The aim of our study was to compare the total 25(OH)D fraction, bioavailable vitamin and free D fraction in spring fall a group healthy individuals.

Background: Autism spectrum disorder (ASD) is a developmental characterized by deficits in social interaction, restricted interest and repetitive behavior. Oxidative stress response to environmental exposure plays role virtually every human disease represents significant avenue of research into the etiology ASD. The aim this study was explore diagnostic utility four urinary biomarkers oxidative stress. Methods: One hundred thirty-nine (139) children adolescents with ASD (89% male, average...

Autism spectrum disorder (ASD) has been associated with disruptions in tryptophan (TRP) metabolism, affecting the production of key neuroactive metabolites. Investigating these metabolic pathways could yield valuable biomarkers for ASD severity and progression. We included 44 children healthy children, members same family. The average age group was 10.7 years, while control 9.4 years. Urinary metabolites were quantified via liquid chromatography-mass spectrometry operating multiple reaction...

Thymidine kinase (TK1) is an enzyme involved in DNA synthesis that leaks into the blood as a result of high cell turnover, particularly case cancer. Serum TK1 activity has been used for prognosis and monitoring leukemia lymphoma patients many years. Here, we describe first clinical results with newly developed TK 210 ELISA from AroCell AB. Sera 124 breast cancer known TNM classification along sera 53 healthy females were analyzed by protein levels 3[H]-deoxythymidine (dThd) phosphorylation...

To explore correlations between the sFlt-1/PlGF ratio and uterine arteries (UtA) Doppler indexes in placental dysfunction-related disorders (PDD).We prospectively included women with a singleton pregnancy preeclampsia (PE) only (n = 22), fetal growth restriction (FGR) 32), FGR 12), or normal 29).In PDDs, significantly positive mean UtA pulsatility (mPI-UtA), as well resistance index (mRI-UtA) were found (p 0.015, p 0.019, respectively), but not pregnancies. PDD signs of impaired...

Prostate-specific antigen (PSA) is an established tumour marker for prostate cancer (PCa). Serum thymidine kinase 1 a possible new the detection of PCa. The aim study was to investigate diagnostic value AroCell TK 210 enzyme-linked immunosorbent assay (ELISA) together with free PSA, [-2]proPSA, and Prostate Health Index (PHI) in differentiating PCa from benign urological conditions.Serum samples 140 patients PSA values range between 2 10 µg/L were collected at Ljubljana University Medical...

To evaluate changes in vascular function and serum biomarkers women with without preeclampsia (PE) to create a model for the easier more precise diagnosis of PE future.Endothelial arterial stiffness were evaluated using peripheral tonometry concentrations placental growth factor (PlGF), soluble fms like tyrosine kinase 1 (sFlt-1) endoglin (sEng) determined by immunoassay.Arterial deteriorates endothelial is better compared healthy pregnancy. Women who developed had decreased PlGF...

Although the levels of cardiac troponin I (cTnI) have proved to be a useful diagnostic biomarker acute myocardial infarction, there are wide variety point-of-care (POC) analysers, which provide measurements cTnI. The aim this study was compare results obtained by ADVIA Centaur ultra-assay cTnI assay (us-cTnI), high-sensitive (hs-cTnI) and POC high-sensitivity using PATHFAST. We also aimed explore total turnaround time (TAT) for laboratory PATHFAST analyser.Samples from 161 patients were...

Objectives—To conduct a secondary analysis of prediction accuracy biophysical markers for suspected Preeclampsia (PE), Fetal Growth Restriction (FGR) and the two combined near delivery in Slovenian cohort. Methods—This was database total 125 pregnant women attending high-risk pregnancy clinic due to PE (n = 31), FGR 16) + 42) from 28–39 weeks gestation their corresponding term 21) preterm (PTD, n 15) controls. Data Mean Arterial blood Pressure (MAP) Uterine artery pulsatility index (UtA PI)...

Metabolic disorders and nutritional deficiencies in ASD children may be identified by the determination of urinary-modified compounds. In this study, levels selected seven modified compounds: O-methylguanosine, 7-methylguanosine, 1-methyladenosine, 1-methylguanine, 7-methylguanine, 3-methyladenine, 8-hydroxy-2`-deoxyguanosine group 143 68 neurotypical controls were analyzed. An ancillary aim was to verify if reported differed depending on pathogenetic scoring (mild deficit, moderate severe...

Abstract Background: The role of oxidative stress in female reproduction is an area that needs more investigation. This study aims to assess the levels follicular fluid (FF) samples women undergoing vitro fertilization (IVF) and relate them different diagnoses. Methods: A total 199 woman were enrolled; 37 diagnosed with polycystic ovary syndrome (PCOS), 71 endometriosis 41 tubal factor infertility (TFI) 50 patients used as a control group. sample FF was collected from each patient at oocyte...

Oxidative stress in the follicular fluid (FF) is thought to be responsible for abnormal development of oocytes. In our study patients with polycystic ovarian syndrome (PCOS), endometriosis, and tubal infertility factor (TIF), healthy women a male infertility, were prospectively enrolled. From each patient, sample individual FF was collected from dominant follicle. Concentration levels TAS, 8-IP, 8-OHdG, AMH determined. PCOS, we found significantly lower values oxidative markers FF. 8-IP TAS...

