A5101895570- Sirtuins and Resveratrol in Medicine
- Autophagy in Disease and Therapy
- Enzyme Production and Characterization
- Phytase and its Applications
- Protein Kinase Regulation and GTPase Signaling
- Ovarian cancer diagnosis and treatment
- Enzyme Structure and Function
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Breast Cancer Treatment Studies
- Adipose Tissue and Metabolism
- Endometrial and Cervical Cancer Treatments
- HER2/EGFR in Cancer Research
- Signaling Pathways in Disease
- Cellular transport and secretion
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- Cancer Treatment and Pharmacology
- Cancer-related gene regulation
- Cassava research and cyanide
- Pancreatic function and diabetes
- Genomics and Chromatin Dynamics
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- PARP inhibition in cancer therapy
Kunming Medical University
2012-2025
Lanzhou University
2018-2025
Peking University
2025
Peking University Cancer Hospital
2025
Goldwind (China)
2024
Lanzhou University Second Hospital
2023
Universidad del Noreste
2023
Asan Medical Center
2022
Southern University of Science and Technology
2022
Jinan University
2022
Chronic inflammation is a major contributing factor in the pathogenesis of many age-associated diseases. One central protein that regulates NF-κB, activity which modulated by post-translational modifications as well association with co-activator and co-repressor proteins. SIRT1, an NAD(+)-dependent deacetylase, has been shown to suppress NF-κB signaling through deacetylation p65 subunit resulting reduction inflammatory responses mediated this transcription factor. The role SIRT1 regulation...
Metformin is currently a strong candidate anti-tumor agent in multiple cancers. However, its effectiveness varies among different cancers or subpopulations, potentially due to tumor heterogeneity. It thus remains unclear which hepatocellular carcinoma (HCC) patient subpopulation(s) can benefit from metformin treatment. Here, through genome-wide CRISPR-Cas9-based knockout screen, we find that DOCK1 levels determine the effects of and synthetic lethal target HCC. Mechanistically, promotes...
Summary This past decade has seen the identification of numerous conserved genes that extend lifespan in diverse species, yet number compounds is relatively small. A class called STACs, which were identified as activators Sir2/SIRT1 NAD + ‐dependent deacetylases, lifespans multiple species a Sir2‐dependent manner and can delay onset age‐related diseases such cancer, diabetes neurodegeneration model organisms. Plant‐derived STACs fisetin resveratrol have several liabilities, including poor...
Epalrestat is a noncompetitive and reversible aldose reductase inhibitor used for the treatment of diabetic neuropathy. This study assumed that epalrestat had protective effect on peripheral nerve injury by suppressing expression in nerves diabetes mellitus rats. The high-fat high-carbohydrate model rats were established intraperitoneal injection streptozotocin. Peripheral neuropathy occurred these after sustaining high blood glucose 8 weeks. At 12 weeks streptozotocin injection,...
The tumor suppressor p53 is an essential transcription factor that sensitively regulates cellular responses to various stresses. Acetylation, a critically important posttranslational modification of p53, induced in response P53 acetylation level strongly correlates with protein stability and activity. steady-state balanced by dynamic deacetylation. Despite the function being well studied, how steady state regulated stresses remains unclear. In particular, regulation deacetylase activities...
Three unreported ent-abietane-type norditerpene lactones, euphohelides A–C (1–3), and 11 known analogs (4–14) were isolated from the whole plants of Euphorbia helioscopia L. Euphohelide A (1) is an unprecedented 2-nor-ent-abietane lactone bearing a unique 5/6/6/5 tetracyclic system. Euphohelides B (2) C (3) possess 2-nor-6/6/6/5 2,3-dinor-5/6/6/5 dilactone moieties, respectively. Their structures established by spectroscopic methods, computational ECD, X-ray crystallographic analyses....
A gene putatively identified as the Archaeoglobus fulgidus inositol-1-phosphate synthase (IPS) was overexpressed to high level (about 30-40% of total soluble cellular proteins) in Escherichia coli. The recombinant protein purified homogeneity by heat treatment followed two column chromatographic steps. native enzyme a tetramer 168 +/- 4 kDa (subunit molecular mass 44 kDa). At 90 degrees C K(m) values for glucose-6-phosphate and NAD(+) were estimated 0.12 0.04 mM 5.1 0.9 microM, respectively....
The aim of this study was to investigate pathophysiological alterations and oxidative stress in various stages streptozotocin (STZ)‑induced diabetes mellitus (DM) rats. Male Sprague-Dawley rats (120) were randomized into DM control groups. Body mass, plasma glucose, glycated hemoglobin (HbA1c), superoxide dismutase (SOD), catalase (CAT) glutathione peroxidase (GPx) levels, as well aldose reductase (AR) activities, brain tissue serum determined. Electron microscopy used observe neuron vessel...
