A5101399060- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Probiotics and Fermented Foods
- Cancer Genomics and Diagnostics
- Bacteriophages and microbial interactions
- Metal Extraction and Bioleaching
- Legume Nitrogen Fixing Symbiosis
- Methane Hydrates and Related Phenomena
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
- Oral microbiology and periodontitis research
- Plant Disease Resistance and Genetics
- Wastewater Treatment and Nitrogen Removal
- Microbial Metabolism and Applications
- RNA modifications and cancer
- Actinomycetales infections and treatment
- Bacterial Identification and Susceptibility Testing
- Plant Pathogenic Bacteria Studies
- RNA Research and Splicing
- Microbial Natural Products and Biosynthesis
- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Phagocytosis and Immune Regulation
- Environmental DNA in Biodiversity Studies
University of Auckland
2014-2025
United States Naval Research Laboratory
2019-2024
Emory University
2001-2024
Greenlane Clinical Centre
2024
Siena College
2021-2023
Johns Hopkins University
2023
Taipei Medical University
2022
Virginia Tech
2020-2021
Carilion Clinic
2020-2021
Collaborative Drug Discovery (United States)
2018-2020
We have introduced a targeted mutation in SH2D1A / DSHP SAP , the gene responsible for human genetic disorder X-linked lymphoproliferative disease (XLP). SLAM-associated protein (SAP)-deficient mice had normal lymphocyte development, but on challenge with infectious agents, recapitulated features of XLP. Infection − choriomeningitis virus (LCMV) or Toxoplasma gondii was associated increased T cell activation and IFN-γ production, as well reduction Ig-secreting cells. Anti-CD3-stimulated...
CD47 is a widely expressed cell surface protein that functions as an immune checkpoint in cancer. When by tumor cells, can bind SIRPα on myeloid leading to suppression of phagocytosis and other innate functions. CD47-SIRPα signaling has also been implicated the adaptive antitumor responses, but relevant cellular have yet be elucidated. Therapeutic blockade pathway may stimulate immunity improve cancer therapy. To this end, novel CD47-blocking molecule, ALX148, was generated fusing modified...
Melanins are macromolecules that ubiquitous in nature and impart a large variety of biological functions, including structure, coloration, radiation resistance, free radical scavenging, thermoregulation. Currently, the majority investigations, melanins either chemically synthesized or extracted from animals, which presents significant challenges for large-scale production. Bacteria have been used as biocatalysts to synthesize biomaterials due their fast growth amenability genetic engineering...
Regulation of cytoplasmic dynein and microtubule dynamics is crucial for both mitotic cell division neuronal migration. NDEL1 was identified as a protein interacting with LIS1, the product gene mutated in lissencephaly. To elucidate function vivo, we generated null hypomorphic alleles Ndel1 mice by targeted disruption. Ndel1(-/-) were embryonic lethal at peri-implantation stage like mutants Lis1 heavy chain. In addition, blastocysts failed to grow culture exhibited proliferation defect inner...
SLC25A19 mutations cause Amish lethal microcephaly (MCPHA), which markedly retards brain development and leads to alpha-ketoglutaric aciduria. Previous data suggested that SLC25A19, also called DNC, is a mitochondrial deoxyribonucleotide transporter. We generated knockout mouse model of Slc25a19. These animals had 100% prenatal lethality by embryonic day 12. Affected embryos at 10.5 have neural-tube closure defect with ruffling the neural fold ridges, yolk sac erythropoietic failure,...
COMMD1 (previously known as MURR1) belongs to a novel family of proteins termed the copper metabolism gene MURR1 domain (COMMD) family. The 10 COMMD members are well conserved between vertebrates, but functions most unknown. We recently established that is associated with hepatic overload disorder toxicosis in Bedlington terriers. Recent vitro studies indicate has multiple functions, including sodium transport and NF-kappaB signaling. To elucidate function Commd1 vivo, we generated...
Kytococcus sedentarius (ZoBell and Upham 1944) Stackebrandt et al. 1995 is the type strain of species, phylogenetic interest because its location in Dermacoccaceae, a poorly studied family within actinobacterial suborder Micrococcineae. known for production oligoketide antibiotics as well role an opportunistic pathogen causing valve endocarditis, hemorrhagic pneumonia, pitted keratolysis. It strictly aerobic can only grow when several amino acids are provided medium. The described this...
