A5042904295- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Xenotransplantation and immune response
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Organ Transplantation Techniques and Outcomes
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Liver physiology and pathology
- Innovation and Socioeconomic Development
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Inhalation and Respiratory Drug Delivery
- Bone Tissue Engineering Materials
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Extracellular vesicles in disease
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pancreatic function and diabetes
- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
Sichuan University
2018-2025
Ministry of Agriculture
2025
Fujian Agriculture and Forestry University
2025
West China Hospital of Sichuan University
2018-2024
Idiopathic pulmonary fibrosis (IPF) is characterized by persistent tissue injury, dysregulated wound healing, and extracellular matrix (ECM) deposition myofibroblasts (MFs) through the fibroblast-to-myofibroblast transition (FMT). Implicit in FMT process are changes ECM cellular topology, but their relationship with lung fibroblast phenotype has not been explored. We engineered topological mimetics of alignment cues (anisotropy/isotropy) using decellularized micropattern arrays investigated...
This study aims to evaluate the effectiveness and safety of spheroid reservoir bioartificial liver (SRBAL) with porcine hepatocyte organoids in a preclinical nonhuman primate model acute failure (ALF).Methods: Thirty healthy rhesus monkeys were infused α-amanitin lipopolysaccharide randomized into five groups (ALF alone control group; sham no-cell SRBAL treatment A, B C started at 12 h, 24 h 36 after induction ALF, respectively).Animals continuously treated device for 6 followed up 336...
The natural liver extracellular matrix (ECM) achieved by decellularization holds great potential in the fields of tissue engineering and regenerative medicine. Additionally, use crosslinking agents on ECM to stabilize its ultrastructure enhance scaffold durability is gaining interest engineering. objective this study was compare properties porcine crosslinked with different (glutaraldehyde, genipin, quercetin) find best strategy for producing a decellularized optimal stable characteristics...
Three-dimensional (3D) culture via micropattern arrays to generate cellular spheroids seems a promising in vitro biomimetic system for liver tissue engineering applications, such as drug screening. Recently, organ-derived decellularized extracellular matrix emerges arguably the most bioink. Herein, (DLM)-derived array chips were developed fabricate size-controllable and arrangement-orderly HepG2 The porcine DLM was obtained by removal of components then ground into powder, followed...
This study aims to evaluate ECM-coated micropattern arrays derived from decellularization of native porcine lungs as a novel three-dimensional cell culture platform.ECM was exploited prepare hydrogels. Then, dECM-coated were fabricated at four different diameters (50, 100, 150 and 200 μm) using polydimethylsiloxane (PDMS). Two lung cancer lines, A549 H1299, tested on array platform for adhesion, distribution, proliferation, viability, phenotype expression, drug screening the cytotoxicity...
Introduction: Gene-edited pigs have become prominent models for studying human disease mechanisms, gene therapy, and xenotransplantation. CRISPR (clustered regularly interspaced short palindromic repeats)/CRISPR-associated 9 (CRISPR/Cas9) technology is a widely employed tool generating gene-edited pigs. Nevertheless, delivering CRISPR/Cas9 to pre-implantation embryos has traditionally posed challenges due its reliance on intricate micromanipulation equipment specialized techniques, resulting...
Retrograde signaling regulates plant senescence, but the role of vacuoles in this process remains unclear. Here, we demonstrate that rice vacuolar H+-ATPase subunit A (OsVHA-A) localizes to both cytoplasm and nucleus. Sucrose treatment increased OsVHA-A expression nuclear accumulation, while darkness reduced it. Methyl jasmonate (MeJA) initially promoted translocation decreased it upon prolonged exposure. Downregulation accelerated MeJA-induced leaf senescence delayed darkness-induced...
Abstract Background. Recent studies have shown promising results using decellularized extracellular matrix (dECM) matrikines-based hydrogel as attractive strategies for preventing and alleviating fibrosis. Methods & Results. Porcine lung decellularization pepsin digestion were used to prepare the dECM hydrogel. Proteomic analysis revealed that was enriched in glycoproteins, collagens, laminins, fibrinogen, held receptors, bound growth factors. With porous structures good mechanical...
