A5038329353- Veterinary medicine and infectious diseases
- Coccidia and coccidiosis research
- Parasitic Infections and Diagnostics
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Animal Nutrition and Physiology
- Infant Nutrition and Health
- Microbial infections and disease research
- Viral gastroenteritis research and epidemiology
- MicroRNA in disease regulation
- Animal Virus Infections Studies
- Salmonella and Campylobacter epidemiology
- Moringa oleifera research and applications
- Pharmaceutical studies and practices
- Escherichia coli research studies
- Information Retrieval and Data Mining
- Breastfeeding Practices and Influences
- Helicobacter pylori-related gastroenterology studies
- Gut microbiota and health
- Mycobacterium research and diagnosis
- Barrier Structure and Function Studies
- Pregnancy and Medication Impact
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Inflammatory Bowel Disease
University of California, San Diego
2024-2025
University of California San Diego Medical Center
2024
University of Veterinary Medicine Vienna
2016-2023
Evaluation of reference genes for expression studies in chickens and turkeys is very much limited unavailable various infectious models. In this study, eight candidate HMBS, HPRT1, TBP, VIM, TFRC, RPLP0, RPL13 RPS7 were evaluated by five different algorithms (GeNorm, NormFinder, BestKeeper©, delta CT, RefFinder) to assess their stability. order analyze a broad variation tissues, spleen, liver, caecum caecal tonsil aged specific pathogen free (SPF) layer commercial turkeys, uninfected or...
Abstract A recombinant fowl adenovirus (FAdV) fiber protein, derived from a FAdV-8a strain, was tested for its efficacy to protect chickens against inclusion body hepatitis (IBH). FAdV-E field isolates belonging both homotypic (FAdV-8a) and heterotypic (-8b) serotype were used as challenge. Mechanisms underlying fiber-induced protective immunity investigated by fiber-based ELISA, virus neutralization assays flow cytometry of peripheral blood mononuclear cells, monitoring the temporal...
Tumor necrosis factor (TNF) is a pleiotropic cytokine that plays critical role in the pathogenesis of immune-mediated diseases including inflammatory bowel disease (IBD). The stability its mRNA transcript, determined part by destabilizing sequences AAUU repeats (ARE) gene region, an important regulator tissue and systemic levels. A deletion ARE region resulted IBD arthritis mice pigs, supporting for human several arthritides. mutation same area mouse genome Genentech scientists (T.Y., M.K.)...
Abstract The protozoan parasite Histomonas meleagridis is the causative agent of re-emerging disease histomonosis chickens and turkeys. Due to parasite’s extracellular occurrence, a type-2 differentiation H. -specific T cells has been hypothesized. In contrast, recent study suggested that IFN-γ mRNA + are involved in protection against histomonosis. However, phenotype cytokine production profile still awaits elucidation. this work, clonal cultures virulent monoxenic strain were used for...
Avian pathogenic Escherichia coli (APEC) causes colibacillosis with different clinical manifestations. The disease is associated compromised animal welfare and results in substantial economic losses poultry production worldwide. So far, immunological mechanisms of protection against are not comprehensively resolved. Therefore, the present study aimed to use an ex vivo model applying chicken mononuclear cells stimulated by live inactivated APEC. For this purpose, 8-color flow cytometry panel...
The re-emerging disease histomonosis is caused by the protozoan parasite Histomonas meleagridis that affects chickens and turkeys. Previously, protection vaccination with in vitro attenuated H. has been demonstrated an involvement of T cells, potentially IFN-γ production, was hypothesized. However, comparative studies between turkeys on meleagridis-specific cells were not conducted yet. This work investigated production within CD4+, CD8α+ TCRγδ+ (chicken) or CD3ε+CD4−CD8α− (turkey) spleen...
