A5089309785- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Advanced Nanomaterials in Catalysis
- Electrochemical sensors and biosensors
- Genetic factors in colorectal cancer
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Circular RNAs in diseases
- Neurogenetic and Muscular Disorders Research
- Environmental DNA in Biodiversity Studies
- Wastewater Treatment and Nitrogen Removal
- RNA and protein synthesis mechanisms
- Silicon Nanostructures and Photoluminescence
- MicroRNA in disease regulation
- Carbohydrate Chemistry and Synthesis
- Single-cell and spatial transcriptomics
- Child Nutrition and Feeding Issues
- Phytochemical compounds biological activities
- Biosensors and Analytical Detection
- Engineering Applied Research
- RNA Interference and Gene Delivery
- Synthesis of Organic Compounds
- Thermal Analysis in Power Transmission
State Key Laboratory of Digital Medical Engineering
2021-2025
Southeast University
2021-2025
Soochow University
2021-2023
Institute of Advanced Manufacturing Technology
2021
WuXi AppTec (China)
2019
Nanjing Agricultural University
2011
Shanghai Institute of Organic Chemistry
2010
Chinese Academy of Sciences
2010
Kaempferol 3-O-(3'',6''-di-O-E-p-coumaroyl)-β-D-glucopyranoside (1), an optimal metabolite of Scots pine seedlings for protection deep-lying tissue against damaging UV-B, represents a typical acylated flavonol 3-O-glycoside. This compound was synthesized the first time via two approaches. The approach, starting with kaempferol, featured formation 3-O-glycosidic linkage glycosyl bromide under conventional PTC conditions. In second 5,7,4'-tri-O-benzyl-kaempferol readily prepared from...
Abstract Clubfoot is a common birth defect characterized by inward posturing and rigid downward displacement of one or both feet. The etiology syndromic forms clubfoot varied the causes isolated are not well understood. A microduplication 2.2 Mb on chromosome 17q23.1q23.2 which includes T‐box 4 ( TBX4 ), hindlimb‐specific gene, 16 other genes was recently identified in 3 66 families reported as nonsyndromic clubfoot, but additional non‐foot malformations place them category. Our study...
RNA sequencing is an innovative technology to study transcriptomes in both biological and clinical research. However, specimens from patients undergoing surgical operations have a major challenge due sample degradation. This replicated the process of degradation by maintaining cells at room temperature achieve none, slight, middle, high levels with decreasing integrity numbers (RIN) approximately 9.8, 6.7, 4.4, 2.5, respectively. Next, differential expression mRNA long non-coding (lncRNA)...
Extrachromosomal circular DNA (eccDNA) has emerged as a potential biomarker for disease due to its stable closed structure. However, the diagnostic utility of eccDNA remains underexplored. In this study, we demonstrate that characteristics associated with genomic repetitive elements change in breast cancer patient tissues and plasma. These changes can serve signatures accurate classification. We profiled annotated repeat across genome plasma, aggregating each element superfamily subfamily...
Diffuse large B-cell lymphoma (DLBCL) is a common but difficult to treat type of non Hodgkin lymphoma. At present, platinum nanoparticles with enzyme mimicking activity - nanoenzymes have been well applied in the treatment DLBCL disease, molecular mechanism their still unclear and there certain degree drug resistance. Extrachromosomal circular DNA (eccDNA) elements shown be widely present eukaryotic genomes associated However, characteristics biological functions eccDNA are unclear. In this...
Abstract Reconstructing the full-length sequence of extrachromosomal circular DNA (eccDNA) from short sequencing reads has proved challenging given similarity eccDNAs and their corresponding linear DNAs. Previous methods were unable to achieve high-throughput detection eccDNAs. Herein, a novel algorithm was developed, called Full-Length eccDNA Detection (FLED), reconstruct based on strategy that combined rolling circle amplification nanopore long-reads technology. Seven human epithelial...
The occurrence of diseases displayed transcriptome alteration, including both coding and non-coding transcripts. third-generation sequencing (TGS) technologies allow for intensive comprehensive research the transcriptome. However, present standard TGS RNA method is unable to detect many non-polyadenylated [non-poly(A)] RNAs. To obtain more complete information, we presented a new approach by performing conventional poly(A) RNA-sequencing combined with non-poly(A) fraction which was tailed...
Extrachromosomal circular DNA (eccDNA) was discovered several decades ago, but little is known about its function. With the development of sequencing technology, library preparation methods have been developed to elucidate biogenesis and function eccDNA. However, different treatment certain biases that can lead their erroneous interpretation. To address these issues, we compared performance methods. Our investigation revealed utilization rolling-circle amplification (RCA) restriction enzyme...
Abstract The rate of pregnancy can be affected by many factors in assisted reproductive technology (ART), and one which is the quality embryos. Therefore, selecting embryos with high potential crucial for outcome. Fifteen spent blastocyst medium (SBM) samples were collected from 14 patients who received vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), seven high-grade eight low-grade Cell-free RNA (cf-RNA) profile SBM analyzed sequencing present study. It was found that...
An effective method to characterize full-length circRNA sequences from low-input RNA samples with rolling circular reverse transcription and nanopore sequencing.
A strain designated as XY-1,capable of degrading nitrobenzene efficiently,was isolated from the sludge in an chemical factory wastewater treatment plant.Strain XY-1 was identified preliminarily Pseudomonas sp.based on its physiological and biochemical characters result 16S rDNA homologue sequence analysis.Strain could grow with sole carbon source nitrogen source,and concentration 200 mg/L,the degradation rate 97% within 20 h.The optimal initial pH value temperature for by 8.0 30...
In southern power grid, four 500kV composite wall bushings used at a converter station was found significantly aging after 15 years' operation, such as chalking, cracks and low hydrophobicity. The housing material of these is liquid silicone rubber (LSR). order to extend the service life, out door insulation repaired by RTV. As internal failure, one forced be service. this paper, outer shed bushing evaluate characteristics LSR sheds long term operation. macroscopic were investigated using...
Photoelectrochemical (PEC) sensors have been explored as an efficient and facile way to detect glucose, but the detection limit is relatively high for sweat glucose monitoring well external bias enzymes are usually employed. In this work, self-driven enzyme-free PEC sensing realized by constructing CuO nanopariticles-modified TiO2 hierarchical nanotubes (CuO@TiO2 HNT). A sensitivity of 138.9 μA μM-1 cm-2 in a low concentration range (< 200 μM) 0.7 μM (Signal/Noise=3) achieved as-prepared...
Constructing novel hybrid nanostructure has become an effective strategy to enhance the performance of photoelectrochemical (PEC) biosensors. However, most H<sub>2</sub>O<sub>2</sub>-sensing photoelectrodes require enzyme modification, which limits working environment and sensing performance. Herein, burr-like CuO nanostructures are modified on entire surfaces ordered Si nanowires (SiNWs) by using a combination magnetron sputtering hydrothermal growth. The optimized CuO@SiNWs heterojunction...