A5072712112- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Antimicrobial Resistance in Staphylococcus
- Escherichia coli research studies
- Urinary Tract Infections Management
- Microbial Natural Products and Biosynthesis
- Gut microbiota and health
- Pharmaceutical and Antibiotic Environmental Impacts
- Synthesis and Biological Evaluation
- Educator Training and Historical Pedagogy
- Advanced Fluorescence Microscopy Techniques
- Essential Oils and Antimicrobial Activity
- Insect and Pesticide Research
- Cancer therapeutics and mechanisms
- Click Chemistry and Applications
- Cell Image Analysis Techniques
- Graphene and Nanomaterials Applications
- Bee Products Chemical Analysis
- 3D Printing in Biomedical Research
- Global Education and Multiculturalism
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Nanoparticles: synthesis and applications
University of Technology Sydney
2016-2025
University of Wollongong
2024
University of Sheffield
2011-2019
Sanford Broadway Medical Center
2017
University of Cincinnati
2016
Summary In Bacillus subtilis , EzrA is involved in preventing aberrant formation of FtsZ rings and has also been implicated the localization cycle Pbp1. We have identified orthologue Staphylococcus aureus to be essential for growth cell division this organism. Phenotypic analyses following titration levels S. shown that protein required peptidoglycan synthesis as well assembly divisome at midcell cytokinesis. Protein interaction studies revealed forms a complex with both cytoplasmic...
Lipoteichoic acid (LTA) is an important cell wall component of Gram-positive bacteria. In Staphylococcus aureus it consists a polyglycerolphosphate-chain that retained within the membrane via glycolipid. Using immunofluorescence approach, we show here LTA polymer not surface exposed in S. aureus, as can only be detected after digestion peptidoglycan layer. mutants lacking are enlarged and aberrant positioning septa, suggesting link between synthesis division process. bacterial two-hybrid...
The threat of antimicrobial resistance to human health has prompted interest in complex, natural products with activity. Honey been an effective topical wound treatment throughout history, predominantly due its broad-spectrum Unlike traditional antibiotics, honey-resistant bacteria have not reported; however, honey remains underutilized the clinic part a lack understanding mechanism action. Here, we demonstrate that affects multiple processes bacteria, and this is explained by major...
Wide-field fluorescence microscopy at high magnification was used to study the intracellular binding site of Rubb16 in Escherichia coli. Upon incubation E. coli cells minimum inhibitory concentration, localised ribosomes with no significant DNA observed. Furthermore, condensed when they existed as polysomes. It is postulated that condensation polysomes would halt protein production, and thereby inhibit bacterial growth. The results this indicate family inert dinuclear ruthenium complexes...
Productive bacterial cell division and survival of progeny requires tight coordination between chromosome segregation to ensure equal partitioning DNA. Unlike rod-shaped bacteria that undergo in one plane, the coccoid human pathogen Staphylococcus aureus divides three successive orthogonal planes, which a different spatial control compared cells. To gain better understanding how this is regulated S. aureus, we investigated proteins associate with FtsZ divisome. We found DnaK, well-known...
A priority pathogen is capable to evolve stable resistance characteristics that still manifest after discontinuation of the nanosilver exposure.
Methicillin-resistant Staphylococcus aureus (MRSA) has proven to be an imminent threat public health, intensifying the need for novel therapeutics. Previous evidence suggests that cannabinoids harbour potent antibacterial activity. In this study, a group of previously inaccessible phytocannabinoids and synthetic analogues were examined potential The minimum inhibitory concentrations dynamics bacterial inhibition, determined through resazurin reduction time-kill assays, revealed activity...
Summary Bacterial cell division is a fundamental process that requires the coordinated actions of number proteins which form complex macromolecular machine known as divisome. The membrane‐spanning DivIB and its orthologue FtsQ are crucial divisome components in G ram‐positive ram‐negative bacteria respectively. However, role almost all integral proteins, including , still remains largely unknown. Here we show extracellular domain able to bind peptidoglycan have mapped binding β subdomain....
Inhibition of cell division is critical for viability under DNA-damaging conditions. DNA damage induces the SOS response that in bacteria inhibits while repairs are being made. In coccoids, such as human pathogen, Staphylococcus aureus, this process remains poorly studied. Here, we identify SosA staphylococcal SOS-induced inhibitor. Overproduction division, sosA inactivation sensitizes cells to genotoxic stress. a small, predicted membrane protein with an extracellular C-terminal domain...
Abstract The killing mechanism of many antibiotics involves the induction DNA damage, either directly or indirectly, which activates SOS response. RecA, master regulator response, has shown to play a central role in evolution resistance fluoroquinolones, even after short-term exposure. While this paradigm is well established for DNA-damaging antibiotics, it remains unclear whether β-lactams elicit similar dynamics depend on RecA and SOS-mediated mechanisms. In study, we observed rapid stable...
Uropathogenic Escherichia coli (UPEC) can undergo extensive filamentation in the host during acute urinary tract infections (UTIs). It has been hypothesised that this morphological plasticity allows bacteria to avoid immune responses such as macrophage engulfment. However, it is still unclear what properties of filaments are important macrophage-bacteria interactions. The aim work was investigate contribution bacterial biophysical parameters, cell size and shape, physiological surface...
Uropathogenic Escherichia coli (UPEC) are a major cause of urinary tract infection (UTI), one the most common infectious diseases in humans. UPEC increasingly associated with resistance to multiple antibiotics. This includes third-generation cephalosporins, class antibiotics frequently used treat UTI.We employed high-throughput genome-wide screen using saturated transposon mutagenesis and directed insertion-site sequencing (TraDIS) together phenotypic assessment identify key genes required...
Rod-shaped bacteria such as Escherichia coli can regulate cell division in response to stress, leading filamentation, a process where growth and DNA replication continues the absence of division, resulting elongated cells. The classic example stress is damage which results activation SOS response. While inhibition during has traditionally been attributed SulA E. coli, previous report suggests that e14 prophage may also encode an SOS-inducible inhibitor, previously named SfiC. However, exact...
Neurological diseases are among the leading causes of disability and death worldwide remain difficult to treat. Tissue engineering offers avenues test potential treatments; however, development biologically accurate models brain tissues remains challenging. Given their neurogenic availability, adipose-derived stem cells (ADSCs) interest for creating neural models. While progress has been made in differentiating ADSCs into cells, differentiation 3D environments, which more representative vivo...
The killing mechanism of many antibiotics involves the induction DNA damage, either directly or indirectly, which triggers SOS response. RecA, master regulator response, plays a crucial role in driving evolution resistance to fluoroquinolone treated with single dose ciprofloxacin. However, precise roles RecA and responses development under short-term β-lactam exposure remain unclear. In present study, we observed fast (20-fold increase MIC 8 hours) E. coli after deleting exposing bacteria...
The killing mechanism of many antibiotics involves the induction DNA damage, either directly or indirectly, which triggers SOS response. RecA, master regulator response, plays a crucial role in driving evolution resistance to fluoroquinolone treated with single dose ciprofloxacin. However, precise roles RecA and responses development under short-term β-lactam exposure remain unclear. In present study, we observed fast (20-fold increase MIC 8 hours) E. coli after deleting exposing bacteria...
The bacterial cell division protein FtsZ is conserved in most bacteria and essential for viability. There have been concerted efforts developing inhibitors that target as potential antibiotics. Key to this an in-depth understanding of structure at the molecular level across diverse species ensure high affinity a variety clinically relevant pathogens. In study, we show structures differ three ways: (1) H7 helix curvature; (2) dimensions interdomain cleft; (3) opening/closing mechanism cleft,...