A5025108724- Bacteriophages and microbial interactions
- Burkholderia infections and melioidosis
- Escherichia coli research studies
- Brucella: diagnosis, epidemiology, treatment
- Monoclonal and Polyclonal Antibodies Research
- Subcritical and Supercritical Water Processes
- Transgenic Plants and Applications
- Pharmacological Effects of Natural Compounds
- Microbial Inactivation Methods
- Viral gastroenteritis research and epidemiology
- Antimicrobial Resistance in Staphylococcus
- Legionella and Acanthamoeba research
- Plant-based Medicinal Research
- Biochemical and Structural Characterization
- Bacterial Genetics and Biotechnology
- Bacillus and Francisella bacterial research
- Yersinia bacterium, plague, ectoparasites research
Objective of study is to prepare hybridomas-producers monoclonal antibodies brucellosis agent antigens. Materials and methods. B. abortus, melitensis, suis strains from the State collection microorganisms 48th Central Research Institute Affiliated Branch BALB/c mice. Hybridization was performed as described by G.Kohler C.Milstein in modification Fazekas De St. Scheidegger D. The specific activity immune sera, hybridoma supernatants, ascites fluid, antibody preparations using ELISA. Results...
Objective of the study was development immune-enzymatic monoclonal test-kit for detecting glanders and melioidosis agents. Materials methods. We used microbial cultures hybrid cell lines obtained from collection «48 th Central Research Institute» Ministry Defense Russian Federation. Hybridoma cells were incubated in peritoneal cavity BALB/c mice. Preparations antibodies isolated ascetic fluids through precipitation with ammonium sulfate purification by means ion-exchange chromatography....
Objective of the study was development experimental ELISA tests and lateral flow immunoassays for detection staphylococcal enterotoxins, A B types. Materials methods . Hybridomas, producing monoclonal antibodies against enterotoxins from State Collection Affiliated Branch “48th Central Research Institute” Ministry Defense Russian Federation, BALB/c mice were used in research. Hybridoma cells incubated culture flasks peritoneal cavity mice. Monoclonal isolated ascitic fluids through...
Aim. Obtaining hybridomas, stable producing specific monoclonal antibodies against Burkholderia mallei and pseudomallei antigens. Materials methods. The microbial cultures from State Collection of Microorganisms the Branch 48 CSRI Defense Ministry Russian Federation (Kirov) BALB/c mouse were used in research. Hybridization B lymphocytes with SP2/0-Ag14 myeloma cells was performed by G.Kohler C.Milstein procedure De St. Fazekas D.Scheidegger modification. activity immune sera, hybridoma...
Objective of the study was to develop monoclonal immunoassay for detection pseudotuberculosis agent, serogroup I. Materials and methods. Specific components, that were used immune-chromatographic test-system development mouse antibodies hybrid cell lines, obtained lipopolysaccharide antigen outer membrane pathogen's «cold» variant (YP-101N2V4, YP-105S5A10); rabbit anti-species against murine immunoglobulins. Particles, (30±2) nm in diameter, prepare colloidal gold-antibody conjugate....
Carried out have been two experimental studies on hybridization of mouse myeloma cells and lymphocytes BALB/c mice immunized with inactivated microbe Francisella tularensis cultures. As a result obtained hybridomas-producers monoclonal antibodies (MAb) specific to the antigens tularemia agent. Evaluated prospects its application for detection agent under discussion using enzyme-linked immunoassay. Established is fact that produced by 31G1F10, 32E5D3, 35B11C8, 36C2F11 hybridomas make it...
Developed is the highly sensitive and specific immuno-enzyme test-system, which perspective for detection of L. pneumophilia , serogroup 1. Isolated are three hybrid cell lines that secrete monoclonal antibodies to epitopes 1 lipopolysaccharide antigen. Hyper immune rabbit sera, characterized by activity specificity, obtained using
Introduction. The aim of the work was development enzyme immunoassay for detecting I and II types shiga-like toxins assessment it diagnostic properties. Materials methods. For research, we used hybridomas producing monoclonal antibodies to II, obtained at branch Federal State Budgetary Institution “48 Central Research Institute” Ministry Defense Russian Federation (Kirov); BALB/c mice; II. Hybridoma cells were cultured in culture flasks peritoneal cavity mice. Monoclonal isolated from...
Objective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I II types shiga-like toxins. Materials methods. Shiga-like toxins obtained in “48thCentral Research Institute” Ministry Defense Russian Federation (Kirov), BALB/c mice, myeloma cells SP2/0-Ag14 were used research. Immune splenocytes fused according to G. Kohler C. Milstein method De St. Fazekas D. Scheidegger modifcation using 50 % polyethylene glycol. Hybrid specifc cloned by limited...