A5064821049- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- Amyotrophic Lateral Sclerosis Research
- Circadian rhythm and melatonin
- Adipose Tissue and Metabolism
- Genetic Neurodegenerative Diseases
- Spaceflight effects on biology
- Prion Diseases and Protein Misfolding
- Biochemical Acid Research Studies
- Diet and metabolism studies
- Photosynthetic Processes and Mechanisms
- Birth, Development, and Health
- Neurological disorders and treatments
- Neurobiology and Insect Physiology Research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Microbial Metabolic Engineering and Bioproduction
- Sirtuins and Resveratrol in Medicine
- Vitamin C and Antioxidants Research
- Genetics and Neurodevelopmental Disorders
- Genomics, phytochemicals, and oxidative stress
- GABA and Rice Research
- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- Biochemical and Molecular Research
Cornell University
2021-2024
King Abdullah University of Science and Technology
2019-2021
Ithaca College
2021
Université de Montréal
2012-2017
Laboratoire de Biologie et Modélisation de la Cellule
2012-2014
L-lactate was long considered a glycolytic by-product but is now being recognized as signaling molecule involved in cell survival. In this manuscript, we report the role of stress resistance and survival mechanisms using neuroblastoma cells (SH-SY5Y) well C. elegans model. We observed that promotes cellular defense mechanisms, including Unfolded Protein Response (UPR) activation nuclear factor erythroid 2-related 2 (NRF2), by promoting mild Reactive Oxygen Species (ROS) burst. This increase...
Mutations in the DNA/RNA binding proteins TDP-43 and FUS are associated with Amyotrophic Lateral Sclerosis Frontotemporal Lobar Degeneration. Intracellular accumulations of wild type observed a growing number late-onset diseases suggesting that proteinopathies may contribute to multiple neurodegenerative diseases. To better understand mechanisms toxicity we have created transgenic Caenorhabditis elegans strains express full-length, untagged human worm's GABAergic motor neurons. Transgenic...
Glucose is a major energy source and key regulator of metabolism but excessive dietary glucose linked to several disorders including type 2 diabetes, obesity cardiac dysfunction. Dietary intake greatly influences organismal survival whether the effects nutritional status are transmitted offspring an unresolved question. Here we show that exposing Caenorhabditis elegans high concentrations in parental generation leads opposing negative on fecundity, while having protective against cellular...
Brain energy metabolism has been the object of intense research in recent years. Pioneering work identified different cell types involved production and use. Recent evidence demonstrated a key role L-Lactate brain metabolism, producing paradigm-shift our understanding neuronal metabolism. At center this shift, is identification central astrocytes, type glial cell, neuroenergetics. Thanks to their morphological characteristics, they are poised take up glucose from circulation deliver...
TDP-43 is a multifunctional nucleic acid binding protein linked to several neurodegenerative diseases including Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia. To learn more about the normal biological abnormal pathological role of this protein, we turned Caenorhabditis elegans its orthologue TDP-1. We report that TDP-1 functions in Insulin/IGF pathway regulate longevity oxidative stress response downstream from forkhead transcription factor DAF-16/FOXO3a. However, although...
Amyotrophic lateral sclerosis (ALS) is a heterogeneous disease with either sporadic or genetic origins characterized by the progressive degeneration of motor neurons. At cellular level, ALS neurons show protein misfolding and aggregation phenotypes. Transactive response DNA-binding 43 (TDP-43) has recently been shown to be associated ALS, but early pathophysiological deficits causing impairment in function are unknown. Here we used Caenorhabditis elegans expressing mutant TDP-43 A315T...
Nutrient availability influences an organism's life history with profound effects on metabolism and lifespan. The association between a healthy lifespan is incompletely understood, but central factor glucose metabolism. Although important cellular energy source, restriction associated extended in simple animals reduced incidence of age-dependent pathologies humans. We report here that enrichment delays mutant polyglutamine, TDP-43, FUS, amyloid-β toxicity Caenorhabditis elegans models...
The DNA/RNA binding proteins TAR DNA-binding protein 43 (TDP-43) and fused-in-sarcoma (FUS) are genetically linked to amyotrophic lateral sclerosis frontotemporal lobar dementia, while the inappropriate cytoplasmic accumulations of TDP-43 FUS observed in a growing number late-onset pathologies including spinocerebellar ataxia 3, Alzheimer's Huntington's diseases (HD). To investigate if contribute neurodegenerative phenotypes, we turned accessible Caenorhabditis elegans model polyglutamine...
Mitochondrial function is central to longevity and an imbalance in mitonuclear protein homeostasis activates a protective response called the mitochondrial unfolded (UPRmt). Toxic compounds damaging mitochondria trigger UPRmt, but at sublethal doses these insults extend lifespan simple animals like C. elegans. Mitochondria are main energy suppliers eukaryotes, it not known if diet influences UPRmt. High dietary glucose reduces worms, we show that high UPRmt protect against reduction. While...
Background: Polyglutamine expansion diseases are a group of hereditary neurodegenerative disorders that develop when CAG repeat in the causative genes unstably expanded above certain threshold. The trinucleotide repeats cause adult-onset such as Huntington's disease, dentatorubral-pallidoluysian atrophy, spinobulbar muscular atrophy and multiple forms spinocerebellar ataxia (SCA). most common dominantly inherited is type 3 (SCA3) also known Machado-Joseph disease (MJD), an autosomal...
Abstract Fatty acid desaturation is central to metazoan lipid metabolism and provides building blocks of membrane lipids precursors diverse signaling molecules. Nutritional conditions associated microbiota regulate desaturase expression, but the underlying mechanisms have remained unclear. Here, we show that endogenous microbiota-dependent small molecule signals promote via nuclear receptor NHR-49/PPARα in C. elegans . Untargeted metabolomics a β-oxidation mutant, acdh-11 , which expression...
Decisions made over long time scales, such as life cycle decisions, require coordinated interplay between sensory perception and sustained gene expression. The Caenorhabditis elegans dauer (or diapause) exit developmental decision requires integration of population density food availability to induce an all-or-nothing organismal-wide response, but the mechanism by which this occurs remains unknown. Here, we demonstrate how Amphid Single Cilium J (ASJ) chemosensory neurons, known be critical...
Nucleosides are essential cornerstones of life, and nucleoside derivatives synthetic analogues have important biomedical applications. Correspondingly, production non-canonical in animal model systems is particular interest. Here, we report the discovery diverse glucose-based nucleosides Caenorhabditis elegans related nematodes. Using a mass spectrometric screen based on all-ion fragmentation combination with total synthesis, show that C. selectively glucosylates series modified purines but...
ABSTRACT Fatty acid desaturation is central to metazoan lipid metabolism and provides building blocks of membrane lipids precursors diverse signaling molecules. Nutritional conditions associated microbiota regulate desaturase expression 1–4 , but the underlying mechanisms have remained unclear. Here, we show that endogenous microbiota-dependent small molecule signals promote via nuclear receptor NHR-49/PPARα in C. elegans . Untargeted metabolomics a β-oxidation mutant, acdh-11 which...
Summary L-lactate, for long considered a glycolytic end-product, is now recognized as an important energy substrate. Moreover, it appears that its role not limited to production but also signal neuroprotection and synaptic plasticity. Using model of neuroblastoma cells the nematode C. elegans we investigated cellular mechanisms underlying this protective L-lactate. We found L-lactate promotes mild Reactive Oxygen Species (ROS) induction translates into activation antioxidant defenses...