A5066053426- Photoacoustic and Ultrasonic Imaging
- Nanoplatforms for cancer theranostics
- Optical Imaging and Spectroscopy Techniques
- Photodynamic Therapy Research Studies
- Thermography and Photoacoustic Techniques
- Esophageal Cancer Research and Treatment
- Spectroscopy Techniques in Biomedical and Chemical Research
- CCD and CMOS Imaging Sensors
- Optical Coherence Tomography Applications
- Advanced NMR Techniques and Applications
- Infrared Thermography in Medicine
- Particle Detector Development and Performance
- Advanced X-ray and CT Imaging
- Biosensors and Analytical Detection
- Electron Spin Resonance Studies
- Radiomics and Machine Learning in Medical Imaging
- Medical Imaging Techniques and Applications
- Advanced MRI Techniques and Applications
- Extracellular vesicles in disease
- Advanced Fluorescence Microscopy Techniques
- Gastric Cancer Management and Outcomes
- Advanced X-ray Imaging Techniques
- Angiogenesis and VEGF in Cancer
- Infrared Target Detection Methodologies
- Advanced Image Fusion Techniques
University of Cambridge
2016-2025
Cancer Research UK
2016-2025
Cancer Research UK Cambridge Center
2015-2024
University of Oxford
2023
California State University, Northridge
2023
Universitätsklinikum Erlangen
2022
Friedrich-Alexander-Universität Erlangen-Nürnberg
2022
Bridge University
2021
Google (United States)
2018
Stanford Medicine
2018
Dynamic nuclear polarization of (13)C-labeled cell substrates has been shown to massively increase their sensitivity detection in NMR experiments. The gain is sufficiently large that if these polarized molecules are injected intravenously, spatial distribution and subsequent conversion into other metabolites can be imaged. We have used this method image the fumarate malate a murine lymphoma tumor vivo after i.v. injection hyperpolarized [1,4-(13)C(2)]fumarate. In isolated cells, rate labeled...
Dynamic nuclear polarization (DNP) of (13)C-labeled metabolic substrates in vitro and their subsequent intravenous administration allow both the location hyperpolarized substrate dynamics its conversion into other products to be detected vivo. We report here hyperpolarization [1-(13)C]-ascorbic acid (AA) [1-(13)C]-dehydroascorbic (DHA), reduced oxidized forms vitamin C, respectively, evaluate performance as probes tumor redox state. Solution-state 10.5 ± 1.3% was achieved for at pH 3.2,...
The recent introduction of a dynamic nuclear polarisation technique has permitted noninvasive imaging tumour cell metabolism in vivo following intravenous administration 13C-labelled substrates. Changes hyperpolarised [1-13C]pyruvate and [1,4-13C2]fumarate were evaluated both MDA-MB-231 cells implanted tumours doxorubicin treatment. Treatment resulted the induction apoptosis, which was accompanied by decrease 13C label flux between lactate, correlated with cellular NAD(H) coenzyme pool....
Raman spectroscopy, amplified by surface enhanced scattering (SERS) nanoparticles, is a molecular imaging modality with ultra-high sensitivity and the unique ability to multiplex readouts from different targets using single wavelength of excitation. This approach holds exciting prospects for range applications in medicine, including identification characterization malignancy during endoscopy intraoperative image guidance surgical resection. The development SERS nanoparticles presently...
Abstract Purpose: To evaluate the potential of targeted photoacoustic imaging as a noninvasive method for detection follicular thyroid carcinoma. Experimental Design: We determined presence and activity two members matrix metalloproteinase family (MMP), MMP-2 MMP-9, suggested biomarkers malignant lesions, in FTC133 tumors subcutaneously implanted nude mice. The agent used to visualize was MMP-activatable probe, Alexa750-CXeeeeXPLGLAGrrrrrXK-BHQ3. Cleavage imaged after intratumoral...
Photoacoustic imaging combines the high contrast of optical with spatial resolution and penetration depth ultrasound. This technique holds tremendous potential for in small animals importantly, is clinically translatable. At present, there no accepted standard physical phantom that can be used to provide routine quality control performance evaluation photoacoustic instruments. With growing popularity advent several commercial animal systems, it important develop a strategy assessment such...
