A5104667887- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Erythropoietin and Anemia Treatment
- T-cell and Retrovirus Studies
- Immune Response and Inflammation
- Gastric Cancer Management and Outcomes
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- Animal Disease Management and Epidemiology
- Influenza Virus Research Studies
- Vector-Borne Animal Diseases
- CRISPR and Genetic Engineering
- Pharmacological Effects of Natural Compounds
- Paleontology and Stratigraphy of Fossils
- Epigenetics and DNA Methylation
- Toxin Mechanisms and Immunotoxins
- Geological and Geophysical Studies
- Acute Myeloid Leukemia Research
- Electrolyte and hormonal disorders
- Acute Lymphoblastic Leukemia research
- Helicobacter pylori-related gastroenterology studies
- Monoclonal and Polyclonal Antibodies Research
- Hemoglobinopathies and Related Disorders
- interferon and immune responses
- Glycosylation and Glycoproteins Research
Kitasato University Medical Center
2015-2024
Kitasato University
1988-2020
Sapporo Medical University
1993-2019
National Sagamihara Hospital
2017
University of Occupational and Environmental Health Japan
2017
Med Center
2015
The University of Tokyo
2004-2008
Astronomy and Space
2008
Ehime University
1995-2006
Moi University
2004
Cells of human leukemic T-cell line Molt-4 which were cloned in soft agarose from individual colonies analyzed for the cytopathic effect and viral antigen expression after lymphotropic virus type III infection. The induction antigens by infection varied significantly among different cell clones. These clones did not show significant differences amount OKT-4 antigen, a possible surface receptor acquired immunodeficiency syndrome virus. Moreover, virus-producing ability susceptibility to each...
The clonal composition of EBV-infected cells was examined in three cases EBV-associated hemophagocytic syndrome by analysis the heterogeneity terminal repetitive sequences EBV genome, indicating monoclonal expansion all cases. Involvement T lymphoid determined situ hybridization using 35S-labeled RNA probes specific for small EBV-encoded nuclear RNAs, EBER1 and EBER2, combination with immunostaining TCR-beta chain, CD45RO, CD20, CD30 CD68 antigens these majority showing EBER transcripts were...
The ability to sense temperature is essential for organism survival and efficient metabolism. Body temperatures profoundly affect many physiological functions, including immunity. Transient receptor potential melastatin 2 (TRPM2) a thermosensitive, Ca(2+)-permeable cation channel expressed in wide range of immunocytes. TRPM2 activated by adenosine diphosphate ribose hydrogen peroxide (H(2)O(2)), although the activation mechanism H(2)O(2) not well understood. Here we report unique which...
We have previously shown evidence for the early recruitment of gamma delta T cells during disease course primary infections with Listeria monocytogenes or Salmonella choleraesuis in mice. Since at this stage do not produce IL-2, growth factor remains unknown. IL-15 is a novel cytokine that uses beta- and gamma-chain IL-2R signal transduction, produced by activated monocytes/macrophages. In study, we investigated proliferative activity appearing after infection S. 31N-1. The cells, which...
We investigated immunohistochemical localization of V2 vasopressin receptor along the nephron using a specific polyclonal antibody. Staining was observed in some thick ascending limbs and all principal inner medullary collecting duct (IMCD) cells. Not only basolateral but also luminal membrane stained ducts, especially terminal IMCD (tIMCD). To learn functional role tIMCD, we studied effects arginine (AVP) on osmotic water permeability (Pf), urea (Pu), cAMP accumulation isolated perfused rat...
The effects of exogenous cAMP, dibutyryl cAMP (DBcAMP) on LPS-induced liver injury were examined in mice made hypersensitive to LPS by treatment with i.v. injection Propionibacterium acnes. In vivo administration DBcAMP significantly protected P. acnes-treated from injury, including apoptosis hepatocytes. increased circulating IL-10 level correlation suppression the TNF-alpha after challenge mice. Treatment anti-IL-10 mAb abrogated protective effect injury. Similar findings, addition vitro...
Yersinia pseudotuberculosis is an enteric pathogen that induces a variety of clinical symptoms, fever, scarlatiniform rash, diarrhea, vomiting, and arthritis. Characteristic histopathologic findings in Y. infection such as lymphoid hyperplasia, typically seen mesenteric lymph nodes, suggest the stimulation large proportion T lymphocytes may be involved pathogenesis this infection. In study, we assessed mitogenic activity culture supernatants isolates investigated mechanism by which these...
