A5039088106- Genetic and phenotypic traits in livestock
- Parasitic Infections and Diagnostics
- Animal Genetics and Reproduction
- Genetic diversity and population structure
- Helminth infection and control
- Coccidia and coccidiosis research
- Genetic Mapping and Diversity in Plants and Animals
- Identification and Quantification in Food
- Sexual Differentiation and Disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- dental development and anomalies
- Animal Nutrition and Physiology
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Rabies epidemiology and control
- Prion Diseases and Protein Misfolding
- Oral microbiology and periodontitis research
- Viral gastroenteritis research and epidemiology
- Veterinary Oncology Research
- Vestibular and auditory disorders
- Animal Behavior and Welfare Studies
- Archaeological and Historical Studies
- Neurological diseases and metabolism
- Clostridium difficile and Clostridium perfringens research
Universidad de Zaragoza
2015-2024
Centro de Investigación y Tecnología Agroalimentaria de Aragón
2024
Universidad Complutense de Madrid
2015-2016
Universidad Católica Santo Domingo
2015
University of the Basque Country
2015
Hospital de Basurto
2015
Hospital Universitario Miguel Servet
1994-2015
Carleton University
2007
Ministry of the Interior
2007
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
1993
Abstract Preformed Fas ligand (FasL) and APO2 (APO2L)/TNF-related apoptosis-inducing (TRAIL) are stored in the cytoplasm of human Jurkat T cell line normal blasts. The rapid release these molecules their bioactive form is involved activation-induced death. In this study, we show by confocal microscopy that FasL APO2L/TRAIL mainly localized lysosomal-like compartments cells. We also immunoelectron inside cytoplasmic ∼500 nm diameter, with characteristics multivesicular bodies. Most share...
Limited molecular data exist on the prevalence and subtype distribution of Blastocystis spp., most prevalent parasite in human animal feces worldwide. A total 44 different subtypes (STs) are currently recognized based sequence small subunit ribosomal RNA (SSU-rRNA) gene. This is a study spp. hospitalized patients with gastrointestinal symptoms northern Spain. We analyzed 173 Blastocystis-positive by using nested PCR for detection, identification, phylogenetic analyses, genetic diversity...
Blastocystis sp. is known to be the most prevalent parasite in fecal samples of humans worldwide. In present report, a case–control study (1:9.89 (≈10)) was performed, by analyzing data from 3682 patients who attended public hospital northern area Spain showing gastrointestinal symptoms. Diagnosis performed human means optical microscopy. The prevalence with symptoms 9.18% (338/3682). Most sp.-infected tested negative for protozoa and helminths, were underweight foreign-born (26.4%), mainly...
Dominant black pigment synthesis in sheep is caused by alterations of the melanocortin‐1 receptor (MC1‐R) coding sequence. Using five bovine microsatellite markers we have mapped MC1‐R gene to chromosome 14, corresponding location other mammalian species. The existence two independent mutations, both causing an amino acid substitution, distantly related breeds sheep, support hypothesis that observed a mutual effect mutations. As similar mutations are found separately at locations dominant...
Previously, we reported the involvement of tyrosine phosphorylation in events that lead to ram sperm capacitation. In this study, carried out a comparative analysis localization tyrosine, serine and threonine phosphoproteins different functional stages spermatozoa (after swim-up procedure, vitro capacitation, ionophore-induced acrosome reaction) by immunofluorescence, immunocytochemistry confocal microscopy. Capacitation increased protein whereas induction reaction resulted significantly...
ABSTRACT A stock of 148 Cryptosporidium parvum DNA extracts from lambs and goat kids selected a previous study examining the occurrence species GP60 subtypes in diarrheic northeastern Spain was further characterized by multilocus fragment typing approach with six mini- microsatellite loci. Various degrees polymorphism were seen at all but MS5 locus, although markers exhibited two major alleles accounting for more than 75% isolates. total 56 (MLTs) (48 MLTs) (11 identified. Individual...
Species A rotavirus (RVA) is a major viral pathogen causing diarrhea in suckling piglets. Studies on its genetic heterogeneity have implications for vaccine efficacy the field. In this study, fecal samples (n = 866) from diarrheic piglets younger than 28 days were analyzed over two-year period (2018-2019). Samples submitted 426 farms located 36 provinces throughout Spain and tested using real-time PCR (qPCR) reverse transcription (RT-qPCR) five enteric pathogens. The individual prevalence...
