A5032847508- Mitochondrial Function and Pathology
- Career Development and Diversity
- ATP Synthase and ATPases Research
- Mentoring and Academic Development
- Metabolism and Genetic Disorders
- Adipose Tissue and Metabolism
- Higher Education Research Studies
- Autophagy in Disease and Therapy
- Ion channel regulation and function
- Diversity and Career in Medicine
- Endoplasmic Reticulum Stress and Disease
- Muscle Physiology and Disorders
- Metabolomics and Mass Spectrometry Studies
- Neuroscience and Neural Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Genetic Neurodegenerative Diseases
- Genetics, Bioinformatics, and Biomedical Research
- Ubiquitin and proteasome pathways
- Education, Achievement, and Giftedness
- Lipid metabolism and biosynthesis
- Neurobiology and Insect Physiology Research
- Science Education and Pedagogy
- Infectious Aortic and Vascular Conditions
- Advanced Electron Microscopy Techniques and Applications
- Biomedical and Engineering Education
University of Iowa
2018-2025
Vanderbilt University
2021-2024
Universidade Federal do Estado do Rio de Janeiro
2023
Rush University
2021
Universidad Nacional Autónoma de México
2016-2020
Universidad Nacional de Asunción
2019
Center for Research and Advanced Studies of the National Polytechnic Institute
2012-2017
Instituto Politécnico Nacional
2012-2017
Stanford Health Care
2013
Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria endoplasmic reticulum (ER) are organelles particular interest those investigating metabolic disorders. A straightforward method for quantifying characterizing aspects these would be a useful tool. In this protocol, we outline how accurately assess the morphology important structures using open source software ImageJ,...
Abstract Various intracellular degradation organelles, including autophagosomes, lysosomes, and endosomes, work in tandem to perform autophagy, which is crucial for cellular homeostasis. Altered autophagy contributes the pathophysiology of various diseases, cancers metabolic diseases. This paper aims describe an approach reproducibly identify distinguish subcellular structures involved macroautophagy. Methods are provided that help avoid common pitfalls. How between lipid droplets,...
Mentoring is a developmental experience intended to increase the willingness learn and establish credibility while building positive relationships through networking. In this commentary, we focus on intentional mentoring for underrepresented mentees, including individuals that belong minority racial, ethnic gender identity groups in Science, Technology, Engineering, Mathematics Medicine (STEMM) fields. Intentional superpower action necessary developing harmony comprehending purpose value of...
Mitochondria respond to metabolic demands of the cell and incremental damage, in part, through dynamic structural changes that include fission (fragmentation), fusion (merging distinct mitochondria), autophagic degradation (mitophagy), biogenic interactions with endoplasmic reticulum (ER). High resolution study mitochondrial functional relationships requires rapid preservation specimens reduce technical artifacts coupled quantitative assessment architecture. A practical approach for...
This article shows how mitochondria in murine cardiac changes, importantly elucidating age-related changes. It also is the first to show that MICOS complex may play a role outer membrane mitochondrial structure.
Abstract During aging, muscle gradually undergoes sarcopenia, the loss of function associated with mass, strength, endurance, and oxidative capacity. However, 3D structural alterations mitochondria aging in skeletal cardiac tissues are not well described. Although mitochondrial is decreased capacity, genes responsible for morphological changes during poorly characterized. We measured morphology aged murine gastrocnemius, soleus, using serial block‐face scanning electron microscopy...
Abstract Mitochondria and endoplasmic reticulum (ER) contact sites (MERCs) are protein‐ lipid‐enriched hubs that mediate interorganellar communication by contributing to the dynamic transfer of Ca 2+ , lipid, other metabolites between these organelles. Defective MERCs associated with cellular oxidative stress, neurodegenerative disease, cardiac skeletal muscle pathology via mechanisms poorly understood. We previously demonstrated muscle‐specific knockdown (KD) mitochondrial fusion mediator...
High-resolution 3D images of organelles are paramount importance in cellular biology. Although light microscopy and transmission electron (TEM) have provided the standard for imaging structures, they cannot provide images. However, recent technological advances such as serial block-face scanning (SBF-SEM) focused ion beam (FIB-SEM) tools to create ultrastructural analysis organelles. Here, we describe a standardized protocol using visualization software, Amira, quantify organelle...
