A5049077102- Cancer-related gene regulation
- Chromatin Remodeling and Cancer
- Epigenetics and DNA Methylation
- Cancer Mechanisms and Therapy
- MicroRNA in disease regulation
- Advanced Manufacturing and Logistics Optimization
- Peptidase Inhibition and Analysis
- Sesame and Sesamin Research
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Trace Elements in Health
- Histone Deacetylase Inhibitors Research
- Scheduling and Optimization Algorithms
- Protein Hydrolysis and Bioactive Peptides
- Nuclear Receptors and Signaling
- Manufacturing Process and Optimization
- Bone Metabolism and Diseases
- RNA modifications and cancer
- Cancer-related Molecular Pathways
Nanjing University
2019-2024
Nanjing Drum Tower Hospital
2021-2023
Nanjing University of Chinese Medicine
2018
Aberrant changes in epigenetic mechanisms such as histone modifications play an important role cancer progression. PRMT1 which triggers asymmetric dimethylation of H4 on arginine 3 (H4R3me2a) is upregulated human colorectal (CRC) and essential for cell proliferation. However, how this dysregulated modification might contribute to malignant transitions CRC remains poorly understood.In study, we integrated biochemical assays including protein interaction studies chromatin immunoprecipitation...
The proto-oncogene c-Myc regulates multiple biological processes mainly through selectively activating gene expression. However, the mechanisms underlying c-Myc-mediated repression in context of cancer remain less clear. This study aimed to clarify role PRMT5 transcriptional target genes gastric cancer. Methods: Immunohistochemistry was used evaluate expression PRMT5, and patients. interaction assessed by immunofluorescence, co-immunoprecipitation GST pull-down assays. Bioinformatics...
Aurora kinase B (AURKB) triggers the phosphorylation of serine 10 on histone H3 (H3S10ph), which is important for chromosome condensation and cytokinesis during mitosis in mammals.However, how exactly AURKB controls cell cycle contributes to tumorigenesis as an oncoprotein under pathological conditions remains largely unknown.Here, we report that promotes gastric cancer proliferation vitro vivo.Silencing expression inhibits arrests G2/M phase.We demonstrate cyclin D1 (CCND1) a direct...
Genomic studies have demonstrated a high frequency of genetic alterations in components the SWI/SNF complex including core subunit SMARCA4. However, mechanisms tumorigenesis driven by SMARCA4 mutations, particularly colorectal cancer (CRC), remain largely unknown. In this study, we identified specific, hotspot mutation (c. 3721C>T) which results conversion from arginine to tryptophan at residue 1157 (R1157W) human CRC tissues associated with higher-grade tumors and controls progression....
Endometrial cancer (EC) is the most common gynecological malignancy worldwide. However, molecular mechanisms underlying EC progression are still largely unknown, and chemotherapeutic options for patients currently very limited. In this study, we found that histone methyltransferase EZH2 DNA DNMT3B were upregulated in samples from patients, promoted cell proliferation as evidenced by assays of viability, cycle, colony formation. Mechanistically, epigenetically repressing TCF3, a direct...
Abstract Background Lysine methyltransferase 2D (KMT2D) mediates mono‐methylation of histone H3 lysine 4 (H3K4me1) in mammals. H3K4me1 mark is involved establishing an active chromatin structure to promote gene transcription. However, the precise molecular mechanism underlying KMT2D‐mediated modulates expression triple‐negative breast cancer (TNBC) progression unresolved. Methods and Results We recognized Y‐box‐binding protein 1 (YBX1) as a “reader” mark, point mutation YBX1 (E121A)...
Puerarin is an isoflavonoid phytoestrogen extracted from the root of Radix Pueraria, has attracted increasing attention because its beneficial effects on anti-osteoporosis, but molecular mechanisms underlying actions osteoblasts are not fully understood. The current study aimed to investigate effect puerarin MC3T3-E1 osteoblastic cells proliferation, differentiation and mineralization, in vitro mechanisms. results indicated that significantly promoted enhanced alkaline phosphatase activity...
Puerarin is a bioactive substance extracted from Pueraria lobata. It known to promote the viability, differentiation and mineralization of osteoblasts. However, molecular mechanisms involved in these activities are not well understood. The present study was conducted with aim elucidating effect puerarin on osteoblasts explore underlying mechanism. CCK-8 analysis showed that (0.1, 1 10 µM) promoted viability osteoblastic MC3T3-E1 cells, µM exhibiting strongest effect. Moreover, significantly...
In this paper, we proposed a fast-automated scheduling method for the final assembly workshop based on Genetic Algorithm. This belongs to technical field of production planning management involving application genetic algorithm and computer simulation in advanced scheduling. Comprehensively, take into account realistic factors including order priority, lines workload balance time-consuming design our three-level scheme evaluation systems. Moreover, priority is decided by Decision Tree model...
Abstract Background: Genomic studies have demonstrated a high frequency of genetic alterations in components the SWI/SNF complex including core subunit SMARCA4. However, mechanisms tumorigenesis driven by SMARCA4 mutations, particularly colorectal cancer (CRC), remain largely unknown. Methods: To reveal role R1157W mutation CRC cells and its consequences on progression, we used multiple approaches techniques. These include DNA sequencing, CRISPR/Cas9 genome editing system, biochemical assays...