A5107386860- Advanced MRI Techniques and Applications
- Advanced NMR Techniques and Applications
- Neuroscience and Neuropharmacology Research
- NMR spectroscopy and applications
- Amyotrophic Lateral Sclerosis Research
- Genetic Neurodegenerative Diseases
- Liver Disease Diagnosis and Treatment
- Diabetes Treatment and Management
- Atomic and Subatomic Physics Research
- Functional Brain Connectivity Studies
- Metabolomics and Mass Spectrometry Studies
- Neurogenetic and Muscular Disorders Research
- Prion Diseases and Protein Misfolding
- Solid-state spectroscopy and crystallography
- Asymmetric Hydrogenation and Catalysis
- Parkinson's Disease Mechanisms and Treatments
- Angiogenesis and VEGF in Cancer
- Medical Imaging Techniques and Applications
- Lung Cancer Treatments and Mutations
- Adipose Tissue and Metabolism
- Regulation of Appetite and Obesity
- Advanced Neuroimaging Techniques and Applications
- Catalysis and Oxidation Reactions
- Inflammatory Bowel Disease
- Phase Equilibria and Thermodynamics
Boehringer Ingelheim (Germany)
2013-2024
Target (Germany)
2013
Otto-von-Guericke University Magdeburg
2004-2007
University Hospital Magdeburg
2007
University of Bonn
2000-2003
University of California, Berkeley
2001
The development of therapeutics for ALS/MND is largely based on work in experimental animals carrying human SOD mutations. However, translation apparent therapeutic successes from vivo to the disease has proven difficult and a considerable amount financial resources been apparently wasted. Standard operating procedures (SOPs) preclinical animal research are urgently required. Such SOPs will help establish translational other neurological diseases within next few years. To identify challenges...
Tryptophan catabolites ("TRYCATs") produced by the kynurenine pathway (KP) may play a role in depression pathophysiology. Studies comparing TRYCATs levels depressed subjects and controls provided mixed findings. We examined association of with 1) presence Major Depressive Disorder (MDD), 2) depressive symptom profiles 3) inflammatory markers.The sample from Netherlands Study Depression Anxiety included participants current (n = 1100) or remitted 753) MDD DSM-IV diagnosis healthy 642). Plasma...
Linagliptin (TRADJENTA™) is a selective dipeptidyl peptidase-4 (DPP-4) inhibitor. DPP-4 inhibition attenuates insulin resistance and improves peripheral glucose utilization in humans. However, the effects of chronic on sensitivity are not known. The long-term treatment (3-4 weeks) with 3 mg/kg/day or 30 linagliptin liver fat content were determined diet-induced obese C57BL/6 mice. Chow-fed animals served as controls. activity was significantly inhibited (67-89%) by (P<0.001). Following an...
Summary Purpose: In patients with mesial temporal lobe epilepsy (MTLE) it remains an unresolved issue whether the interictal decrease in N‐acetyl aspartate (NAA) detected by proton magnetic resonance spectroscopy ( 1 H‐MRS) reflects epilepsy‐associated loss of hippocampal pyramidal neurons or metabolic dysfunction. Methods: To address this problem, we applied high‐resolution H‐MRS at 14.1 Tesla to measure metabolite concentrations ex vivo tissue slices from three subfields (CA1, CA3, dentate...
The molecular motor dynein and its associated regulatory subunit dynactin have been implicated in several neurodegenerative conditions of the basal ganglia, such as Huntington's disease (HD) Perry syndrome, an atypical Parkinson-like disease.This pathogenic role has largely postulated from existence mutations p150 Glued .However, is also able to act independently dynein, there currently no direct evidence linking ganglia degeneration.To provide evidence, we used here a mouse strain carrying...
Low dopamine D2/3 receptor availability in the nucleus accumbens shell is associated with highly impulsive behavior rats as measured by premature responses a cued attentional task. However, it unclear whether equally linked to intolerance for delayed rewards, related form of impulsivity. We investigated relationship between and impulsivity delay-discounting task where animals must choose immediate, small-magnitude rewards delayed, larger-magnitude rewards. Corticostriatal was stratified high...
Tyrosine kinase inhibitors (TKIs) have experienced a tremendous boost in the last decade, where more than 15 small molecule TKIs been approved by FDA. Unfortunately, despite their promising clinical successes, large portion of patients remain unresponsive to these targeted drugs. For non-small cell lung cancer (NSCLC), effectiveness is dependent on mutational status epidermal growth factor receptor (EGFR). The exon 19 deletion as well L858R point mutation lead excellent sensitivity such...
Abstract Aims Despite substantial improvements over the last three decades, heart failure (HF) remains associated with a poor prognosis. The sodium‐glucose co‐transporter‐2 inhibitor empagliflozin demonstrated significant reductions of HF hospitalization in patients independent presence or absence type 2 diabetes mellitus EMPEROR‐Reduced trial and cardiovascular mortality EMPA‐REG OUTCOME trial. To further elucidate mechanisms behind these positive outcomes, this study aims to determine...
The hydrogenation of internal alkynes using a [Cp*Ru(alkene)]+ complex leads to the formation (E)-alkenes. This ruthenium represents one few homogeneous catalysts that trans-hydrogenate directly and stereoselectively. We have studied its stereoselectivity by in situ PHIP-NMR spectroscopy (PHIP = para-hydrogen induced polarization). With this method initially formed products can be identified characterized even at very low concentrations conversions. Furthermore, their subsequent fate...
