A5072787193- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- Liver Disease and Transplantation
- Organ Donation and Transplantation
- Xenotransplantation and immune response
- Pancreatic function and diabetes
- Renal Diseases and Glomerulopathies
- Transplantation: Methods and Outcomes
- Liver Disease Diagnosis and Treatment
- Cannabis and Cannabinoid Research
- Pregnancy and Medication Impact
- Liver physiology and pathology
- Organ and Tissue Transplantation Research
- Diabetes Management and Research
- Renal and Vascular Pathologies
- Cytomegalovirus and herpesvirus research
- Pharmacological Effects and Toxicity Studies
- Diabetes and associated disorders
- Vascular Procedures and Complications
- Polyomavirus and related diseases
- Central Venous Catheters and Hemodialysis
- Dialysis and Renal Disease Management
- Cardiac Ischemia and Reperfusion
- Complement system in diseases
- Parvovirus B19 Infection Studies
University of Arizona
2016-2024
Loughborough University
2022-2023
Zoological Society of London
2022
Banner - University Medical Center Tucson
2018
East Carolina University
2012-2014
Gheskio Centers
2014
University of Chicago
2004-2010
NHS Blood and Transplant
2004-2009
Ohio University
2004
Cancer Research Center
2004
To date, limited information is available describing the incidence and impact of de novo donor-specific anti-human leukocyte antigen (HLA) antibodies (dnDSA) in primary renal transplant patient. This report details dnDSA actual 3-year post-dnDSA graft outcomes.The study includes 189 consecutive nonsensitized, non-HLA-identical patients who received a kidney between March 1999 2006. Protocol testing for DSA via LABScreen single beads (One Lambda) was done before transplantation at 1, 3, 6, 9,...
In 1990, more than 27,000 patients died of liver failure in the United States1. Perfusion through a from another person or member species, such as monkey, outside body was used past to stabilize condition some with acute subacute failure2–5. Few survived, however, and this approach superseded by orthotopic transplantation6. Liver transplantation is associated higher mortality for fulminant hepatic chronic disease, however. Patients often have multiorgan . . .
Background. Prospective randomized trials have shown a reduced rate of acute rejection (AR) in mycophenolate mofetil-treated kidney transplant recipients. We hypothesized that this increased protection from AR could allow successful prednisone (P) withdrawal cyclosporine/mycophenolate mofetil/P-treated Methods. A multicenter, prospective, randomized, double-blind trial P at 3 months posttransplant was initiated. Entry criteria were: primary transplant, adult, no by 90 days, mofetil dose ≥2...
Long-term success in xenotransplantation is currently hampered by acute vascular rejection. The inciting cause of rejection not yet known; however, a variety observations suggest that the humoral immune response recipient against donor may be involved pathogenesis this process. Using pig-to-baboon heterotopic cardiac transplant model, we examined role antibodies development After transplantation into baboons, hearts from transgenic pigs expressing human decay-accelerating factor and CD59...
To the Editor: Polyomavirus type BK nephropathy is an aggressively destructive disease occurring in up to 8 percent of patients with renal allografts,1,2 rates graft loss within one year 30 65 percent.3,4 There no therapy proven efficacy. Leflunomide (Arava), approved for treatment rheumatoid arthritis, immunosuppressive drug, yet its active metabolite, A77 1726, has substantial antiviral activity vitro and animals.5 From July 2001 April 2004, we used leflunomide as initial 17 biopsy-proven...
Background. Polyoma BK virus produces an aggressively destructive nephropathy in approximately 3% to 8% of renal allografts, is associated with graft loss within one year 35% 67% those infected and there no therapy proven efficacy. Leflunomide immune suppressive drug anti viral activity vitro animals. Methods. We treated twenty-six patients biopsy NK (BKN) either leflunomide alone (n=17) or plus a course cidofovir (n=9) followed them for six forty months. was dosed targeted blood level...
Reports have associated non-HLA antibodies, specifically those against angiotensin II type-1 receptor (AT1R), with antibody-mediated kidney graft rejection. However, association of anti-AT1R failure had not been demonstrated. We tested and donor-specific HLA antibodies (DSA) in pre- posttransplant sera from 351 consecutive recipients: 134 biopsy-proven rejection and/or lesions (abnormal biopsy group [ABG]) 217 control (CG) patients. The ABG's rate was significantly higher than the CG's (18%...
Immunoglobulins regulate the complement system by activating on foreign surfaces and diverting reactive proteins away from autologous cell surfaces. Based this model, we explored ability of Ig to balance activation versus control in a pig-to-primate cardiac xenotransplantation model which binding xenoreactive antibodies recipient graft blood vessels cause hyperacute rejection. Human IgG added human serum caused dose-dependent decrease deposition iC3b, cytotoxicity, heparan sulfate release...
Anti-HLA-DQ antibodies are the predominant HLA class II donor-specific (DSAs) after transplantation. Recently, de novo DQ DSA has been associated with worse allograft outcomes. The aim of this study was to determine further complement-binding characteristics most harmful DSA.Single-antigen bead technology used screen 284 primary kidney transplant recipients for presence posttransplantation DSA. Peak sera 34 only and 20 plus other DSAs were analyzed by a modified single-antigen assay using...
Hepatic damage following ischemia-reperfusion injury involves polymorphonuclear leukocytes (PMN) and platelet sequestration, however the mechanisms of adhesion remain elusive. In this study, using gene-targeted deficient mice, we evaluated P-selectin its contribution to PMN in hepatic damage. an vivo warm ischemia model, was assessed by serum transaminase levels, survival, histological analysis, sequestration immunostaining. Serum levels were strikingly reduced (by up threefold) particularly...