Tight junctions (TJs) play a dominant role in gut barrier formation, therefore, resolving the structures of TJs any animal species is crucial but major importance fast growing broilers. They are regulated molecular composition, ultrastructure and function by intracellular proteins cytoskeleton. TJ classified according to their into barrier-forming, scaffolding pore-forming types with deductible consequences for permeability. In spite health its integrity limited studies have investigated...
Fowl adenovirus (FAdV)-induced diseases hepatitis-hydropericardium syndrome (HHS) and inclusion body hepatitis (IBH) have been affecting the poultry industry with increasing severity in last two decades. Recently, a subunit vaccine based on chimeric fiber protein epitopes from different fowl serotypes (named crecFib-4/11) has shown to confer simultaneous protection against both HHS IBH. However, underlying immune mechanisms chickens are still enigmatic, especially because of frequently...
The protozoan parasite Histomonas meleagridis is the causative agent of histomonosis in gallinaceous birds. In turkeys, disease can result high mortality due to severe inflammation and necrosis caecum liver, whereas chickens less severe. Recently, experimental vaccination was shown protect turkeys against but dynamics cellular immune response are not yet demonstrated. present work, different groups birds both species were vaccinated with attenuated, and/or infected virulent histomonads. Flow...
The present study investigated the expression of cytokines and cellular changes in chickens following vaccination with irradiated avian pathogenic Escherichia coli (APEC) and/or challenge. Four groups 11-week-old pullets, each consisting 16 birds were kept separately isolators before they sham inoculated (N), challenged only (C), vaccinated (V) or (V+C). Vaccination was performed using APEC applied via aerosol. For challenge, homologous strain administered intratracheally. Birds sacrificed...
Histomonosis is a parasitic disease of gallinaceous birds characterized by necrotic lesions in caecum and liver that usually turns fatal turkeys while it less severe chickens. Vaccination using vitro attenuated Histomonas meleagridis has been experimentally shown to confer protection against histomonosis. The protective mechanisms underpin the vaccine-induced immune response are not resolved so far. Therefore, actual study aimed evaluate location quantitative distribution patterns signature...
Histomonosis in turkeys and chickens is caused by the extracellular parasite Histomonas meleagridis, but outcome of disease varies depending on host species. So far, studies immune response against histomonosis focus mainly different traits adaptive system. Activation toll like receptors (TLR) leads to interplay between cells innate immunity with consequences B T cell clonal expansion. Therefore, present investigation focused interaction virulent and/or attenuated histomonads system at 4,...
Lay Summary Despite a known role for α4β7 and MAdCAM-1 the recruitment of antibody secreting cells to lactating mammary gland, vedolizumab which targets integrin did not lower breastmilk IgA in mothers with IBD receiving drug. It is likely that alternatively employ α4β1 arrest on VCAM-1 also expressed by gland.
ABSTRACTSalmonella (S.) Infantis is the most common serovar in broilers and broiler meat European Union. In field, fast-growing are reported to be more affected than slow-growing layer birds. The present study investigated infection dynamics immunological response of four chicken lines course a S. infection. Two commercial lines, Ross 308 Hubbard ISA-JA-757, two experimental specific pathogen free (SPF) layers broilers, were infected at 2 days age. Investigations focused on faecal shedding,...
Abstract Diarrheal diseases are a major cause of infant mortality worldwide. Breastfeeding helps prevent such by passive transfer maternal antibodies against gastrointestinal pathogens previously encountered the mother. The antibody secreting cells (ASC) that populate lactating mammary gland (LMG) educated in lymphoid tissues, thus they express integrin a4b7. To recruit these ASC, LMG expresses MAdCAM-1 and CCL28. Vedolizumab (vedo) blocks combinatorial epitope on a4b7, interfering with...
The population of the country is growing exponentially day by day. Therefore it very necessary for system to manage economy and also provide its citizens healthy food. backbone food industry depends on products supplied farmers country. It therefore essential help so that they can produce hefty amount grains production should be rapid but not any means false methods. This done with their past experiences grains. model completely focuses data analysis which notifies take appropriate steps...