A5040536041- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Synthesis and biological activity
- Click Chemistry and Applications
- Computational Drug Discovery Methods
- Virology and Viral Diseases
- Bacillus and Francisella bacterial research
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- X-ray Diffraction in Crystallography
- Identification and Quantification in Food
- Crystallization and Solubility Studies
- Hepatitis B Virus Studies
- Viral Infections and Outbreaks Research
- Environmental DNA in Biodiversity Studies
- Viral Infections and Immunology Research
- Bioactive Compounds and Antitumor Agents
- Plant Virus Research Studies
- Metabolomics and Mass Spectrometry Studies
- Synthesis and Biological Evaluation
- Animal Virus Infections Studies
- Cancer therapeutics and mechanisms
- Fermentation and Sensory Analysis
- Synthesis of β-Lactam Compounds
- Plant and Fungal Interactions Research
Federal Medical-Biological Agency
2020-2023
Lomonosov Moscow State University
2023
Institute of Biochemistry and Genetics of Ufa Scientific Centre
2019
Moscow Institute of Physics and Technology
2016-2019
Central Research Institute of Epidemiology
2018-2019
Skolkovo Institute of Science and Technology
2018-2019
Many pharmaceutical companies are avoiding the development of novel antibacterials due to a range rational reasons and high risk failure. However, there is an urgent need for antibiotics especially against resistant bacterial strains. Available in silico models suffer from many drawbacks and, therefore, not applicable scoring molecules with structural diversity by their antibacterial potency. Considering this, overall aim this study was develop efficient model able find compounds which have...
Novel variously substituted thiohydantoin-based dispiro-indolinones were prepared using a regio- and diastereoselective synthetic route from 5-arylidene-2-thiohydantoins, isatines, sarcosine. The obtained molecules subsequently evaluated in vitro against the cancer cell lines LNCaP, PC3, HCTwt, HCT(-/-). Several compounds demonstrated relatively high cytotoxic activity vs. LNCaP cells (IC50 = 1.2-3.5 µM) reasonable selectivity index (SI 3-10). Confocal microscopy revealed that conjugate of...
In 2020, SARS-CoV-2 has spread rapidly across the globe, with most nations failing to prevent or substantially delay its introduction. While many countries have imposed some limitations on trans-border passenger traffic, effect of these measures global COVID-19 strains remains unclear. Here, we report an analysis 3206 whole-genome sequences samples from 78 regions Russia covering period before variants concern (between March and November 2020). We describe recurring imports multiple into...
Plants are widely used for food and beverage preparation, most often in the form of complex mixtures dried ground parts, such as teas, spices or herbal medicines. Quality control products is important due to potential health risks from presence unlabelled components absence claimed ones. A promising approach analyse DNA metabarcoding its high resolution sensitivity. However, this method's application analysis requires several methodology optimizations extraction, amplification library...
Advances in the next generation sequencing (NGS) technologies have significantly increased our ability to detect new viral pathogens and systematically determine spectrum of viruses prevalent various biological samples. In addition, this approach has also helped establishing associations viromes with many diseases. However, unlike metagenomic studies using 16S rRNA for detection bacteria, it is impossible create universal oligonucleotides target all known novel viruses, owing their genomic...
<title>Abstract</title> SARS-CoV-2 has spread rapidly across the globe, with most nations failing to prevent or substantially delay its introduction. While many countries have imposed some limitations on trans-border passenger traffic, effect of these measures COVID-19 strains remains unclear. Here, we report an analysis whole-genome sequencing 3206 samples from 78 regions Russia covering period between March and November 2020. We describe recurring imports multiple throughout this period,...
SARS-CoV-2 has spread rapidly across the globe, with most nations failing to prevent or substantially delay its introduction. While many countries have imposed some limitations on trans-border passenger traffic, effect of these measures COVID-19 strains remains unclear. Here, we report an analysis whole-genome sequencing 3206 samples from 78 regions Russia covering period between March and November 2020. We describe recurring imports multiple throughout this period, giving rise 457 uniquely...
Antibiotic resistance is a serious constraint to the development of new effective antibacterials. Therefore, discovery antibacterials remains one main challenges in modern medicinal chemistry. This study was undertaken identify novel molecules with antibacterial activity.Using our unique double-reporter system, in-house large-scale HTS campaign conducted for identification potency small-molecule compounds. The construction allows us visually assess underlying mechanism action. After initial...
Analysis of genomic variability pathogens associated with heightened public health concerns is an opportunity to track transmission routes the disease and helps develop more effective vaccines specific diagnostic tests. We present findings a detailed analysis SARS-CoV-2 Omicron variant that spread in Russia between 8 December 2021 30 January 2022. performed phylogenetic viral isolates collected Moscow (n = 589) downloaded from GISAID 397), identified BA.1 lineage was predominant during this...
Introduction: A variety of organic compounds has been reported to have antibacterial activity. However, antimicrobial resistance is one the main problems current anti-infective therapy, and development novel antibacterials challenges drug discovery. Methods: Using our previously developed dual-reporter High-Throughput Screening (HTS) platform, we identified a series furanocoumarins as having high The construction reporter system allows us differentiate three mechanisms action for active...
Objective of the study was development a method for detection and quantitative analysis (realtime RT-PCR) to identify genetic markers Lassa virus - LASV-Fl. Materials methods. We utilized all available in GenBank database ( https://www.ncbi.nlm.nih.gov/genbank/ ) sequences that have been aligned conservative sites applying BioEdit 7.2.5 software package (IbisBiosciences, USA). To test developed PCR kit, control panel RNA pseudo-viral particles, 27 viral strains belonging different families,...
The key issue in the development of novel antimicrobials is a rapid expansion new bacterial strains resistant to current antibiotics. Indeed, World Health Organization has reported that bacteria commonly causing infections hospitals and community, e.g. E. Coli, K. pneumoniae S. aureus, have high resistance vs last generations cephalosporins, carbapenems fluoroquinolones. During past decades, only few successful efforts develop launch antibacterial medications been performed. This study aims...
Recombination is one of the mechanisms SARS-CoV-2 evolution along with occurrence point mutations, insertions, and deletions. Recently, recombinant variants have been registered in different countries, some them become circulating forms. In this work, we performed screening genomic sequences to identify recombination events co-infections various strains virus detected Russia from February 2020 March 2022. The study included 9336 genomes COVID-19 pathogen obtained as a result high-throughput...
Next generation sequencing (NGS) technologies have greatly enhanced our ability to identify new viral pathogens in various types of biological samples. This approach has led the discovery viruses and revealed hidden associations viromes with many diseases. However, unlike 16S rRNA, which allows for bacterial detection by metabarcoding, diversity variability genomes render creation universal oligonucleotides targeting all known novel impossible. While wholegenome solves this problem, its...