A5017437663- Ophthalmology and Visual Impairment Studies
- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Optical Coherence Tomography Applications
- Retinal Development and Disorders
- Lysosomal Storage Disorders Research
- Neural Networks and Applications
- Glycogen Storage Diseases and Myoclonus
- Machine Learning in Healthcare
- Machine Learning and Data Classification
- Protein Tyrosine Phosphatases
- Spectroscopy and Chemometric Analyses
- Bat Biology and Ecology Studies
- Remote Sensing and Land Use
- Remote Sensing in Agriculture
- melanin and skin pigmentation
- Ophthalmology and Visual Health Research
- Surface Roughness and Optical Measurements
- Parkinson's Disease and Spinal Disorders
- Fuzzy Logic and Control Systems
- Animal Ecology and Behavior Studies
- Yersinia bacterium, plague, ectoparasites research
- Industrial Vision Systems and Defect Detection
- Optical Network Technologies
Medical College of Wisconsin
2018-2025
Optical coherence tomography (OCT) allows noninvasive visualization of individual retinal layers and has become a mainstay in the diagnosis management wide range systemic diseases. As number available treatments increases, there is growing interest developing sensitive OCT-based biomarkers for assessing therapeutic response. In particular, hyperreflective outer band just posterior to external limiting membrane, also known as ellipsoid zone (EZ), widely used biomarker photoreceptor structure....
Directional optical coherence tomography (D-OCT) allows the visualization of Henle fiber layer (HFL) in vivo. Here, we used D-OCT to characterize HFL and outer nuclear (ONL) albinism examine relationship between true foveal ONL peak cone density.Horizontal B-scans were acquired, registered, averaged for 12 subjects with oculocutaneous 26 control subjects. Averaged images manually segmented extract thickness. Adaptive optics scanning light ophthalmoscopy was acquire mosaic 10 albinism, from...
The foveal cone mosaic can be directly visualized using adaptive optics scanning light ophthalmoscopy (AOSLO). Previous studies in individuals with normal vision report wide variability the topography of mosaic, especially value peak density (PCD). While these often involve a human grader, there have been no examining intergrader reproducibility metrics. Here we re-analyzed published AOSLO images from 44 to assess relationship between centroid (CDC) location and PCD. Across 5 graders...
Purpose: Achromatopsia (ACHM) is an autosomal recessive retinal disease associated with reduced or absent cone function. There debate regarding the extent to which structure shows progressive degeneration in patients ACHM. Here, we used optical coherence tomography (OCT) images evaluate outer nuclear layer (ONL) thickness and ellipsoid zone (EZ) integrity over time individuals Methods: Sixty-three genetically confirmed ACHM follow-up ranging from about 6 months 10 years were imaged using...
Using adaptive optics (AO), it is possible to deliver near diffraction-limited stimuli the human retina assess relationship between cone mosaic and visual function. Here we sought establish device-specific control data for future studies of individuals with retinal disease. We used AOSLO quantify measure acuity in dominant eye 18 (7M, 11F; 15-67 years) without pathology. Average density at centroid was 186,925 cones/mm^2. Visual assessed using an AO-corrected Snellen E presented via a...
The use of “quality” to describe the usefulness an image is ubiquitous but often subject domain specific constraints. Despite its continued as imaging modality, adaptive optics scanning light ophthalmoscopy (AOSLO) lacks a dedicated metric for quantifying quality photoreceptors. Here, we present approach evaluating that extracts estimate signal noise ratio. We evaluated performance in 528 images photoreceptors from two AOSLOs, modalities, and healthy or diseased retinas. algorithm was...
To mitigate the substantial post-processing burden associated with adaptive optics scanning light ophthalmoscopy (AOSLO), we have developed an open-source, automated AOSLO image processing pipeline both “live” and “full” modes. The live mode provides feedback during acquisition, while full is intended to automatically integrate copious disparate modules currently used in generating analyzable montages. mean (±SD) lag between initiation montage placement for was 54.6 ± 32.7s. reduced overall...
Purpose: To compare cone mosaic metrics derived from adaptive optics scanning light ophthalmoscopy (AOSLO) images with those Heidelberg Engineering SPECTRALIS High Magnification Module (HMM) images. Methods: Participants contiguous mosaics had HMM imaging performed at locations superior and temporal to the fovea. These were registered averaged offline then aligned split-detection AOSLO images; 200 × 200-µm regions of interest extracted both modalities. Cones semi-automatically identified by...
Quantification of the rod photoreceptor mosaic using adaptive optics scanning light ophthalmoscopy (AOSLO) remains challenging. Here we demonstrate a method for deriving estimates density and rod:cone ratio based on measures spacing, cone numerosity, inner segment area. Twenty-two AOSLO images with complete visualization were used to validate this spacing-derived estimating density. The was then estimate metrics in an additional 105 without visualization. comparable published data from...
Background The 13-lined ground squirrel (13-LGS) possesses a cone-dominant retina that is highly amenable to non-invasive high-resolution retinal imaging. ability for longitudinal assessment of photoreceptor mosaic with an adaptive optics scanning light ophthalmoscope (AOSLO) has positioned the 13-LGS become accessible model vision research. Here, we examine interocular symmetry, repeatability, and reliability cone density measurements in 13-LGS. Methods Thirteen (18 eyes) were imaged along...
Lafora disease is a genetic neurodegenerative metabolic disorder caused by insoluble polyglucosan aggregate accumulation throughout the central nervous system and body. The retina an accessible neural tissue, which may offer alternative methods to assess neurological diseases quickly noninvasively. In this way, noninvasive imaging provide means characterize disease, enables earlier identification diagnosis of ability monitor progression. study, we sought individuals with using non-invasive...
The adaptive optics scanning light ophthalmoscope (AOSLO) allows cellular resolution imaging of the living retina. accuracy many quantitative measurements made from these images requires accurate estimates lateral scale images. Here, we used trial lenses, which are known to affect relative magnification retinal image, compare empirical measures image with theoretical a four-surface optical model. model overestimated empirically determined change in 70% subjects examined, albeit varying...
Purpose To compare peak cone density predicted from outer segment length measured on optical coherence tomography with direct measures of adaptive optics scanning light ophthalmoscopy.