Objective—the objective of this study was to assess the accuracy placental growth factor (PlGF), soluble Fms-like Tyrosine Kinase 1 (sFlt-1), and endoglin (sEng) in diagnosis suspected preeclampsia (PE) with without fetal restriction (FGR) near delivery. Methods—this is a secondary analysis dataset 125 pregnant women presenting at high risk pregnancy clinic PE, FGR or PE + University Medical Center Slovenia. The included 31 cases, 16 42 15 cases who developed unrelated complications before...

Objective: We previously provided evidence to confirm that maternal serum levels of soluble Fms-like tyrosine kinase-1 (sFlt-1), placental growth factor (PlGF), and their ratio are useful tools direct the management preeclampsia (PE), fetal restriction (FGR), PE+FGR near delivery. In this secondary analysis, we further examine potential additive value Inhibin-A, which is a hormone marker transforming family, accuracy by PlGF sFlt-1. Methods: conducted analysis where extracted data cohort 125...

Abstract Background 13 C-mixed triglyceride breath test ( C-MTGT) is a non-invasive for the detection of moderate and severe pancreatic exocrine insufficiency (PEI), but it requires prolonged sampling. The aim this study was to determine diagnostic power abbreviated C-MTGT in detecting PEI patients after subtotal total gastrectomy performed due gastric cancer. Subjects methods This cross-sectional observational included 3 groups subjects; healthy controls, with gastrectomy. Demographic...

Background. The α-fetoprotein receptor (RECAF) is a proposed novel tumor marker for detecting several different types of tumors, including prostate cancer (PCa).Objectives. aim the study was to evaluate RECAF in discriminating benign prostatic conditions from PCa and compare it with prostate-specific antigen (PSA).Material Methods. A total 64 patients elevated serum PSA levels and/or abnormal digital rectal examination referred tertiary center transrectal ultrasound (TRUS) biopsy were...

Abstract Background: Prostate-specific antigen (PSA) is an established tumor marker for the diagnosis of patients with prostate cancer. The aim study was to evaluate performance [-2]proenzyme PSA ([-2]proPSA) and health index (PHI) markers in differential between benign prostatic diseases Methods: Total (tPSA), free (fPSA) [-2]proPSA were measured using antibody -based sandwich enzyme-linked immunosorbent assay a chemiluminescent detection system 110 patients, tPSA 1.6–8.0 µg/L. PHI...

Autism spectrum disorder (ASD) has been associated with disruptions in tryptophan (TRP) metabolism, affecting the production of key neuroactive metabolites. Investigating these metabolic pathways could yield valuable biomarkers for ASD severity and progression. We included 44 children healthy controls, members same family. group average age was 10.7 years while control 9.4 years. Urinary metabolites were quantified via liquid chromatography - mass spectrometry operating multiple reaction...

Cardiac troponin I (cTnI) is a standard biomarker for the diagnosis of acute myocardial infarction (AMI). While older, ultra-sensitive cTnI (us-cTnI) assays use 99th percentile as reference threshold, newer high-sensitive (hs-cTnI) limit detection or functional sensitivity instead. However, little has been done to systematically compare these two methods. The present study also served validation hs-cTnI in our laboratory. Here, we compared results obtained from blood serum 8810 patients...

Disbalance balance between oxidants and antioxidants is called oxidative stress could be presented as index (OSI). OSI determined by the reactive oxygen metabolites (d-ROM test) to assess plasma antioxidant capacity test (PAT measure antioxidants. The aim of study was evaluate predictive value in disease COVID-19. d-ROMs results were highest SARS-CoV-2 POSITIVE group (365+/-112), lower NEGATIVE (314+/-72.4), lowest an INTENSIVE CARE UNIT (ICU) (277+/-142) U.Carr. PAT values (2762+/-387),...

Purpose: To determine whether high-dose intravenous vitamin C reduces oxidative stress in patients with severe preeclampsia the first days postpartum. Methods: Biomarkers of were assessed as secondary outcomes a single-center, randomized, placebo-controlled trial. Thirty-four singleton pregnancies complicated by features randomized into two groups: (1.5 g/6 h) (n = 17) or placebo 17). Urinary concentrations dityrosine, 8-hydroxy-2-deoxyguanosine (8-OHdg), 8-isoprostane, and N...

Fecal calprotectin is a biomarker for monitoring inflammatory bowel disease (IBD) activity. Our aim, therefore, was to evaluate two new assays, the point of care test Quantum Blue and Liaison Calprotectin with respect Calprest, commonly used assay, determine their performance IBD diagnosis.We included 73 prospective patients IBD. measured analysed routine Calprest assay recently introduced Calprotectin. Furthermore, we compared results by Bland Altman analysis, Passing-Bablok regression.We...

Abstract Background: Thymidine Kinase 1 (TK1) is a cytosolic enzyme that plays an important role in DNA precursor synthesis and TK1 activity serum established biomarker for prognosis treatment monitoring, particularly haematological malignancies. The AroCell TK 210 ELISA determines protein levels, contrast to activity, it has demonstrated superior sensitivity distinguishing between subjects with solid tumours controls. Pro PSA, free PSA the prostate health index (PHI) have been proposed as...