While it is commonly thought that microaggressions are isolated incidents, ingrained throughout the academic research institution (Young, Anderson and Stewart 2015; Lee et al. 2020). Persons Excluded from science because of Ethnicity Race (PEERs) frequently experience various academicians, including graduate students, postdocs faculty (Asai 2020; Here, we elaborate on a rationale for concrete actions to cope with diminish acts may otherwise hinder inclusion PEERs. We encourage Science,...
Abstract The success of mentoring derives from active and respectful listening the willingness to learn accept opportunities for personal growth. This shapes every trainee their destined path in science, technology, engineering, mathematics (STEM). act cultivating rapport, asking, pondering meaningful questions, receiving constructive feedback are critical support a productive relationship. Successful STEM can be established allow mentees, especially underrepresented minorities (URMs),...
ABSTRACT Mitochondria and peroxisomes are dynamic signaling organelles that constantly undergo fission, driven by the large GTPase dynamin-related protein 1 (DRP1; encoded DNM1L). Patients with de novo heterozygous missense mutations in DNM1L present encephalopathy due to defective mitochondrial peroxisomal fission (EMPF1) – a devastating neurodevelopmental disease no effective treatment. To interrogate mechanisms which DRP1 cause cellular dysfunction, we used human-derived fibroblasts from...
Abstract Mitochondria are required for energy production and even give brown adipose tissue (BAT) its characteristic color due to their high iron content abundance. The physiological function bioenergetic capacity of mitochondria connected the structure, folding, organization inner‐membrane cristae. During aging process, mitochondrial dysfunction is observed, regulatory balance dynamics often disrupted, leading increased fragmentation in cells. Therefore, it hypothesized that significant...
Maximizing Access to Research Careers (MARC) programs are aimed increase diversity in science, technology, engineering, math, and medicine (STEMM) fields. However, limited eligibility requirements limit the students who may apply similar programs. At Winston-Salem State University, we piloted a series of workshops, collectively termed
Working with multiple mentors is a critical way for students to expand their network, gain opportunities, and better prepare future scholastic or professional ventures. However, from underrepresented groups (UR) are less likely be mentored have access mentors, particularly in science, technology, engineering, mathematics (STEM) fields. We developed implemented workshop, provide the necessary foundation prepared establishing mentorships throughout graduate school. Faculty well-versed area of...
Choosing a mentor requires certain level of introspection for both the and mentee. The dynamics mentorship may change depending on academic status Regardless, mentors should help their trainees grow academically professionally. success an individual in fields science, technology, engineering, mathematics, medicine (STEMM) depends more than intellectual capacity; holistic view encompassing all factors that contribute to scientific achievement is all-important. Specifically, one new method...
Underrepresented faculty have higher burnout rates and lower grant attainment when compared with their non-minority counterparts. Many in science, technology, engineering, mathematics, medicine (STEMM) disciplines, including underrepresented individuals, often difficulty dedicating time to the writing process, trainees being relegated laboratory tasks training years, resulting a lack of practice academic writing. Notably, past studies shown that individuals are than majority Here, we sought...
Identity matters in science, technology, engineering, mathematics, and medicine (STEMM) because it can affect an individual's long-term sense of belonging, which may turn their persistence STEMM. Early K-12 science classes often teach students about the foundational discoveries field, have been predominately made, or at least published, by White men. This homogeneity leave underrepresented individuals STEMM feeling isolated, feel as though they cannot enter fields. study aimed to examine...
Abstract Proximity ligation assays (PLAs) use specific antibodies to detect endogenous protein‐protein interactions. PLAs are a highly useful biochemical technique that allow two proteins within proximity be visualized with fluorescent probes amplified by PCR. While this has gained prominence, the of PLA in mouse skeletal muscle (SkM) is novel. In article, we discuss how method can used SkM study interactions mitochondria‐endoplasmic reticulum contact sites (MERCs). © 2025 The Author(s)....
Abstract OPA1 is a dynamin‐related GTPase that modulates mitochondrial dynamics and cristae integrity. Humans carry eight different isoforms of mice five, all which are expressed as short‐ or long‐form isoforms. These contribute to OPA1's ability control energetics DNA maintenance. However, western blot isolation long short can be difficult. To address this issue, we developed an optimized protocol based on improving running time isolate five in mouse cells tissues. This applied study...