A5022955273- T-cell and B-cell Immunology
- Contact Dermatitis and Allergies
- Immune Cell Function and Interaction
- Tattoo and Body Piercing Complications
- Body Image and Dysmorphia Studies
- Nanoparticles: synthesis and applications
- SARS-CoV-2 and COVID-19 Research
- Allergic Rhinitis and Sensitization
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Immune responses and vaccinations
- Drug-Induced Adverse Reactions
- Pesticide Residue Analysis and Safety
- Occupational exposure and asthma
- Agricultural safety and regulations
- Ruminant Nutrition and Digestive Physiology
- Animal health and immunology
- Dermatology and Skin Diseases
- Microplastics and Plastic Pollution
- Muscle metabolism and nutrition
- Additive Manufacturing and 3D Printing Technologies
- Pesticide Exposure and Toxicity
- Virology and Viral Diseases
- Mast cells and histamine
- Historical Medical Research and Treatments
Federal Institute for Risk Assessment
2020-2025
Ludwig-Maximilians-Universität München
2012-2024
German Rheumatism Research Centre
2020
University Medical Center Hamburg-Eppendorf
2016
Max Planck Institute of Biochemistry
2015-2016
Universität Hamburg
2016
Munich Cluster for Systems Neurology
2015-2016
University of Oxford
2016
John Radcliffe Hospital
2016
Center for Integrated Protein Science Munich
2015-2016
Psoriasis vulgaris is a common T cell–mediated inflammatory skin disease with suspected autoimmune pathogenesis. The human leukocyte antigen (HLA) class I allele, HLA-C*06:02, the main psoriasis risk gene. Epidermal CD8+ cells are essential for development. Functional implications of HLA-C*06:02 and mechanisms lesional cell activation in psoriasis, however, remained elusive. Here we identify melanocytes as skin-specific target an HLA-C*06:02–restricted psoriatic response. We found that...
In an in vitro nanotoxicity system, cell–nanoparticle (NP) interaction leads to the surface adsorption, uptake, and changes into nuclei/cell phenotype chemistry, as indicator of oxidative stress, genotoxicity, carcinogenicity. Different types nanomaterials their chemical composition or "corona" have been widely studied context with nanotoxicology. However, rare reports are available, which delineate details cell shape index (CSI) nuclear area factors (NAFs) a descriptor type nanomaterials....
The emergence of low-cost 3D printing (particularly with pens) in the home and school environment is a matter concern since there lack data confirming its safe application. filament washed water particle emission during (captured water) were analyzed. In this report, we provide important insights about toxicity, intracellular mitochondrial stress, cellular metabolic alterations as mechanistic response after exposure emissions released pen polylactic acid (PLA) PLA filaments additives (carbon...
To characterize phenotypes of T cells that accumulated in multiple sclerosis (MS) lesions, to compare the lesional T-cell receptor (TCR) repertoire subsets peripheral blood, and identify paired α β chains from single CD8(+) an index patient who we followed for 18 years.We combined immunohistochemistry, laser microdissection, single-cell multiplex PCR subtypes TCRα TCRβ individual brain-infiltrating frozen brain sections. The TCR repertoires were analyzed by pyrosequencing.We found a Vβ1(+)...
The severity of global pandemic due to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has engaged the researchers and clinicians find key features triggering viral infection lung cells. By utilizing such crucial information, scientists try combat spread virus. Here, in this work, we performed silico analysis protein-protein interactions between receptor-binding domain (RBD) spike protein human angiotensin-converting enzyme 2 (hACE2) receptor highlight alteration that happened...
Introduction Psoriasis is a T-cell mediated autoimmune skin disease. HLA-C*06:02 the main psoriasis-specific risk gene. Using Vα3S1/Vβ13S1 receptor (TCR) from lesional psoriatic CD8 + clone we had discovered that, as an underlying pathomechanism, mediates response against melanocytes in psoriasis, and identified epitope ADAMTS-like protein 5 (ADAMTSL5) melanocyte autoantigen. The conditions activating genetically predisposed individuals throughout life remain incompletely understood. Here,...
