A5010706679- Venomous Animal Envenomation and Studies
- Healthcare and Venom Research
- Insect and Pesticide Research
- Ion channel regulation and function
- Parasitic infections in humans and animals
- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Research on Leishmaniasis Studies
- Microbial Inactivation Methods
- Antimicrobial Peptides and Activities
- vaccines and immunoinformatics approaches
- Congenital Anomalies and Fetal Surgery
- Listeria monocytogenes in Food Safety
- Parasitic Infections and Diagnostics
- Helminth infection and control
- Milk Quality and Mastitis in Dairy Cows
- Lipid Membrane Structure and Behavior
- Trypanosoma species research and implications
- Coccidia and coccidiosis research
- Beetle Biology and Toxicology Studies
- Biochemical and Structural Characterization
- Vector-borne infectious diseases
- Neurobiology and Insect Physiology Research
- Leprosy Research and Treatment
Instituto Biológico
2007-2024
Universidade Federal do Paraná
2004-2024
Universidade Estadual do Paraná
2018-2022
Secretaria da Saúde
2002-2021
Universidade Estadual Paulista (Unesp)
2021
Oklahoma State Department of Health
2012-2014
Bites by the brown spider (Loxosceles spp.) are an important health problem in South America, where three species predominate laeta, Loxosceles gaucho, intermedia). Brown bites (loxoscelism) induce a block of cutaneous necrosis and, less commonly, may cause fatal systemic poisoning. A variety controversial protocols used to treat loxoscelism, while treatment with antivenin is only venom specific treatment. Here we studied action as well response for through experimental study that simulates...
Studies were carried out on the recovery rate and cysticerci location in bovines experimentally infected with Taenia saginata eggs. Three calves of 6.5 months one 19 age orally 2 x 10(4) eggs saginata. A fifth calf served as control. After 90 days infection, animals slaughtered organs skeletal muscles inspected using a slicing technique every 5 mm. From four calves, 702 recovered, which 570 (81.2%) alive 132 (18.8%) degenerated. The ranged from 0.01 to 1.43% an average 0.88%. presented...
A chimeric protein was constructed expressing three epitopes of LiD1, a dermonecrotic toxin from the venom Loxosceles intermedia spider. This species is responsible for large number accidents involving spiders in Brazil. We demonstrated that (rCpLi) generated atoxic and antibodies previously developed rabbits against synthetic reactive with rCpLi ELISA immunoblot assays. The antibody response evaluated by we have detected an all immunized animals. Overlapping peptides covering amino acid...
Loxoscelism is the most important form of araneism in South America. The treatment these accidents uses heterologous antivenoms obtained from immunization production animals with crude loxoscelic venom. Due to scarcity this immunogen, new alternatives for its substitution antivenom are medical interest. In present work, three linear epitopes astacin-like metalloprotease 1 (LALP1) (SLGRGCTDFGTILHE, ENNTRTIGPFDYDSIMLYGAY and KLYKCPPVNPYPGGIRPYVNV) two hyaluronidase (LiHYAL)...
Background The diagnosis of leprosy is primarily based on clinical manifestations, and there no widely available laboratory test for the early detection this disease, which caused by Mycobacterium leprae. In fact, treatment are key elements to successful control leprosy. Methodology/Principal Findings Peptide ligands antibodies from patients were selected phage-displayed peptide libraries. Three sequences expressed reactive phage clones chemically synthesized. Serological assays that used...
The first infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes disease 2019 (COVID-19), occurred in December 2019. Within a single month, reached other countries, spreading rapid and generalized manner worldwide to cause COVID-19 pandemic. In Brazil, number of cases surpassed 38 million. This study was conducted produce antibodies against SARS-CoV-2 investigate immunogenic potential synthetic peptides containing partial sequences main proteins...
