A5088699817- Neurobiology and Insect Physiology Research
- Developmental Biology and Gene Regulation
- Plant Molecular Biology Research
- Invertebrate Immune Response Mechanisms
- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Hippo pathway signaling and YAP/TAZ
- Genetics, Aging, and Longevity in Model Organisms
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Optical Coherence Tomography Applications
- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- Protist diversity and phylogeny
- Neurogenesis and neuroplasticity mechanisms
- Wnt/β-catenin signaling in development and cancer
- Parkinson's Disease Mechanisms and Treatments
- RNA Research and Splicing
- Signaling Pathways in Disease
- Neurotransmitter Receptor Influence on Behavior
- Infrared Thermography in Medicine
- Photoacoustic and Ultrasonic Imaging
- Thermoregulation and physiological responses
- Cannabis and Cannabinoid Research
- 14-3-3 protein interactions
Temasek Life Sciences Laboratory
2014-2024
National University of Singapore
2015-2024
Korea National Institute of Health
2024
Beijing Forestry University
2024
Chang Gung University
2017-2021
Pusan National University
2021
Duke-NUS Medical School
2009-2020
Singapore Centre for Environmental Life Sciences Engineering
2011-2020
National Cancer Center
2018
Industrial Technology Research Institute
2016
ABSTRACT Apoptosis and autophagy are morphologically distinct forms of programmed cell death. While occurs during the development diverse organisms has been implicated in tumorigenesis, little is known about molecular mechanisms that regulate this type Here we show steroid-activated death Drosophila salivary glands by autophagy. Expression p35 prevents DNA fragmentation partially inhibits changes cytosol plasma membranes dying glands, suggesting caspases involved The steroid-regulated BR-C,...
The choice of self-renewal versus differentiation is a fundamental issue in stem cell and cancer biology. Neural progenitors the Drosophila post-embryonic brain, larval neuroblasts (NBs), divide asymmetrically cell-like fashion to generate self-renewing NB Ganglion Mother Cell (GMC), which divides terminally produce two differentiating neuronal/glial daughters. Here we show that Aurora-A (AurA) acts as tumor suppressor by suppressing promoting neuronal differentiation. In aurA...
Mutations in the leucine-rich repeat kinase 2 ( LRRK2 ) gene are currently recognized as most common genetic cause of parkinsonism. Among large number mutations identified to date, G2019S variant is common. In Asia, however, another variant, G2385R, appears occur more frequently. To better understand contribution different variants toward disease pathogenesis, we generated transgenic Drosophila over-expressing various human alleles, including wild type, G2019S, Y1699C, and G2385R LRRK2. We...
Mutations in parkin and LRRK2 together account for the majority of familial Parkinson's disease (PD) cases. Interestingly, recent evidence implicates involvement mitochondrial homeostasis. Supporting this, we show here by means Drosophila model system that, like parkin, mutations induce pathology flies when expressed their flight muscles, toxic effects which can be rescued coexpression. When specifically fly dopaminergic neurons, mutant results appearance significantly enlarged mitochondria,...
Mutations in the parkin gene are a predominant cause of familial parkinsonism. Although initially described as recessive disorder, emerging evidence suggest that single mutations alone may confer increased susceptibility to Parkinson's disease. To better understand effects vivo , we generated transgenic Drosophila overexpressing two human missense mutants, R275W and G328E. Transgenic flies overexpress R275W, but not wild-type or G328E, display an age-dependent degeneration specific...
Pruning that selectively eliminates unnecessary axons/dendrites is crucial for sculpting the nervous system during development. During Drosophila metamorphosis, dendrite arborization neurons, ddaCs, prune their larval dendrites in response to steroid hormone ecdysone, whereas mushroom body γ neurons specifically eliminate axon branches within dorsal and medial lobes. However, it unknown which E3 ligase directs these two modes of pruning. Here, we identified a conserved SCF ubiquitin plays...
Despite expressing stem cell self-renewal factors, intermediate progenitor cells possess restricted developmental potential, which allows them to give rise exclusively differentiated progeny rather than progeny. Failure restrict the potential can allow revert into aberrant that might contribute tumorigenesis. Insight stable restriction of in could improve our understanding development and growth tumors, but mechanisms involved remain largely unknown. Intermediate neural progenitors (INPs),...
Significance Final body size determination in animals remains a long-standing puzzle the field of developmental biology. In insects, insulin/insulin-like growth factor signaling (IIS) and steroid hormone 20-hydroxyecdysone (20E) are mainly responsible for rate period, respectively, consequently final size. Here, we report that 20E activates AMPK by up-regulating its gene expression inducing sugar starvation. turn, protein phosphatase 2A, which further dephosphorylates inactivates key...
The asymmetric division of Drosophila neuroblasts involves the basal localization cell fate determinants and generation an asymmetric, apicobasally oriented mitotic spindle that leads to formation two daughter cells unequal size. These features are thought be controlled by apically localized protein complex comprising signaling pathways: Bazooka/Drosophila atypical PKC/Inscuteable/DmPar6 Partner inscuteable (Pins)/Gαi; in addition, Gβ13F is also required. However, role Gαi hierarchical...
The control of self-renewal and differentiation neural stem progenitor cells is a crucial issue in cell cancer biology. Drosophila type II neuroblast lineages are prone to developing impaired homeostasis if the limited self-renewing potential intermediate progenitors (INPs) unrestrained. Here, we demonstrate that SWI/SNF chromatin remodeling Brahma (Brm) complex functions cooperatively with another factor, Histone deacetylase 3 (HDAC3) suppress formation ectopic neuroblasts. We show multiple...
Heterotrimeric G proteins mediate asymmetric division of Drosophila neuroblasts. Free Gβγ appears to be crucial for the generation an mitotic spindle and consequently daughter cells distinct size. However, how is released from inactive heterotrimer remains unclear. Here we show that Locomotion defects (Loco) interacts colocalizes with Gαi and, through its GoLoco motif, acts as a guanine nucleotide dissociation inhibitor (GDI) Gαi. Simultaneous removal two motif proteins, Loco Pins, results...
Drosophila larval brain neural stem cells, also known as neuroblasts, divide asymmetrically to generate a self-renewing neuroblast and ganglion mother cell (GMC) that divides terminally produce two differentiated neurons or glia. Failure of asymmetric division can result in hyperproliferation phenotype resembling tumors. Here we have identified Protein phosphatase 2A (PP2A) tumor-suppressor inhibit self-renewal neuroblasts. Supernumerary neuroblasts are generated at the expense PP2A mutants....