A5009967655- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Pneumocystis jirovecii pneumonia detection and treatment
- Peptidase Inhibition and Analysis
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Antimicrobial Peptides and Activities
- Polyamine Metabolism and Applications
- Aquaculture disease management and microbiota
- Plant-Microbe Interactions and Immunity
- Fungal Biology and Applications
- Infectious Diseases and Mycology
- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Molecular Research
- Carbohydrate Chemistry and Synthesis
- Marine Bivalve and Aquaculture Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Natural Products and Biosynthesis
- Ruminant Nutrition and Digestive Physiology
- Microbial Metabolic Engineering and Bioproduction
- Fungal Plant Pathogen Control
- Vibrio bacteria research studies
- Enzyme-mediated dye degradation
Université de Bordeaux
2012-2024
Centre National de la Recherche Scientifique
2012-2024
Institut Polytechnique de Bordeaux
2012-2024
Laboratoire de Microbiologie Fondamentale et Pathogénicité
2012-2024
Institut de Recherche en Informatique et Systèmes Aléatoires
2022
Université de Rennes
2022
Laboratoire d'Analyse et d'Architecture des Systèmes
2021
Université de Toulouse
2021
École Nationale Supérieure d'Art
2010
Laboratoire des Sciences de l'Ingénieur, de l'Informatique et de l'Imagerie
2006
We report here the isolation of two isoforms a novel cysteine‐rich peptide from haemocytes (isoform A 4.438 Da and B 4.562 Da) plasma A) mussel, Mytilus galloprovincialis . The molecules display antibacterial activity against gram‐positive bacteria, whereas only isoform is active fungus Fusarium oxysporum gram‐negative bacteria Escherichia coli D31. Complete sequences were determined by combination Edman degradation, mass spectrometry cDNA cloning using haemocyte library. mature molecules,...
Plasma from the mussel Mytilus galloprovincialis previously immunized by injecting them with bacteria contains several bactericidal proteins. One protein, MGD-1, was purified reverse-phase HPLC of supernatant acidified cell-free hemolymph. Its biological activity is directed against both gram-positive and gram-negative but it not cytotoxic towards human erythrocytes nor protozoa. As determined mass spectrometry, molecular MGD-1 4418 Da. Primary-structure analysis revealed 38 amino acids...
ABSTRACT In previous papers, we characterised 3 types of 4-kDa, cysteine-rich, cationic antimicrobial peptides: MGDs (for Mytilus galloprovincialis defensins), mytilins and myticins, which are abundant in the mussel hemocytes. present work, revealed a differential distribution cells expressing different genes. addition, using confocal electron microscopy, confirmed that defensins were partially located sub-types circulating hemocytes although peptides can be same cell, even granule. We also...
We describe the acquisition of flucytosine, azole, and caspofungin resistance in sequential Candida glabrata bloodstream isolates collected from a bone marrow transplant patient with clinical failure. Point mutations C. FUR1 (CgFUR1) CgFKS2 overexpression CgCDR1 CgCDR2 were observed resistant isolates.
Inactivation of the FCY2 (cytosine permease), FCY1 deaminase), and FUR1 (uracil phosphoribosyltransferase) genes in Candida lusitaniae produced two patterns resistance to flucytosine. Mutant fur1 demonstrated 5-fluorouracil, whereas mutants fcy1 fcy2 fluconazole presence subinhibitory flucytosine concentrations.
We developed a new in vitro model for multi-parameter characterization of the time course interaction Candida fungal cells with J774 murine macrophages and human neutrophils, based on use combined microscopy, fluorometry, flow cytometry viability assays. Using fluorochromes specific to phagocytes yeasts, we could accurately quantify various parameters simultaneously single infection experiment: at individual cell level, measured association phagocyte survival, monitored parallel overall...
The fungal cell wall contains glycoproteins that interact with the host immune system. In prominent pathogenic yeast Candida albicans, Pmr1 acts as a Golgi-resident ion pump provides cofactors to mannosyltransferases, regulating synthesis of mannans attached glycoproteins. To gain insight into putative conservation such crucial process within opportunistic yeasts, we were particularly interested in studying role PMR1 homolog low-virulent species rarely causes candidiasis, guilliermondii. We...