Abstract New Approach Methodologies (NAMs), broadly understood to include in silico, chemico, vitro and ex vivo methods, show great potential advancing risk assessment albeit their regulatory implementation is lagging. The EFSA Guidance on of nanomaterials (EFSA Nano‐RA) suggests nano‐specific best achieved through Integrated Approaches Testing Assessment (IATAs) with NAMs as the first choice generate new information. Integrating promises several advantages such a better human focus, more...
In polymyositis and inclusion body myositis, muscle fibers are surrounded invaded by CD8-positive cytotoxic T cells expressing the αβ-T cell receptor (αβ-TCR) for antigen. a rare variant of similarly attacked CD8-negative γδ-TCR (γδ-T cell-mediated myositis). We investigated antigen specificity human previously identified in an autoimmune tissue lesion γδ-T myositis. show that this Vγ1.3Vδ2-TCR, termed M88, recognizes various proteins from different species. Several these belong to...
Nickel is the most frequent cause of T cell-mediated allergic contact dermatitis worldwide. In vitro, CD4+ cells from all donors respond to nickel but involved αβ cell receptor (TCR) repertoire has not been comprehensively analyzed.We introduce CD154 (CD40L) upregulation as a fast, unbiased, and quantitative method detect nickel-specific ex vivo in blood clinically characterized non donors. Naïve (CCR7+ CD45RA+) memory (not naïve) CD154+ were analyzed by flow cytometry after 5 hours...
Abstract Plenty of new approach methodologies (NAMs) for risk assessment have been developed but only some are included in OECD Test Guidelines (TGs) regulatory implementation. Nevertheless, NAMs increasingly applied, e.g. nanomaterial (NM) assessments. The EFSA Guidance on NM suggests that NAM‐derived data concerning degradation/dissolution (in relevant biofluids), intestinal uptake/crossing, genotoxicity, cytotoxicity, oxidative stress, (pro‐)inflammatory potential and barrier integrity,...
Dendritic cells (DC) play a central role in the pathogenesis of allergic contact dermatitis (ACD), most prevalent form immunotoxicity humans. However, knowledge on allergy-induced DC maturation is still limited and proteomic studies, allowing to unravel molecular effects allergens, remain scarce. Therefore, we conducted global analysis human monocyte-derived dendritic (MoDC) treated with NiSO 4 , prominent cause ACD compared alterations induced by bacterial trigger lipopolysaccharide (LPS)....
Abstract Background Apart from Ni 2+ , Co and Pd ions commonly trigger T cell‐mediated allergic contact dermatitis. However, in vitro frequencies of metal‐specific cells the mechanisms antigen recognition remain unclear. Methods Here, we utilized a CD154 upregulation assay to quantify ‐, ‐specific CD4+ peripheral blood mononuclear (PBMC). Involved αβ cell receptor (TCR) repertoires were analyzed by high‐throughput sequencing. Results Peripheral incubation with NiSO 4 CoCl 2 PdCl increased +...
Allergic contact dermatitis is a widespread T cell-mediated inflammatory skin disease, but
Abstract Background Contrary to Ni 2+ ‐ and Co ‐induced allergic contact dermatitis (ACD), reactions against Pd are rare. However, activates a larger T cell fraction in vitro , suggesting an inefficient skin penetration. Objectives This study compares penetration from commonly used diagnostic patch test preparations (PTPs) aqueous metal salt solutions. Methods Using Franz diffusion assays, we applied the metals PTPs (5% NiSO 4 1% CoCl 2 2% PdCl 3% Na ) solution pigskin for 48 h, thereby...
Resident memory T lymphocytes (TRM ) of epithelial tissues and the Bm protect their host tissue. To what extent these cells are mobilized contribute to systemic immune reactions is less clear. Here, we show that in secondary measles-mumps-rubella (MMR) vaccine, CD4+ TRM into blood within 16 48 h after immunization humans. This mobilization cognate: recognizing other antigens not mobilized, unless they cross-react with vaccine. We also demonstrate through methylome analyses from Bm. These...
To identify target antigens presented by human leukocyte antigen (HLA)-A*02:01 to the myelin-reactive T-cell receptor (TCR) 2D1, which was originally isolated from a CD8+ clone recognizing proteolipid protein (PLP) in context of HLA-A*03:01, we employed new search technology.We used our recently developed technology that employs plasmid-encoded combinatorial peptide libraries and highly sensitive single cell detection system endogenous candidate peptides mice origin. We validated independent...