A specific gas chromatographic assay has been developed for measurement of valproic acid (VPA) and its major conjugated metabolites. In rats given single intravenous doses, the decline in blood concentration VPA was dose-dependent followed first-order kinetics only at lowest dose. The time required maximum VPA, after completion brief distribution phase, to by 50% 11.7, 41 125 min doses 15, 150 600 mg NaVPA per kg, respectively. secondary increase drug concentration, abolished exteriorization...
Background1-year data from this trial showed the noninferiority of a novel once-daily extended-release tacrolimus (LCPT; Envarsus XR) to immediate-release (IR-Tac) twice daily after kidney transplantation.Study DesignFinal 24-month analysis 2-armed, parallel-group, randomized, double-blind, double-dummy, multicenter, phase 3 trial.Setting & Participants543 de novo recipients randomly assigned LCPT (n = 268) or IR-Tac 275); 507 (93.4%) completed study.InterventionLCPT tablets once at 0.17...
With standard IgG donor-specific anti-HLA antibody (DSA) testing, it is unclear which immunoglobulin-G (IgG) DSA positive patients will fail. We looked further into the immune response by studying immunoglobulin-M (IgM) and subclass 3 (IgG3) to determine if these identify at highest risk for allograft loss.In 189 consecutively transplanted primary renal recipients, sera were collected sequentially pre- posttransplant. Of 189, 179 had available retrospectively test IgG, IgM, IgG3 antibodies...
This study assessed the efficacy and safety of anti-CD40 monoclonal antibody bleselumab (ASKP1240) in de novo kidney transplant recipients over 36 months posttransplant. Transplant were randomized (1:1:1) to standard care (SoC: 0.1 mg/kg per day immediate-release tacrolimus [IR-TAC]; target minimum blood concentration [C<sub>trough</sub>] 4-11 ng/mL plus 1 g mycophenolate mofetil [MMF] twice daily) or (200 mg on days 0/7/14/28/42/56/70/90, monthly thereafter) either MMF IR-TAC (0.1 day;...
A major question in xenotransplantation is the nature of humoral response that would occur following transplantation a xenogeneic organ into an immunosuppressed recipient as such could mediate delayed types injury to graft. To begin address this issue we characterized changes properties xenoreactive antibodies occurring patients exposed porcine organs under conditions simulating transplantation. In two whose blood had been cross-perfused through livers treatment for hepatic failure, titer...
No AccessJournal of UrologyInvestigative Urology1 Dec 2004RENAL TOLERANCE TO PROLONGED WARM ISCHEMIA TIME IN A LAPAROSCOPIC VERSUS OPEN SURGERY PORCINE MODEL BRETT A. LAVEN, MARCELO ORVIETO, MARC S. CHUANG, CHAD R. RITCH, PATRICK MURRAY, ROBERT C. HARLAND, SHARON INMAN, CHARLES B. BRENDLER, and ARIEH L. SHALHAV LAVENBRETT LAVEN , ORVIETOMARCELO ORVIETO CHUANGMARC CHUANG RITCHCHAD RITCH MURRAYPATRICK MURRAY HARLANDROBERT HARLAND INMANSHARON INMAN BRENDLERCHARLES BRENDLER SHALHAVARIEH View All...
Islet allotransplantation in the United States (US) is facing an imminent demise. Despite nearly three decades of progress field, archaic regulatory framework has stymied US clinical practice. Current regulations do not reflect state-of-the-art or technical practices. In US, islets are considered biologic drugs and "more than minimally manipulated" human cell tissue products (HCT/Ps). contrast, across world, appropriately defined as "minimally manipulated tissue" regulated a drug, which led...
Leukocytes recruited during ischemia-reperfusion to the liver are important mediators of injury. However, mechanisms leukocyte adhesion and role receptors in hepatic vasculature remain elusive. L-selectin may critically contribute injury, priming for later action intercellular molecule-1 (ICAM-1). Paired experiments were performed using mutant mice (L-selectin -/-, ICAM-1 L-selectin/ICAM-1 -/-) wild-type (C57BL/6) investigate ischemic liver. Leukocyte infiltration assessed histologically....
Hyperacute rejection of renal and cardiac xenografts is initiated by the reaction recipient natural antibodies complement with endothelial cell antigens donor organ. The liver thought to be less susceptible this form rejection; however, mechanisms underlying its decreased susceptibility are not known. We investigated organ injury occurring in porcine livers perfused blood from 4 human subjects fulminant hepatic failure. Nine were via an extracorporeal circuit order provide temporary...
In October 2005, the United Network for Organ Sharing (UNOS) implemented a revised allocation policy requiring that renal allografts from young deceased donors (DDs) (<35 years old) be offered preferentially to pediatric patients (<18 old). this study, we compare pre- and postpolicy quarterly transplant statistics 2000 2008. The mean number of transplants with DDs increased after implementation 62.8 133 per quarter (p < 0.001), reflecting change in proportion all during study period 0.33...
Objective The authors evaluated the morbidity, mortality, and quality of life after pancreatic debridement for necrosis compared these values to those elective medical surgical management chronic pancreatitis. Summary Background Data Quality has received little attention. Although other surgery been is thought be good, patients with can labor intensive require extraordinary resources. Therefore, further evaluation achieved treatment appropriate. Methods Forty (group 1) underwent operative...
Immunohistochemical detection of the C4d complement product along peritubular capillaries (PC) may indicate humoral rejection renal allografts. We examined frequency PC expression in renal-allograft biopsies with acute (AR) arising more than 6 months after transplantation and impact this finding.C4d was detected by immunoperoxidase 2-micron paraffin sections consecutive obtained over a 3-year period. The extent classified as diffuse (> or =50% C4d+), focal (<50% negative (C4d-). Clinical...