Acute kidney injury (AKI) is a common and important medical problem, affecting 10% of hospitalized patients, it associated with significant morbidity mortality. The most frequent cause AKI acute tubular necrosis (ATN). Current imaging techniques biomarkers do not allow ATN to be reliably differentiated from differential diagnoses, such as glomerulonephritis (GN). We investigated whether 13 C magnetic resonance spectroscopic (MRSI) might the noninvasive diagnosis ATN. MRSI hyperpolarized...
Abstract Hyperspectral imaging (HSI) enables visualisation of morphological and biochemical information, which could improve disease diagnostic accuracy. Unfortunately, the wide range image distortions that arise during flexible endoscopy in clinic have made integration HSI challenging. To address this challenge, we demonstrate a hyperspectral endoscope (HySE) simultaneously records intrinsically co-registered standard-of-care white light images, allows to be compensated computationally an...
Optoacoustic tomography is a fast developing imaging modality, combining the high contrast available from optical excitation of tissue with resolution and penetration depth ultrasound detection. Light subject to both absorption scattering when traveling through tissue; adequate knowledge properties hence spatial fluence distribution required create an optoacoustic image that directly proportional chromophore concentrations at all depths. Using data commercial multispectral (MSOT) system, we...
Poor oxygenation of solid tumours has been linked with resistance to chemo- and radio-therapy poor patient outcomes, hence non-invasive imaging oxygen supply demand in could improve disease staging therapeutic monitoring. Optoacoustic tomography (OT) is an emerging clinical modality that provides static images endogenous haemoglobin concentration oxygenation. Here, we demonstrate enhanced (OE)-OT, exploiting gas challenge visualise the spatiotemporal heterogeneity tumour vascular function....
Abstract There is an urgent need to improve conventional cancer‐treatments by preventing detrimental side effects, cancer recurrence and metastases. Recent studies have shown that presence of senescent cells in tissues treated with chemo‐ or radiotherapy can be used predict the effectiveness treatment. However, although accumulation one hallmarks cancer, surprisingly little progress has been made development strategies for their detection vivo. To address a lack tools, we developed...
Abstract Aberrantly accumulated metabolites elicit intra- and inter-cellular pro-oncogenic cascades, yet current measurement methods require sample perturbation/disruption lack spatio-temporal resolution, limiting our ability to fully characterize their function distribution. Here, we show that Raman spectroscopy (RS) can directly detect fumarate in living cells vivo animal tissues ex vivo, RS distinguish between Fumarate hydratase (Fh1)-deficient Fh1-proficient based on concentration....
Measurements of the conversion hyperpolarized [1-(13)C]pyruvate into lactate, in reaction catalyzed by lactate dehydrogenase, have shown promise as a metabolic marker for presence disease and response to treatment. However, it is unclear whether this represents net flux label from pyruvate or exchange isotope between metabolites that are close chemical equilibrium. Using saturation inversion transfer experiments, we show there significant murine lymphoma vivo. The rate constants estimated...
The primary aim of this study was to assess the potential in vivo photoacoustic tomography for direct functional measurement ovarian tumor response antiangiogenic therapy. <b>Methods:</b> In studies were performed with institutional animal care and use committee approval. We used an orthotopic mouse model cancer treated trebananib (<i>n</i> = 9) or vehicle 9). Tumor-bearing mice randomized into groups at day 10 dosed on days 12, 15, 18 after implantation. Photoacoustic blood draws then 24 h...
Abstract No clinically validated biomarkers exist to image tumor responses antiangiogenic therapy. Here, we report the utility of hyperpolarized 13C magnetic resonance spectroscopy (MRS) detect early effects anti-VEGF In two colorectal cancer xenograft models, displaying differential sensitivity VEGF blockade, compared MRS with measurements perfusion using dynamic contrast agent–enhanced (DCE)-MRI and cellularity diffusion-weighted MRI apparent diffusion coefficient (ADC) tissue water....
Optoacoustic tomography (OT) is now widely used in preclinical imaging; however, the precision (repeatability and reproducibility) of OT has yet to be determined. <b>Methods:</b> We a commercial small-animal system. Measurements stable phantoms were independently assess impact system variables on (using coefficient variation, COV), including acquisition wavelength, rotational position, frame averaging. Variables due animal handling physiology, such as anatomic placement anesthesia...