Abstract Present evidence suggests that a majority of murine CD3 + intraepithelial intestinal lymphocytes (IEL) are extrathymically derived T cells and these IEL phenotypically express the CD8 homodimer (CD8αα). Recently, − have been reported to recombination activating gene (RAG‐1), suggesting precursors αα exist on epithelium. To study in detail whether can develop into IEL, we analyzed subset found it be separated two subsets, namely IEL. We show (1) mostly small, non‐granular Pgp‐1 IL‐2R...
Precise stratigraphic analysis of Middle-Upper Permian and Lower Triassic sequence at Chaotian in northern Sichuan, China, identified two remarkable mass extinction horizons, one the (Guadalupian-Lopingian; G-L) boundary other Upper Permian-Lower (P-T) boundary. Across each boundaries, biodiversity declined sharply fusulinid, rugose coral, brachiopod, ammonite, conodont, radiolaria. Both boundaries are characterized by biohorizons, i.e., marked major pre-existing fauna first appearance...
Erythropoietin production has been reported to occur in the peritubular interstitial fibroblasts kidney. Since erythropoietin nephron is controversial, we reevaluated We examined mRNA expressions of and HIF PHD2 using high-sensitive situ hybridization system (ISH) protein expression immunohistochemistry further investigated mechanism by hypoxia vitro human liver hepatocell (HepG2) rat intercalated cell line (IN-IC cells). ISH mice showed proximal convoluted tubules (PCTs), distal (DCTs)...
To study the impact of epigenetic changes on biological functions, ability to manipulate status certain genomic regions artificially could be an indispensable technology. "Epigenome editing" techniques have gradually emerged that apply TALE or CRISPR/Cas9 technologies with various effector domains isolated from code writers erasers such as DNA methyltransferase, 5-methylcytosine oxidase, and histone modification enzymes. Here we demonstrate a recognizing major satellite, consisting repeated...
To assess cancer-testis antigens (CTAs) expression in gastric cancer patients and examined their associations with clinicopathological factors.Eighty-three were evaluated this study. Gastric specimens for the gene of CTAs, Kitakyushu lung antigen-1 (KK-LC-1), melanoma antigen (MAGE)-A1, MAGE-A3 New York esophageal cancer-1 (NY-ESO-1), by reverse transcription PCR. Clinicopathological background information, such as gender, age, tumor size, macroscopic type, histology, depth invasion, lymph...
Activity-guided fractionations of leaf extracts from Laurus nobilis L. led to the isolation a known sesquiterpene lactone, deacetyl laurenobiolide (1). Compound 1 showed antimicrobial activity against periopathic pathogens (Actinomyces viscosus, Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans), opportunistic Gram-positive bacteria (Staphylococcus aureus Streptococcus pyogenes) fungi (Candida albicans, Cryptococcus neoformans Aspergillus fumigatus)....
Intestinal microbiotas of human subjects and effect antibiotic treatment on them have been reported with cultivation independent methods. However, Japanese fecal not studied enough. We constructed a clone library method to obtain results within 3 d. In this study, intestinal 29 healthy adults, whose samples were collected twice at 5 month intervals from each subject, analyzed our method, using those data as benchmark was evaluated. The fifty-eight assessed based percentages genus level, the...
Abstract Influenza virus (IFV) infection is a common cause of severe viral pneumonia associated with acute respiratory distress syndrome (ARDS), which difficult to control general immunosuppressive therapy including corticosteroids due the unfavorable effect on replication. Studies have suggested that excessive activation innate immunity by IFV responsible for pathologies. In this study, we focused CARD9, signaling adaptor known regulate immune through multiple sensor proteins and...
The Capitanian (Late Guadalupian) Maokou Formation at Chaotian in northern Sichuan, South China, is composed mainly of shallow marine shelf carbonates deposited on the Tethyan side China. By detailed field mapping and scientific drilling, we newly found out unique fossil assemblages a sharp lithologic change upper part Formation. main (over 130 m thick) algal packstone with Wordian-Capitanian large-tested fusulines, rugose corals other sessile benthos, whereas Uppermost Member (13 black limy...