One of the key steps using CRISPR/Cas9 to obtain gene-edited cells used in generating animals combined with somatic cell nuclear transplantation (SCNT) is harvest monoclonal genetic modifications. However, primary as donors always grow slowly and fragile after a series gene-editing operations. The extracellular matrix (ECM) formulated directly from different organs comprises complex proteins growth factors that can improve regulate cellular functions cells. Herein, sodium lauryl ether...
CRISPR/Cas9 technology, combined with somatic cell nuclear transplantation (SCNT), represents the primary approach to generating gene-edited pigs. The inefficiency in acquiring donors is attributed low editing and delivery efficiency, both closely linked selection of forms. However, there currently no direct method evaluate efficiency porcine genomes. A platform based on fluorescence reporting signals micropattern arrays was developed this study, visually assess gene editing. optimal...
Hepatocyte transplantation and bioartificial liver (BAL) systems hold significant promise as less invasive alternatives to traditional transplantation, providing crucial temporary support for patients with acute chronic failure. Although human hepatocytes are ideal, their use is limited by ethical concerns donor availability, leading the of porcine in BAL due functional similarities. Recent advancements gene-editing technology have improved organ xenotransplantation clinical trials...
The challenge of using bioengineered liver lies in sustaining the quantity high-quality hepatocytes and vasculature for blood perfusion. We characterized heparinization a porcine decellularized scaffold (DLS) as carrier to support hepatocyte angiogenesis, thereby developing functional vascularized hepatic tissue useful treat injury.The DLS was obtained by removal cellular components then subjected end-point attachment technique. heparinized DLSs were recellularized with rat spheroids...
The shortage of human hepatocytes continues to be a significant limitation for the widespread application hepatocyte transplantation and bioartificial liver (BAL) support therapy. Recombinant activation gene 2 (Rag2) fumarylacetoacetate hydrolase (Fah)-deficient mice could highly repopulated with hepatocytes. However, Fah/Rag2-deficient can only produce up 1 × 108 per mouse. We hypothesized that 2-10 1010 produced pig, which is an adequate supply BAL In novel approach, we used stably...
In the world's first pig-to-human cardiac xenotransplant, elevated levels of porcine cytomeg-alovirus (PCMV) are considered key factors contributing to patient mortality. This has renewed attention PCMV, a virus widely prevalent in pigs. Currently, there no effective drugs or vaccines targeting and its high detection difficulty poses challenges for prevention control research. this study, antiviral small hairpin RNA (shRNA) was selected inserted into Rosa26 miR-17-92 loci pigs via...
In the world’s first pig-to-human cardiac cytomegalovirus (PCMV), xenotransplant and elevated levels of porcine key factors contributing to patient mortality were considered. This has renewed attention on PCMV, a virus widely prevalent in pigs. Currently, there are no effective drugs or vaccines targeting its high detection difficulty poses challenges for prevention control research. this study, antiviral small hairpin RNA (shRNA) was selected inserted into Rosa26 miR-17-92 loci pigs via...
CRISPR/Cas9 technology in conjunction with somatic cell nuclear transplantation (SCNT) provides the primary approach to producing gene-edited pigs, and targeting donors is crucial. Gene-edited are inefficient due poor editing efficiency low delivery efficiency, which highly associated form selection. Nevertheless, there not a straightforward method evaluate on porcine genome. In this study, fluorescence report signal micropattern arrays-based platform was developed visually assess of...
Various liver diseases result in failure, and transplantation artificial support systems are emerging as alternative therapies for these disease. However, both treatments depending on cell resources, so isolating sufficient number of functional primary hepatocytes is a most important early issue. Isolated also suitable system the physiological, pharmacological toxicological study hepatic uptake, metabolism, excretion toxicity. In this paper, methods hepatocyte isolation reviewed; culture...