A5038924632- Bone Tissue Engineering Materials
- Dental Implant Techniques and Outcomes
- Orthopaedic implants and arthroplasty
- Mesenchymal stem cell research
- CAR-T cell therapy research
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Bone Metabolism and Diseases
- Periodontal Regeneration and Treatments
- Spine and Intervertebral Disc Pathology
- biodegradable polymer synthesis and properties
- Synthesis and Biological Evaluation
- Facial Trauma and Fracture Management
- Multiple Myeloma Research and Treatments
- Bone health and treatments
- Immune Cell Function and Interaction
- Spectroscopy Techniques in Biomedical and Chemical Research
- Dental materials and restorations
- Viral Infectious Diseases and Gene Expression in Insects
- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Osteoarthritis Treatment and Mechanisms
- Bone fractures and treatments
- Titanium Alloys Microstructure and Properties
Kuros Biosciences (Switzerland)
2019-2024
Queen Mary University of London
2014-2023
Bronkhorst (Netherlands)
2011-2023
Maastricht University
2023
University of Twente
2008-2022
University Medical Center Utrecht
1999-2020
Maastricht University Medical Centre
2019
Xpand Biotechnology (Netherlands)
2008-2017
Leiden University
1991-2005
Unilever (Netherlands)
1965-1973
Biomaterials can be endowed with biologically instructive properties by changing basic parameters such as elasticity and surface texture. However, translation from in vitro proof of concept to clinical application is largely missing. Porous calcium phosphate ceramics are used treat small bone defects but general do not induce stem cell differentiation, which essential for regenerating large defects. Here, we prepared varying physicochemical structural characteristics. Microporosity...
Human mesenchymal stem cells (MSCs) reside under hypoxic conditions in vivo, between 4% and 7% oxygen. Differentiation of MSCs results inhibited osteogenesis, while chondrogenesis is unaffected. The reasons for these may be associated with the inherent metabolism cells. present investigation measured oxygen consumption, glucose consumption lactate production during proliferation subsequent differentiation towards osteogenic chondrogenic lineages. expanded normoxia had an rate ∼98...
Targeting nonspecific, tumor-associated antigens (TAA) with chimeric antigen receptors (CAR) requires specific attention to restrict possible detrimental on-target/off-tumor effects. A reduced affinity may direct CAR-engineered T (CAR-T) cells tumor expressing high TAA levels while sparing low normal tissues. However, decreasing the of CAR-target binding compromise overall antitumor Here, we demonstrate prime importance type intracellular signaling on function low-affinity CAR-T cells.We...
Porous glass ceramic, which was prepared from Bioglass powder (45S5, U.S. Biomaterials) by foaming with diluted H(2)O(2) solution and sintering at 1000 degrees C for 2 h, implanted as cylinders (5 mm in diameter 6 length) thigh muscles of dogs 3 months. Histological observation made on thin un-decalcified sections. Bone formation histologically found pores all implants (X16) retrieved 16 dogs. The bone tissue also identified backscattered scanning electron microscopy (BSE) energy dispersive...
Titanium alloy implants were precoated biomimetically with a thin and dense layer of calcium phosphate then incubated either in supersaturated solution or phosphate-buffered saline, each containing bovine serum albumin (BSA) at various concentrations, under physiological conditions for 48 h. Coated underwent scanning electron microscopy, immunohistochemical evaluation, Fourier transform infrared spectroscopy, X-ray diffraction. The quantity BSA taken up by coatings the kinetics protein...
In this study we investigated the bone-forming capacity of tissue-engineered (TE) constructs implanted ectopically in goats. As cell survival is questionable large animal models, significance vitality, and thus whether living cells instead only potentially osteoinductive extracellular matrix are required to achieve bone formation. Vital TE porous hydroxyapatite (HA) covered with differentiated marrow stromal (BMSCs) within an (ECM) were compared identical that devitalized before...
At present, it is well known that populations of human bone marrow stromal cells (HBMSCs) can differentiate into osteoblasts and produce bone. However, the amount with osteogenic potential ultimately obtained will still be dependent on both patient physiological status culture system. In addition, to use a cell therapy approach in orthopedics, large numbers required and, as result, knowledge factors affecting growth kinetics these needed. present study we investigated effect dexamethasone...
ABSTRACT Since targeting of recombinant adenovirus vectors to defined cell types in vivo is a major challenge gene therapy and vaccinology, we explored the natural diversity human tissue tropism. Hereto, constructed library Ad5 carrying fibers from other serotypes. From this library, identified that efficiently infect cells are important for diverse approaches induction immunity. For several medical applications (prenatal diagnosis, artificial bone, vaccination, cardiovascular disease),...
Most therapeutic applications of bone marrow stromal cells (MSCs), or mesenchymal stem cells, require expansion these cells. This study aimed to obtain more information about human MSCs regarding their characteristics: growth, metabolism, and growth inhibitors. In addition, the same factors were examined for (model species) goat rat evaluate differences between mammalian species. MSC proliferation, nutrient consumption, metabolite production determined five donors per inhibitory...
During the past decade, there has been much interest in use of human mesenchymal stem cells (hMSCs) bone tissue engineering. HMSCs can be obtained relatively easily and expanded rapidly culture, but for clinical purposes large numbers are often needed cost should kept to a minimum. A rapid efficient culturing protocol would therefore beneficial. In this study, we examined effect different medium compositions on expansion osteogenic differentiation marrow-derived hMSCs from 19 donors. We also...
Material-induced bone formation reported in canine, bovid, suid, and primate species does not often occur lagomorph or rodent models. In this study, we test biphasic calcium phosphate hydroxyapatite- induced subcutaneous pockets of mice intramuscular rats, rabbits, dogs. All scaffolds are similar size, all animals were sacrificed at 90 days post-implantation. dogs (N = 8), implants showed with significantly more formed phosphates (30 +/- 6%, N 8) as compared to hydroxyapatite (14 5%, (p...
Adoptive transfer of chimeric antigen receptor-transduced T cells is a promising strategy for cancer immunotherapy. The CD38 molecule, with its high expression on multiple myeloma cells, appears suitable target antibody therapy. Prompted by this, we used three different sequences to generate second-generation retroviral CD38-chimeric receptor constructs which transduced from healthy donors and patients. We then evaluated the preclinical efficacy safety cells. Irrespective donor sequence,...
For the continuous and fast expansion of mesenchymal stem cells (MSCs), microcarriers have gained increasing interest. The aim this study was to evaluate growth metabolism profiles MSCs, expanded in a microcarrier-based cultivation system. We investigated various conditions expand goat on Cytodex 1 microcarriers. These differed feeding regime, i.e. addition fresh proliferation medium, with or without new all conditions, cell attachment, proliferation, energy source consumption, metabolite...