Odoribacter splanchnicus (Werner et al. 1975) Hardham 2008 is the type species of genus Odoribacter, which belongs to family Porphyromonadaceae in order ‘Bacteroidales’. The interest because members form an isolated cluster within Porphyromonadaceae. This first completed genome sequence a member and fourth from 4,392,288 bp long with its 3,672 protein-coding 74 RNA genes part Genomic Encyclopedia Bacteria Archaea project.
The ability to rapidly assess and optimize heterologous pathway function is critical for effective metabolic engineering. Here, we develop a systematic approach analysis based on correlations between targeted proteins metabolites apply it the microbial production of isopentenol, promising biofuel. Starting with seven-gene pathway, performed correlation reduce complexity identified two as primary determinants efficient isopentenol production. Aided by quantification relevant intermediates,...
Planctomyces brasiliensis Schlesner 1990 belongs to the order Planctomycetales, which differs from other bacterial taxa by several distinctive features such as internal cell compartmentalization, multiplication forming buds directly spherical, ovoid or pear-shaped mother and a wall consisting of proteinaceous layer rather than peptidoglycan layer. The first strains P. brasiliensis, including type strain IFAM 1448T, were isolated water sample Lagoa Vermelha, salt pit near Rio de Janeiro,...
In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated "Enterobacter lignolyticus" SCF1 on minimal media with alkali as the sole source carbon. This organism was anaerobically from tropical forest soils collected Short Cloud Forest site in El Yunque National Puerto Rico, USA, part Luquillo Long-Term Ecological Research Station. At this site, experience strong fluctuations redox potential and are net methane producers. Because its ability grow anaerobically,...
The Wnt/β-catenin signaling pathway plays essential roles in embryonic development and adult tissue homeostasis. Axin is a concentration-limiting factor responsible for the formation of β-catenin destruction complex. Wnt itself promotes degradation Axin. However, underlying molecular mechanism biological relevance this targeting have not been elucidated. Here, we identify SIAH1/2 (SIAH) as E3 ligase mediating Wnt-induced degradation. SIAH proteins promote ubiquitination proteasomal through...
Abstract Background Signal regulatory protein α (SIRPα) is a myeloid-lineage inhibitory receptor that restricts innate immunity through engagement of its cell surface ligand CD47. Blockade the CD47–SIRPα interaction synergizes with tumor-specific antibodies and T-cell checkpoint inhibitors by promoting myeloid-mediated antitumor functions leading to induction adaptive immunity. Inhibition has focused predominantly on targeting CD47, which expressed ubiquitously contributes accelerated blood...
Mutant src(-/-) mice have osteopetrosis resulting from defective osteoclasts, the cells that resorb bone. However, signaling pathways involving Src family members in osteoclasts remain unclear. We demonstrate expression of a truncated molecule, Src251, lacking kinase domain, induces wild-type and src(+/-) worsens by novel mechanism, increased osteoclast apoptosis. Induction apoptosis Src251 requires functional SH2, but not an SH3, domain is associated with reduced AKT activity. Expression...
Haliangium ochraceum Fudou et al. 2002 is the type species of genus in myxococcal family 'Haliangiaceae'. Members are first halophilic myxobacterial taxa described. The cells follow a multicellular lifestyle highly organized biofilms, called swarms, they decompose bacterial and yeast as most myxobacteria do. fruiting bodies contain particularly small coccoid myxospores. H. encodes actin homologue identified genome. Here we describe features this organism, together with complete genome...
ABSTRACT Desulfosporosinus species are sulfate-reducing bacteria belonging to the Firmicutes . Their genomes will give insights into genetic repertoire and evolution of sulfate reducers typically thriving in terrestrial environments able degrade toluene ( youngiae ), reduce Fe(III) meridiei , orientis grow under acidic conditions acidiphilus ).
Spirochaeta coccoides Dröge et al. 2006 is a member of the genus Ehrenberg 1835, one oldest named genera within Bacteria. S. an obligately anaerobic, Gram-negative, non-motile, spherical bacterium that was isolated from hindgut contents termite Neotermes castaneus. The species interest because it may play important role in digestion breakdown products cellulose and hemicellulose gut. Here we provide taxonomic re-evaluation for strain SPN1T, based on physiological genomic characteristics,...