A5012551211- RNA modifications and cancer
- Salivary Gland Disorders and Functions
- Cancer, Hypoxia, and Metabolism
- Advanced Proteomics Techniques and Applications
- Peptidase Inhibition and Analysis
- Galectins and Cancer Biology
- Cancer, Lipids, and Metabolism
- Oral Health Pathology and Treatment
- Salivary Gland Tumors Diagnosis and Treatment
- Oral microbiology and periodontitis research
- Glycosylation and Glycoproteins Research
- Bioinformatics and Genomic Networks
- Head and Neck Cancer Studies
- DNA Repair Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Genomics and Chromatin Dynamics
- Gene Regulatory Network Analysis
- Acute Myeloid Leukemia Research
- Chromosomal and Genetic Variations
- Innovative Microfluidic and Catalytic Techniques Innovation
- Ferroptosis and cancer prognosis
- Protease and Inhibitor Mechanisms
- Gene expression and cancer classification
- Single-cell and spatial transcriptomics
- Digital Imaging for Blood Diseases
Universidade Estadual de Campinas (UNICAMP)
2019-2025
Universität Hamburg
2023
Universidade Estadual Paulista (Unesp)
2022
Brazilian Center for Research in Energy and Materials
2019-2021
Brazilian Biosciences National Laboratory
2019-2021
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2021
Pontifícia Universidade Católica de Campinas
2021
Centro de Genética Clínica
2021
Weatherford College
2021
While altered protein glycosylation is regarded a trait of oral squamous cell carcinoma (OSCC), the heterogeneous and dynamic glycoproteome tumor tissues from OSCC patients remain unmapped. To this end, we here employ an integrated multi-omics approach comprising unbiased quantitative glycomics glycoproteomics applied to cohort resected primary with (n = 19) without 12) lymph node metastasis. all displayed relatively uniform N-glycome profiles suggesting overall stable global N-glycosylation...
Single-cell proteomics (SCP) has advanced considerably but still is largely limited to homogeneous populations and distant from clinical applicability. We present an SCP workflow for assessing the cellular heterogeneity in saliva tear fluid. Initially, benchmarks were established using a standard HeLa digestion curve, resulting more than 5,463 protein groups (PGs) at 50 pg. For single cells, was improved minimize contamination increase quantitative performance, reaching maximum of 3,785 PGs...
Understanding the sublethal effects of insecticides on non-target insects is essential for integrated pest management (IPM). This study aimed to evaluate differentially expressed genes (DEGs) in testes Ceraeochrysa claveri adults exposed pyriproxyfen during larval stage. Larvae (0–12 h) were fed Diatraea saccharalis eggs treated with (50 and 100 mg a.i. L−1) 10 days. After this exposure, larvae untreated until pupation. The from extracted RNA extraction, library construction, sequencing....
Protease activity has been associated with pathological processes that can lead to cancer development and progression. However, understanding the unbalance in proteolysis is challenging because changes occur simultaneously at protease, their inhibitor, substrate levels. Here, we present a pipeline combines peptidomics, proteomics, peptidase predictions for studying proteolytic events saliva of 79 patients association oral squamous cell carcinoma (OSCC) prognosis. Our findings revealed...
•Proteomic, miRNA, metabolomic, and lipidomic profiles were mapped in oral cancer EVs.•The molecular profile of EVs was associated with the lymph node metastatic phenotype.•A multi-omics integrative analysis revealed 11 highly connected 'hub proteins.'•'Hub proteins' from are candidates as prognostic markers cancer. Oral squamous cell carcinoma (OSCC) has high mortality rates that largely metastasis. However, mechanisms drive OSCC metastasis unknown. Extracellular vesicles (EVs)...
Abstract Motivation The reconstruction of small key regulatory networks that explain the differences in development cell (sub)types from single-cell RNA sequencing is a yet unresolved computational problem. Results To this end, we have developed SCANet, an all-in-one package for profiling covers whole differential mechanotyping workflow, inference trait/cell-type-specific gene co-expression modules, driver detection, and transcriptional network to mechanistic drug repurposing candidate...
Most complex diseases, including cancer and non-malignant diseases like asthma, have distinct molecular subtypes that require clinical approaches. However, existing computational patient stratification methods been benchmarked almost exclusively on omics data only perform well when mutually exclusive can be characterized by many biomarkers. Here, we contribute with a massive evaluation attempt, quantitatively exploring the power of 22 unsupervised using both, simulated real transcriptome...
The poor prognosis of head and neck cancer (HNC) is associated with metastasis within the lymph nodes (LNs). Herein, proteome 140 multisite samples from a 59-HNC patient cohort, including primary matched LN-negative or -positive tissues, saliva, blood cells, reveals insights into biology potential biomarkers that may assist in clinical decision-making. Protein profiles are strictly immune modulation across datasets, this provides basis for investigating markers metastasis. LN metastatic...
Diverse proteomics-based strategies have been applied to saliva quantitatively identify diagnostic and prognostic targets for oral cancer. Considering that these may be regulated by events do not imply variation in protein abundance levels, we hypothesized changes conformation can associated with diagnosis prognosis, revealing biological processes novel of clinical relevance. For this, employed limited proteolysis-mass spectrometry samples explore structural alterations, comparing the...
Abstract Diverse proteomics-based strategies have been applied to saliva quantitatively identify diagnostic and prognostic targets for oral cancer. Considering that these potential factors may be regulated by events do not imply variation in protein abundance levels, we investigated the hypothesis changes conformation can associated with diagnosis prognosis, revealing biological processes novel of clinical relevance. For this, employed limited proteolysis-mass spectrometry samples explore...
SUMMARY The poor prognosis of head and neck cancer (HNC) is associated with the presence metastasis within lymph nodes (LNs). Herein, proteome 140 multisite samples from a 59-HNC patient cohort, including primary matched LN-negative or -positive tissues, saliva, blood cells, reveals insights into biology potential biomarkers that may assist in clinical decision making. Protein profiles are strictly immune modulation across datasets, this provides basis for investigating markers metastasis....
Abstract While altered protein glycosylation is regarded a trait of oral squamous cell carcinoma (OSCC), its heterogeneous glycoproteome and dynamics with disease progression remain unmapped. To this end, we here employ an integrated multi-omics approach comprising unbiased quantitative glycomics glycoproteomics applied to valuable cohort resected tumour tissues from OSCC patients (n = 19) without 12) lymph node metastasis. all displayed uniform N -glycome profiles suggesting relatively...