A5035784444- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Mycobacterium research and diagnosis
- SARS-CoV-2 detection and testing
- Infections and bacterial resistance
- Pharmaceutical and Antibiotic Environmental Impacts
- Genomics and Phylogenetic Studies
- Enterobacteriaceae and Cronobacter Research
- COVID-19 Impact on Reproduction
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- COVID-19 Clinical Research Studies
- Parasites and Host Interactions
- Viral gastroenteritis research and epidemiology
- Renal cell carcinoma treatment
- Pneumonia and Respiratory Infections
- Pneumocystis jirovecii pneumonia detection and treatment
- Clostridium difficile and Clostridium perfringens research
- Reproductive tract infections research
University of Florence
2016-2025
Don Carlo Gnocchi Foundation
2024-2025
Marconi University
2020-2025
Azienda Ospedaliero-Universitaria Careggi
2020-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2024
University of Siena
2012-2017
Ospedale Santa Maria alle Scotte
2015-2017
IMT School for Advanced Studies Lucca
2005
BACKGROUND. Coronavirus disease 19 (COVID-19) is an emerging infectious caused by SARS-CoV-2. Antiviral immune response crucial to achieve pathogen clearance; however, in some patients excessive and aberrant host can lead acute respiratory distress syndrome. The comprehension of the mechanisms that regulate elimination, immunity, pathology essential better characterize progression widen spectrum therapeutic options.
To characterize a novel phenicol-oxazolidinone-tetracycline resistance gene, named poxtA, identified in previously described MRSA strain that was highly resistant to linezolid and also carried the cfr gene.The poxtA gene by bioinformatic analysis of whole genome sequence Staphylococcus aureus AOUC-0915. The cloned shuttle plasmid vector expressed Escherichia coli, S. Enterococcus faecalis investigate protein function. Comparative analyses at genetic levels were out using standard...
How is the semen quality of sexually active men following recovery from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection?Twenty-five percent with recent SARS-Cov-2 infections and proven healing were oligo-crypto-azoospermic, despite absence virus RNA in semen.The presence SARS-CoV-2 human its role contagion after disease 2019 (COVID-19) still unclear. So far, studies evaluating occurrence infected or recovered are scarce included a limited number participants.A...
Many term and preterm infants are commonly supplemented with probiotics to prevent adverse effects of antibiotic administration necrotizing enterocolitis they believed be safe. However, the supplementation <i>Lactobacillus rhamnosus</i> GG has been associated development sepsis a cause–effect relationship in six newborns children. In this study, we report two further cases discuss emerging issue probiotic safety neonates. We conclude that physicians must aware <i>L. can cause high-risk...
Although accumulating data have investigated the effect of SARS-CoV-2 mutations on antibody neutralizing activity, less is known about T cell immunity. In this work, we found that ancestral (Wuhan strain) Spike protein can efficaciously reactivate CD4+ memory in subjects with previous Alpha variant infection. This finding has practical implications, as many countries only one vaccine dose currently administered to individuals COVID-19, independently which was responsible We also a minority...
Limited data about New Delhi metallo-β-lactamase (NDM) bacteremia are available. Blood isolates from 40 patients with NDM were studied for antibiotic susceptibility and whole-genomic sequencing. has high 30-day mortality. In most cases, aztreonam-avibactam is active in vitro. Ceftazidime-avibactam plus aztreonam may represent a feasible therapeutic option.
Previous studies showed that the epidemic of carbapenem-resistant Klebsiella pneumoniae (CR-KP) observed in Italy since 2010 was sustained mostly by strains clonal group (CG) 258 producing KPC-type carbapenemases. In framework National Antibiotic-Resistance Surveillance (AR-ISS), a countrywide survey conducted 2016 to explore evolution phenotypic and genotypic characteristics CR-KP isolates.From March July 2016, hospital laboratories participating AR-ISS were requested provide consecutive,...
In the near future, overlap of Coronavirus disease 2019 (COVID-19) and dengue epidemics is a concrete threat in tropical regions. Co-epidemics COVID-19 could be an overwhelming challenge for health systems low- middle-income countries. this work, we investigated potential serological cross-reactions between patients. Among 32 positive sera, no Dengue virus (DENV) IgG/IgM results were observed. On other hand, one false-positive result was observed among 44 DENV-positive sera tested antibodies...
Persistent infection with High Risk-Human Papilloma Viruses (HR-HPVs) is a primary cause of cervical cancer worldwide. Vaginal-dysbiosis-associated bacteria were correlated the persistence HR-HPVs and increased risk. We obtained strains most represented bacterial species in vaginal microbiota evaluated their effects on survival epithelial cells immune homeostasis. The contribution each to supporting antiviral response was also studied. Epithelial cell viability affected by culture...
A nosocomial outbreak by cefiderocol (FDC)-resistant NDM-1-producing Klebsiella pneumoniae (NDM-Kp) occurred in a large tertiary care hospital from August 2021–June 2022 Florence, Italy, an area where NDM-Kp strains have become endemic. Retrospective analysis of cases observed January revealed that 21/52 were FDC-resistant. The was mostly sustained clonal expansion mutant with inactivated cirA siderophore receptor gene, which exhibited high-level resistance to FDC (MIC ≥ 32 mg/L) and spread...
Abstract Background Cefiderocol is a catechol-substituted cephalosporin with potent in vitro activity against carbapenem-resistant (CR) Gram-negative bacteria (GNB). susceptibility testing complex because iron concentrations need to be taken into consideration. Here, we assessed the clinical performance of Bruker’s UMIC® and corresponding iron-depleted CAMHB determine MIC by broth microdilution (BMD) for clinically relevant GNB. Methods MICs cefiderocol 283 GN isolates were determined BMD...
Vaginal dysbiosis is characterized by a decrease in the relative abundance of Lactobacillus species favor other species. This condition facilitates infections sexually transmitted pathogens including high risk (HR)-human papilloma viruses (HPVs) involved development cervical cancer. Some vaginal bacteria contribute to neoplastic progression inducing chronic inflammation and directly activating molecular pathways carcinogenesis. In this study, SiHa cells, an HPV-16-transformed epithelial cell...
Extended-spectrum beta-lactamases (ESBLs), AmpC-type (ACBLs) and carbapenemases are among the most important resistance mechanisms in Enterobacteriaceae . This study investigated presence of these consecutive non-replicate isolates Escherichia coli (n = 2,352), Klebsiella pneumoniae 697), Proteus mirabilis 275) from an Italian nationwide cross-sectional survey carried out October 2013. Overall, 15.3% were non-susceptible to extended-spectrum cephalosporins but susceptible carbapenems...
Enterococcus faecium E35048, a bloodstream isolate from Italy, was the first strain where oxazolidinone resistance gene optrA detected outside China. The also positive for cfr. WGS analysis revealed that two genes were linked (23.1 kb apart), being co-carried by 41,816-bp plasmid named pE35048-oc. This carried macrolide erm(B) and backbone related to of well-known faecalis pRE25 (identity 96%, coverage 65%). context original, part composite transposon, Tn6628, which integrated into encoding...
One hundred forty-five florfenicol-resistant enterococci, isolated from swine fecal samples collected 76 pig farms, were investigated for the presence of optrA, cfr, and poxtA genes by PCR. Thirty Enterococcus isolates had at least one linezolid resistance gene. optrA was found to be most widespread gene (23/30), while cfr detected in 6/30 7/30 enterococcal isolates, respectively. WGS analysis also showed cfr(D) faecalis (n = 2 isolates) avium 1 isolate). The hybridized both on chromosome...