Cervical carcinoma is the third most common cause of cancer in women with a significant challenge clinical treatment. Human papillomavirus (HPV) strongly responsible for cervical carcinoma. Here, we show increased expression level heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) HPV-associated cells including HeLa, Caski, and SiHa cells, especially HeLa cells. We provide evidence that HNRNPA1 closely related to cell proliferation, invasion, migration. Emerging histone modifications...
Five complex indoline alkaloids, alstolarsines A-E (1-5) possessing two unprecedented carbon skeletons of 6/5/5/7/6/6(5) fused polycyclic systems, were isolated from Alstonia scholaris. Their structures characterized using various methods including spectroscopic data, ECD spectra, and single-crystal X-ray diffraction. The inhibitory activities A-D (1-4) on DRAK2 phosphorylation ATP-citrate lyase evaluated, but all them inactive.
Existing treatments for cervical cancer have side effects on the human body. Some lactobacilli inhibit tumour progression in a strain-specific manner without toxic effects. We explored whether Lactobacillus brevis YNH isolated from vagina has anti-cervical by performing Cell Counting Kit-8 assays, flow cytometry, JC-1 staining, and western blotting. Transcriptome sequencing was performed to determine possible mechanism. Xenograft model mice that were orally administered used validate...
Aqueous zinc-ion batteries have attracted great attention due to their advantages in energy storage. However, the existence of dendrites and a series side reactions during charge discharge...
Preclinical data have shown the potential of intraductal administration chemotherapy for breast cancer prevention. Direct translation this work has been stymied by anatomical differences between rodents (one duct per teat) and women (5-9 ductal systems breast). The objective phase I study was to show safety feasibility drugs into multiple ducts within one in awaiting mastectomy treatment invasive cancer. Thirty subjects were enrolled dose escalation conducted at a single center Beijing,...
Inositol monophosphatase (EC 3.1.3.25) in hyperthermophilic archaea is thought to play a role the biosynthesis of di-myo-inositol-1,1'-phosphate (DIP), an osmolyte unique hyperthermophiles. The Methanococcus jannaschii MJ109 gene product, sequence which substantially homologous that human inositol monophosphatase, exhibits activity but with substrate specificity broader than those bacterial and eukaryotic monophosphatases (it can also act as fructose bisphosphatase). To understand its well...
Several hyperthermophilic organisms contain an unusual phosphatase that has dual activity toward inositol monophosphates and fructose 1,6-bisphosphate. The structure of the second member this family, FBPase/IMPase from Archaeoglobus fulgidus (AF2372), been solved. This enzyme shares many kinetic structural similarities with a previously solved Methanococcus jannaschii (MJ0109). It also shows some differences in divalent metal ion binding as well variations at dimer interface correlate...
A kinetic comparison of the hydrolase and transferase activities two bacterial phospholipase D (PLD) enzymes with little sequence homology provides insights into mechanistic differences also more general role Ca(2+) in modulating PLD reactions. Although PLDs exhibit similar substrate specificity (phosphatidylcholine preferred), sensitivity to aggregation or Ca(2+), pH optima are quite distinct. Streptomyces sp. PMF PLD, a member superfamily, generates both products parallel, consistent...
Phospholipase D (PLD), an important enzyme involved in signal transduction mammals, is also secreted by many microorganisms. A highly conserved HKD motif has been identified most PLD homologs the superfamily. However, Ca(2+)-dependent from Streptomyces chromofuscus exhibits little homology to other PLDs. We have cloned (using DNA isolated ATCC type strain), overexpressed Escherichia coli (two expression systems, pET-23a(+) and pTYB11), purified S. PLD. Based on attempts at sequence alignment...
Natural products frequently have unique physiological activities and new action mechanisms due to their structural diversity novelty, are an important source for innovative drugs lead compounds. We present herein that natural product santamarine targeted thioredoxin reductase (TrxR) weaken its antioxidative function in cells, accompanied by accumulation of high levels reactive oxygen species (ROS), finally induced a mechanism tumor cell oxidative stress-mediated apoptosis. TrxR knockdown or...
WD repeat and SOCS box containing protein (WSB) molecules have important roles in tumorigenesis. WSB1 is dysfunctional many malignancies. However, the effects of WSB2 tumors, including melanoma, not been reported. Here, we investigated melanoma cell proliferation, cycle progression, migration, underlying mechanisms.First, expression levels their association with clinicopathological features were evaluated human tissue samples. Then, was knocked down, using specific shRNA, A375 G361 cells....