A5065980324- Burkholderia infections and melioidosis
- Bacteriophages and microbial interactions
- Transgenic Plants and Applications
- Monoclonal and Polyclonal Antibodies Research
- Bacillus and Francisella bacterial research
- Pharmacological Effects of Natural Compounds
- Escherichia coli research studies
- Brucella: diagnosis, epidemiology, treatment
- Subcritical and Supercritical Water Processes
- Biochemical and Structural Characterization
- Bacterial Genetics and Biotechnology
- SARS-CoV-2 and COVID-19 Research
- Plant-based Medicinal Research
- Antimicrobial Resistance in Staphylococcus
- Microbial Inactivation Methods
- Insect and Pesticide Research
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Yersinia bacterium, plague, ectoparasites research
Ministry of Defence of the Russian Federation
2022
The aim of the work was to obtain and characterize hybridomas producing monoclonal antibodies antigens coronavirus SARS‑CoV‑2, promising for construction diagnostic immunochemical tests. Materials methods. Recombinant nucleocapsid receptor binding fragment spike protein SARS‑CoV‑2 were used immunization BALB/c mice. Antigens absorbed on aluminium hydroxide gel injected subcutaneously mice at a 7-day-interval. Immune splenocytes myeloma cells SP2/0-Ag14 fused by polyethylene glycol 1450. Cell...
Objective of study is to prepare hybridomas-producers monoclonal antibodies brucellosis agent antigens. Materials and methods. B. abortus, melitensis, suis strains from the State collection microorganisms 48th Central Research Institute Affiliated Branch BALB/c mice. Hybridization was performed as described by G.Kohler C.Milstein in modification Fazekas De St. Scheidegger D. The specific activity immune sera, hybridoma supernatants, ascites fluid, antibody preparations using ELISA. Results...
Objective of the study was development immune-enzymatic monoclonal test-kit for detecting glanders and melioidosis agents. Materials methods. We used microbial cultures hybrid cell lines obtained from collection «48 th Central Research Institute» Ministry Defense Russian Federation. Hybridoma cells were incubated in peritoneal cavity BALB/c mice. Preparations antibodies isolated ascetic fluids through precipitation with ammonium sulfate purification by means ion-exchange chromatography....
Objective of the study was development experimental ELISA tests and lateral flow immunoassays for detection staphylococcal enterotoxins, A B types. Materials methods . Hybridomas, producing monoclonal antibodies against enterotoxins from State Collection Affiliated Branch “48th Central Research Institute” Ministry Defense Russian Federation, BALB/c mice were used in research. Hybridoma cells incubated culture flasks peritoneal cavity mice. Monoclonal isolated ascitic fluids through...
Objective of the study was to develop enzyme-immunoassay test-kit for detection Bacillus anthracis spores. Materials and methods . Microbial cultures from State Collection Microorganisms at premises Affiliated Branch «48 th Central Research Institute» Ministry Defense Russian Federation BALB/c mice were used in research. Hybridization B-lymphocytes with SP2/0-Ag14 myeloma cells performed according G. Kohler C. Milstein procedure De St. Fazekas P. Scheidegger modification. Hybridomas cultured...
Aim. Obtaining hybridomas, stable producing specific monoclonal antibodies against Burkholderia mallei and pseudomallei antigens. Materials methods. The microbial cultures from State Collection of Microorganisms the Branch 48 CSRI Defense Ministry Russian Federation (Kirov) BALB/c mouse were used in research. Hybridization B lymphocytes with SP2/0-Ag14 myeloma cells was performed by G.Kohler C.Milstein procedure De St. Fazekas D.Scheidegger modification. activity immune sera, hybridoma...
Objective of the study was to develop monoclonal immunoassay for detection pseudotuberculosis agent, serogroup I. Materials and methods. Specific components, that were used immune-chromatographic test-system development mouse antibodies hybrid cell lines, obtained lipopolysaccharide antigen outer membrane pathogen's «cold» variant (YP-101N2V4, YP-105S5A10); rabbit anti-species against murine immunoglobulins. Particles, (30±2) nm in diameter, prepare colloidal gold-antibody conjugate....
Carried out have been two experimental studies on hybridization of mouse myeloma cells and lymphocytes BALB/c mice immunized with inactivated microbe Francisella tularensis cultures. As a result obtained hybridomas-producers monoclonal antibodies (MAb) specific to the antigens tularemia agent. Evaluated prospects its application for detection agent under discussion using enzyme-linked immunoassay. Established is fact that produced by 31G1F10, 32E5D3, 35B11C8, 36C2F11 hybridomas make it...
Plant toxins – ricin and abrin, obtained in a purified form from the beans of castor bean Abrus precatorius respectively, are considered by Western experts as potential damaging agents biological nature. The purpose this work is to consider danger using abrin terrorism, well assess existing approaches means for identifying these toxins, treating intoxication caused them, level development vaccine preparations. Both have similar molecular structure mechanism action. They consist two subunits...
Introduction. The aim of the work was development enzyme immunoassay for detecting I and II types shiga-like toxins assessment it diagnostic properties. Materials methods. For research, we used hybridomas producing monoclonal antibodies to II, obtained at branch Federal State Budgetary Institution “48 Central Research Institute” Ministry Defense Russian Federation (Kirov); BALB/c mice; II. Hybridoma cells were cultured in culture flasks peritoneal cavity mice. Monoclonal isolated from...
Objective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I II types shiga-like toxins. Materials methods. Shiga-like toxins obtained in “48thCentral Research Institute” Ministry Defense Russian Federation (Kirov), BALB/c mice, myeloma cells SP2/0-Ag14 were used research. Immune splenocytes fused according to G. Kohler C. Milstein method De St. Fazekas D. Scheidegger modifcation using 50 % polyethylene glycol. Hybrid specifc cloned by limited...