A5060687430- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Probiotics and Fermented Foods
- Gut microbiota and health
- Renal Transplantation Outcomes and Treatments
- Helicobacter pylori-related gastroenterology studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Clostridium difficile and Clostridium perfringens research
- Biochemical and Molecular Research
- Cytomegalovirus and herpesvirus research
- Genomics and Rare Diseases
Instituto de Investigación Biosanitaria de Granada
2015-2019
Red de Investigación en Sida
2019
Complejo Hospitalario Universitario de Granada
2015-2018
The beneficial effects of probiotics on immune-based pathologies such as inflammatory bowel disease (IBD) have been well reported. However, their exact mechanisms not fully elucidated. Few studies focused the impact composition colonic microbiota. aim present study was to correlate intestinal anti-inflammatory activity probiotic Escherichia coli Nissle 1917 in dextran sodium sulphate (DSS) model mouse colitis with changes induced microbiota populations. prevented DSS-induced damage,...
The colon microbiota plays a crucial role in human gastrointestinal health. Current attempts to manipulate the composition are aimed at finding remedies for various diseases. We have recently described immunomodulatory effects of three probiotic strains (Lactobacillus rhamnosus CNCM I-4036, Lactobacillus paracasei I-4034, and Bifidobacterium breve I-4035). goal present study was analyze compositions fecal healthy adults who received one these using high-throughput 16S ribosomal RNA gene...
We aimed to evaluate the correct assignment of HCV genotypes by three commercial methods—Trugene genotyping kit (Siemens), VERSANT Genotype 2.0 assay and Real-Time genotype II (Abbott)—compared NS5B sequencing. studied 327 clinical samples that carried representative most frequent geno/subtypes in Spain. After genotyping, sequencing a 367 bp fragment gene was used assign genotypes. Major discrepancies were defined, e.g. differences assigned one methods sequencing, including misclassification...
Current HCV genotyping methods may have some limitations in detecting mixed infections. We aimed to determine the accuracy of and detection mixed-genotype infections using Abbott-RealTime Genotype II assay (Abbott-RT-PCR) comparison with a Roche-Next Generation Sequencing (Roche-NGS). Plasma samples collected from 139 HCV-infected patients tested Abbott-RT-PCR, 114 single genotype (GT) 25 GTs were genotyped Roche-NGS. Roche-NGS confirmed all obtained Abbott-RT-PCR. One case Abbott GT 4 was...
The HIV-1 CRF19_cpx genetic form has been recently associated with greater pathogenicity. We used CoRIS, a national cohort of 31 reference hospitals in Spain, to investigate the current epidemiological situation this variant Spain.We analysed 4734 naive HIV-1-positive patients diagnosed during 2007-15 period an available pol gene sequence CoRIS resistance database. was ascribed through REGA3.0 and confirmed by phylogenetic analysis. presence transmission clusters maximum likelihood [with...
We aimed to evaluate the correct assignment of HCV genotype/subtypes 1a and 1b by cobas® genotyping (GT) assay (Roche Molecular Diagnostics) compared with nonstructural protein 5B (NS5B) sequencing.Clinical samples from 153 patients submitted for were studied. After GT, sequencing a 387 bp fragment in NS5B gene phylogenetic analysis was employed compare results. Major discrepancies defined as differences assigned genotype GT (including 1 subtypes misclassification).Overall agreement between...
In our study, we have hypothesized that proviral DNA may show the history of mutations emerged at previous failures to a Raltegravir containing regimen, in patients who are currently undetectable and candidates simplification Dolutegravir order decide on once day or twice dosing. We performed pilot, observational, retrospective, non interventional including 7 infected by HIV-1, all with failure RAL were successfully salvaged had reached viral suppression. A genotypic Integrase region study...