Coxiella burnetii is an intracellular bacterium causing human Q fever and reproductive disorders in domestic ruminants. We analyzed the occurrence of C. co-infections with six other major pathogens abortion sheep (1242 cases) goat (371 flocks from Spain Portugal. After real-time PCR detection, were established by principal component cluster analysis that grouped cases based on joint presence/absence several microorganisms. Chlamydia abortus most common abortifacient agents approximately 75%...
In order to detect introgression of other Alectoris genus species into wild populations Spanish rufa, we studied a sample 93 red-legged partridges (supposed be A. rufa) captured in the island Majorca. A set 31 chukar (Alectoris chukar) from Cyprus and 33 (A. one farm were also provide suitable for comparison. Factorial correspondence analysis on microsatellite genotypes supported clear distinction birds Cyprus, whereas Majorca overlapped wide area. The existence mitochondrial DNA 16...
A collection of 140 Cryptosporidium parvum isolates previously analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP) and sequence analyses the small-subunit (SSU) rRNA 60-kDa glycoprotein (GP60) genes was further characterized multilocus typing six minisatellite (MSB MS5) microsatellite (ML1, ML2, TP14, 5B12) loci. Isolates were collected from diarrheic preweaned calves originating 61 dairy cattle farms in northern Spain. capillary electrophoresis-based tool combining three...
Detecting and quantifying the possible association between tegmen tympani (TT) status superior semicircular canal (SSC) pattern.Observational study.Study conducted in three tertiary Spanish hospitals.Nonselected consecutive patients of all ages (607 temporal bones).Thin-section multidetector row computed axial tomography (CAT scan) bones.Thickness SSC bone coverture adjacent to middle fossa, TT as a dichotomous variable: dehiscence (TTD) or integrity (TTI).The observed patterns were (3.79%),...
Summary We partially sequenced the mitochondrial hypervariable region 1 ( HVR1 ) in 60 goats belonging to six Spanish breeds. The analysis of these and previously published sequences reveals a weak phylogeographical structure Iberian Peninsula Individuals from single breed did not group into cluster. Furthermore, individuals different breeds often shared phylogenetic tree branches after UPGMA analysis. This could reflect non‐existence isolation because traditional seasonal pastoralism annual...
The intra-herd and intra-host genetic variability of 123 Cryptosporidium parvum isolates was investigated using a multilocus fragment typing approach with eleven variable-number tandem-repeat (VNTR) loci the GP60 gene. Isolates were collected from intensively farmed diarrheic pre-weaned calves originating 31 dairy farms in three adjoining regions northern Spain (País Vasco, Cantabria Asturias). tool demonstrated an acceptable typeability, 104/123 samples amplifying at all twelve loci. ML2,...
The dog was probably the first domesticated animal. Despite extensive archaeological and genetic investigations, origin evolution of extant dogs are still being debated. Dog breeds that have over time been selected for hunting share common ancestral traits. This study represents comprehensive attempt to survey at genomic mitochondrial level eight hound-like indigenous Mediterranean Basin determine if they ancient origins. Results from microsatellite analysis indicate all populations a low...
The cDNA for the bovine activin receptor type II (ACVR2) gene has been cloned and sequenced. It encodes a protein of 513 amino acids which is highly homologous (∼98 % identity) to human, mouse, rat proteins. Using PCR analysis × hamster somatic cell lines, ACVR2 was mapped syntenic group U17, localised chromosome 2. Comparative mapping shown that genes within U17 are also in on long arm human sheep 2, as well mouse 1. This represents an evolutionarily conserved mammalian chromosomal segment....
A multilocus fragment typing approach including eleven variable-number tandem-repeat (VNTR) loci and the GP60 gene was used to investigate intra-farm intra-host genetic diversity of Cryptosporidium parvum in sheep farms a confined area northeastern Spain. Genomic DNA samples 113 C. isolates from diarrheic pre-weaned lambs collected 49 meat-type were analyzed. Loci exhibited various degrees polymorphism, finding 7–9 alleles four most variable discriminatory markers (ML2, Cgd6_5400, Cgd6_3940,...