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive loss of motor neurons. Visualizing corresponding metabolic changes in the brain patients with ALS proton magnetic resonance spectroscopy ((1)H-MRS) may provide surrogate markers for an early detection, monitoring progression and evaluating treatment response. The primary objective our study was to evaluate whether modifications MR metabolite levels occur before clinical onset, these are...
Recently, Levitt and co-workers demonstrated that conserving the population of long-lasting nuclear singlet states in weak magnetic fields can lead to a preservation spin information over times substantially longer than governed by (high-field) spin-lattice relaxation time T1. Potential benefits prolonged for resonance imaging spectroscopy were pointed out, particularly when combined with parahydrogen induced polarization (PHIP) methodology. In this contribution, we demonstrate an increase...
Background: The hepatic lipidome of patients with early stages non-alcoholic fatty liver disease (NAFLD) has been fairly well-explored. However, studies on more progressive forms NAFLD, i.e., fibrosis, are limited. Materials and methods: Liver acids were determined in cholesteryl esters (CE), phospholipids (PL), triacylglycerols (TAG) by gas chromatography. Cross-sectional associations between biopsy-proven NAFLD fibrosis (n = 60) assessed using multivariable logistic regression models....
Summary: Purpose: After pilocarpine‐induced epilepsy in rats, volumetric magnetic resonance imaging (MRI) reveals significant morphologic changes functionally relevant structures of the brain. To relate structural to functional alteration, we studied correlation regional brain atrophy (e.g., hippocampus) with lesion‐induced learning deficits Morris water maze. Methods: MRI experiments were performed on an MR scanner at 4.7 Tesla. For analysis, various cerebral segmented horizontal and...
Manganese-enhanced magnetic resonance imaging (ME-MRI) was used to analyze the brain architecture in mice lacking functional presynaptic active zone protein Bassoon. Anatomical characterization revealed a significant increase total volume Bassoon mutants as compared with wild-type mice, which is mainly caused by changes cortex and hippocampus volume. The measured enlargement cortical coincides an altered Mn2+ distribution within layers visualized T1-weighted imaging. Two days after manganese...
Segregation structures of granular mixtures in rotating drums represent classical examples pattern formation material. We investigate the coarsening axial segregation patterns slurries a long horizontally cylinder. The dynamics and three-dimensional geometry are analyzed with optical methods nuclear magnetic resonance imaging. Previous studies have mainly considered global statistical features dynamics. In order to get insight into driving mechanisms for process, we focus on details...
Abstract Homogeneously catalyzed hydrogenations of unsaturated substrates with parahydrogen not only lead to strong polarization signals in 1 H NMR spectra, but also can give rise heteronuclear polarization, especially if the are carried out low magnetic fields. As a typical example, transfer from protons carbon nuclei during hydrogenation alkynes is outlined for several substrates. In systems containing easily accessible triple bonds, e.g. phenylethyne or 3,3‐dimethyl‐1‐butyne, occurs all...
Mice over-expressing the mutant human G93A-SOD1 are widely used as an animal model of amyotrophic lateral sclerosis (ALS). ALS is characterized by progressive degeneration motor neurons in cortex, brain stem and spinal cord. The underlying mechanisms for selective death still uncertain. To study factors that cause neuron or therapeutic approaches to delay progression disease, a method required monitor state under in-vivo conditions. Here, we demonstrate mice MRI signal intensities nucleus V,...
Abstract To evaluate cerebral metabolism and intergroup differences in closely matched patients with myotonic dystrophy type 2 (DM2, n = 15) 1 (DM1, 14), we performed H magnetic resonance spectroscopic (MRS) analyses of the occipital temporoparietal cortical regions as well subcortical frontal white matter. Relative to healthy subjects, concentration N ‐acetylaspartate was significantly reduced all tested brain both disease groups. In DM1 also observed a concomitant depletion creatine...
Abstract In recent years, magnetic resonance imaging (MRI) has emerged as a preferred tool for the diagnosis of amyotrophic lateral sclerosis (ALS) in humans. A widely used animal model human ALS is G93A‐superoxide dismutase 1 (G93A‐SOD1) transgenic mouse model. However, mechanisms selective degeneration motor neurons brainstem and spinal cord are still uncertain. our study, we applied MRI at 4.7 Tesla to non‐invasively evaluate pathological alterations this follow progression disease....
Background Nonalcoholic fatty liver disease (NAFLD) is rapidly increasing worldwide. It subdivided into nonalcoholic (NAFL) and the more aggressive form, steatohepatitis (NASH), which carries a higher risk of developing fibrosis cirrhosis. There currently no reliable non‐invasive method for differentiating NASH from NAFL. Purpose To investigate ability magnetic resonance imaging (MRI)‐based biomarkers to diagnose moderate as well assess their repeatability. Study Type Prospective. Subjects...
Mice lacking functional presynaptic active zone protein Bassoon are characterized by an enlarged cerebral cortex and altered cortical activation pattern. This morphological phenotype is associated with defined metabolic distortions as detected a metabonomic approach using high-field (14.1 T) high-resolution 1H-nuclear magnetic resonance spectroscopy (MRS) in conjunction statistical pattern recognition. Within the but not cerebellum, concentrations of N-acetyl aspartate, glutamine, glutamate...