design and optimization of an optofluidic monolithic chip, able to provide optical trapping controlled stretching single cells. chip is fabricated in a fused silica glass substrate by femtosecond laser micromachining which can produce both waveguides microfluidic channels with great accuracy. A new fabrication procedure adopted this work allows demonstration microchannels square cross-section, thus guaranteeing improved quality trapped cell images. Femtosecond emerges...

The combination of high power laser beams with microfluidic delivery cells is at the heart high-throughput, single-cell analysis and disease diagnosis an optical stretcher. So far, challenges arising from this have been addressed by externally aligning fibres glass capillaries, which has a limited potential for integration into lab-on-a-chip environments. Here we demonstrate successful production use monolithic chip stretching white blood cells, featuring channels waveguides directly written...

Abstract We realized an integrated microfluidic chip that allows measuring both optical deformability and acoustic compressibility on single cells, by stretching acoustophoresis experiments respectively. Additionally, we propose a measurement protocol evaluating the experimental apparatus parameters before performing cell-characterization experiments, including non-destructive method to characterize force distribution inside microchannel. The was used study important cell-mechanics in two...

Abstract Understanding of material behaviour at nanoscale under intense laser excitation is becoming critical for future application nanotechnologies. Nanograting formation by linearly polarised ultra-short pulses has been studied systematically in fused silica various pulse energies 3D printing/writing conditions, typically used the industrial fabrication optical elements. The period nanogratings revealed a dependence on orientation scanning direction. A tilt nanograting wave vector fixed...

Abstract We present a microscope on chip for automated imaging of Drosophila embryos by light sheet fluorescence microscopy. This integrated device, constituted both optical and microfluidic components, allows the automatic acquisition 3D stack images specimens diluted in liquid suspension. The device has been fully optimized to address challenges related under investigation. Indeed, thickness high ellipticity can degrade image quality. In this regard, fluidic optimization carried out...

Femtosecond laser micromachining (FLM) of fused silica allows for the realization three-dimensional embedded optical elements and microchannels with micrometric feature size. The performances these components are strongly affected by machined surface quality residual roughness. polishing 3D buried structures in glass was demonstrated using different thermal annealing processes, but precise control roughness obtained this technique is still missing. In work, we investigate how FLM irradiation...

Thanks to its unique properties, glass plays a fundamental role in science and technology, especially optics photonics. For instance, transparency has been exploited the last decades for efficiently guiding light optical fibers long distances, while versatility makes it perfect material different research fields, ranging from biology chemistry. On occasion of International Year Glass, we would like discuss powerful microfabrication technique devices this material: femtosecond laser...