The aim of this work was to elucidate the molecular mechanisms flucytosine (5FC) resistance and 5FC/fluconazole (FLC) cross-resistance in 11 genetically epidemiologically unrelated clinical isolates Candida lusitaniae. We first showed that levels transcription FCY2 gene encoding purine-cytosine permease (PCP) were similar wild-type strain, 6936. Nucleotide sequencing alleles revealed 5FC 5FC/FLC could be correlated with a cytosine-to-thymine substitution at nucleotide 505 fcy2 genes seven...
The pathogenic yeast Candida albicans is both a powerful commensal and pathogen of humans that can infect wide range organs body sites. Metabolic flexibility promotes infection colonization by this opportunistic pathogen. Yeast cell survival depends upon assimilation fermentable non-fermentable locally available carbon sources. Physiologically relevant sugars like glucose fructose are present at low levels in host niches. However, because the preferred substrate for energy biosynthesis...
Abstract The ability of intracellular pathogens to cause infection is related their capacity survive and grow inside macrophages or in other cell types. Candida albicans latent virulence likely be a similar mechanism avoiding killing by specialized cells the resulting such hostile environments. Using differential display reverse transcription polymerase chain reaction technique, we have identified seven genes induced C. during macrophage phagocytosis. Sequence analyses database searches...
Caspofungin is an echinocandin antifungal that inhibits glucan synthesis in the fungal cell wall. A Candida parapsilosis bloodstream isolate resistant to echinocandins was recovered from a patient who had undergone allogeneic hematopoietic stem transplantation. The FKS1 gene, encoding target synthase, contained heterozygous mutation resulting I1380T amino acid change, addition naturally occurring P660A polymorphism. When expressed at equivalent position Fks1p protein of C. lusitaniae, P642A...
A killed, whole-cell vaccine was produced to induce immunity in dogs against leptospirosis. The vaccine, containing serovar Copenhageni, and administered 12 beagle at both 8 weeks of age. Ten unvaccinated the same age group served as control group. live, virulent inoculum Leptospira (1.52 x 109 - 4.40 leptospires per dog) used challenge 2 (Study 1) 14 months 2) post-booster vaccination. At regular intervals, pre- post-challenge (PC), Microscopic Agglutination Test (MAT) performed measure...
Mussel (Mytilus galloprovincialis) plasma contains cytotoxic activity against both vertebrate (erythrocytes and mouse tumour) protozoan cells. Procaryotes (Escherichia coli Vibrio alginolyticus) were not sensitive to the cytotoxicity. The was still present in dialyzed samples but inhibited by heating at 45 °C. Large individual variability which correlated with protein concentration an increasing number of reactive specimens following injection, suggested that naive mussels various stages...
In a previous work, we described the possible relationship between defect of purine-cytosine permease and acquisition cross-resistance to antifungal combination flucytosine (5FC) fluconazole (FLC) in Candida lusitaniae (T. Noël, F. François, P. Paumard, C. Chastin, D. Brethes, J. Villard, Antimicrob. Agents Chemother. 47:1275-1284, 2003). Using degenerate PCR chromosome walking, cloned two FCY2-like genes lusitaniae. Northern blot analysis revealed that only one gene was expressed; it named...
Candida guilliermondii is an opportunistic emerging fungal agent of candidiasis often associated with oncology patients. This yeast also remains interesting biotechnological model for the industrial production value-added metabolites. The recent whole-genome sequencing C. ATCC 6260 reference strain provides resource elucidating new molecular events supporting pathogenicity, antifungal resistance and exploring potential metabolic engineering. In present study, we designed efficient...
AFM was combined to vibrational spectroscopy decipher morphological, mechanical and biochemical changes induced by caspofungin treatment on<italic>Candida</italic>.
ABSTRACT An unusual interaction between flucytosine and fluconazole was observed when a collection of 60 Candida lusitaniae clinical isolates screened for cross-resistance. Among eight resistant to (MIC ≥ 128 μg/ml) susceptible (0.5 < MIC 2 μg/ml), four became flucytosine-fluconazole cross both antifungals were used simultaneously. Fluconazole resistance occurred only in the presence high concentrations, higher concentration used, greater necessary trigger When flucytosine-...
Abstract The study of fruiting in the basidiomycete Agrocybe aegerita has shown that some haploid homokaryotic strains can spontaneously switch their mating specificities at two unlinked A and B type factors. This event causes dikaryotisation primary homokaryons without plasmogamy leads to differentiation sporulating fruit-bodies (pseudo-homokaryotic fruiting). For each factor, genetic analyses have revealed that: (1) parental switched types segregate meiotically as Mendelian markers, (2) a...