O presente trabalho teve como objetivo pesquisar a ocorrência de leishmaniose visceral em cães com sinais clínicos compatíveis, procedentes clínicas veterinárias das diferentes regiões do Estado Paraná e caso positivo verificar autoctonia dos mesmos.Dos animais suspeita clínica epidemiológica LV, foi coletada amostra sangue para realização hemograma, provas bioquímicas, sorologia, cultura parasito, PCR RAPD.Os portadores quadro clínico, febre, hiperqueratose, onicogrifose emagrecimento...
Abstract Background An early diagnostic test for detecting infection in leprosy is fundamental reducing patients’ sequelae. The currently used lepromin not adequate disease diagnosis and, so far, no antigen to be intradermoreaction has proved sensitive and specific that purpose. Aiming at identifying new reagents skin tests, candidate antigens were investigated. Methods Random peptide phage display libraries screened by using antibodies from patients order identify peptides as reagents....
An important step in the development of therapeutic antivenoms is pre-clinical testing using vivo methods to assess their neutralizing potency. For spider (Loxosceles species), horse serum potency against necrotizing activities Loxosceles intermedia crude venom currently tested rabbits. These procedures are time consuming and involve a large number animals. The aim this study was develop an vitro method anti-Loxosceles sera. We first demonstrated that it not possible establish correlation...
Phospholipases-D (PLDs) found in Loxosceles spiders’ venoms are responsible for the dermonecrosis triggered by envenomation. PLDs can also induce other local and systemic effects, such as massive inflammatory response, edema, hemolysis. Recombinant reproduce all of deleterious effects induced whole venoms. Herein, wild type mutant two species involved accidents—L. gaucho L. laeta—were recombinantly expressed characterized. The mutations related to amino acid residues relevant catalysis...
Um teste de ELISA indireto (ENZYME-LINKED IMMUNOSORBENT ASSAY) foi desenvolvido para pesquisa anticorpos contra-C. bovis em bovinos experimental e naturalmente infectados. Foram estudados três antígenos: antígeno parcial C. cellulosae, total longicollis. Na padronização do foram analisadas as seguintes combinações: 250 500ng proteína/cavidade, diluição dos soros 50, 100, 200 400 vezes, conjugado (IgG cabra anti-IgG bovina conjugada com peroxidase) 800 vezes. Do cruzamento das condições acima...
Loxosceles spider bites often lead to serious envenomings and no definite therapy has yet been established. In such a context, it is of interest consider an antibody-based targeted therapy. We have previously prepared murine monoclonal IgG (LiMab7) that binds 32–35 kDa components intermedia venom neutralizes the dermonecrotic activity venom. Here, we re-engineered LiMab7 into recombinant diabody. The protein was produced in bacteria then functionally characterized. It proved be efficient at...
Cystercosis is the most frequently detected pathology in post mortem inspection bovine slaughtered at establishments under Federal Inspection Service (SIF). In Brazil, average prevalence of this parasitosis 5%, but rates can vary according to anatomic area screened, geographic region, or period time work was carried out. order find out among bovine, State Parana-Brazil, a six months research – from July December, 2000 animals originating 137 towns and Argus Cold Store, located Metropolitan...
An NC-1 mimotope from Taenia solium cysticerci can help identify patients with neurocysticercosis through immunoassay. After chemical synthesis, an peptide was coupled to bovine serum albumin (NC-1/BSA) for used as immunogen in murine crassiceps cysticercosis, which is experimental model of cysticercosis caused by T. solium. NC-1/BSA immunisation decreased parasitaemia inducing 74% protection compared the 77% obtained crude antigen. The influence also observed on size and stage development...
Based on degradation of sphingomyelin/cholesterol liposomes containing entrapped horseradish peroxidase, we evaluated the Sphingomyelinase-D (SMase-D) activity scorpion, spider and snake venoms by monitoring spectrophotometrically product oxidation HRP released. The results indicate that Loxosceles crude (Loxosceles intermedia, laeta, gaucho similis) displayed SMase-D in a concentration-dependent manner. Furthermore, this was blocked anti-loxoscelic antivenom. However, Tityus serrulatus...