A5101716630- Heat shock proteins research
- SARS-CoV-2 and COVID-19 Research
- Endoplasmic Reticulum Stress and Disease
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Ubiquitin and proteasome pathways
- NF-κB Signaling Pathways
- Exercise and Physiological Responses
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- interferon and immune responses
- Protein Degradation and Inhibitors
- Adipose Tissue and Metabolism
- COVID-19 Clinical Research Studies
- Viral Infections and Immunology Research
- Viral Infections and Vectors
- Viral Infectious Diseases and Gene Expression in Insects
- Nuclear Structure and Function
- RNA Research and Splicing
- Animal Disease Management and Epidemiology
- Heme Oxygenase-1 and Carbon Monoxide
- thermodynamics and calorimetric analyses
- Protein purification and stability
- Herpesvirus Infections and Treatments
- Influenza Virus Research Studies
Istituto di Farmacologia Traslazionale
2013-2024
National Research Council
2014-2018
Institute of Neurobiology and Molecular Medicine
2001-2010
Istituto di Genetica Molecolare
2007
Medtronic (Ireland)
2003
University of Rome Tor Vergata
1994-2000
Czech Academy of Sciences, Institute of Experimental Medicine
2000
University of L'Aquila
1996-1997
Sapienza University of Rome
1996
University of Ferrara
1986
Prostaglandins (PGs) function as intracellular signal mediators in the regulation of a variety physiological and pathological processes, including inflammation immune responses. Cyclopentenone PGs are characterized by antiviral activity against several viruses, human immunodeficiency virus type 1 (HIV-1), ability to induce heat shock protein expression through activation transcription factor. Here we report that PGA1 is potent inhibitor nuclear factor-kappa B (NF-kappa B) cells NF-kappa...
In heat-shocked human cells, heat shock factor 1 activates transcription of tandem arrays repetitive Satellite III (SatIII) DNA in pericentromeric heterochromatin. RNAs remain associated with sites nuclear stress bodies (nSBs). Here we use real-time RT-PCR to study the expression these genomic regions. Transcription is highly asymmetrical and most transcripts contain G-rich strand repeat. A low level detectable unstressed cells a 10(4)-fold induction occurs after shock. are induced by wide...
SARS-CoV-2, the causative agent of COVID-19, has caused an unprecedented global health crisis. The SARS-CoV-2 spike, a surface-anchored trimeric class-I fusion glycoprotein essential for viral entry, represents key target developing vaccines and therapeutics capable blocking virus invasion. emergence spike variants that facilitate spread may affect vaccine efficacy highlights need to identify novel antiviral strategies COVID-19 therapy. Here, we demonstrate nitazoxanide, antiprotozoal with...
Herpes simplex viruses (HSV) are ubiquitous pathogens causing a variety of diseases ranging from mild illness to severe life-threatening infections. HSV utilize cellular signaling pathways and transcription factors promote their replication. Here we report that type 1 (HSV-1) induces persistent activation factor NF-κB, critical regulator genes involved in inflammation, by activating the IκB kinase (IKK) early phase infection. Activated NF-κB enhances HSV-1 gene expression. HSV-1-induced is...
Abstract Carcinoma of the uterine cervix is one highest causes mortality in female cancer patients worldwide, and improved treatment options for this type malignancy are highly needed. Local hyperthermia has been successfully used combination with systemic administration cisplatin-based chemotherapy phase I/II clinical studies. Heat-induced expression cytoprotective antiapoptotic heat shock proteins (HSP) a known complication hyperthermia, resulting thermotolerance chemoresistance hindering...
The global health emergency for SARS-CoV-2 (COVID-19) created an urgent need to develop new treatments and therapeutic drugs. In this study, we tested, the first time on human cells, a tetravalent neutralizing antibody (15033-7) targeting Spike protein synthetic peptide homologous dipeptidyl peptidase-4 (DPP4) receptor host cells. Both could represent powerful immunotherapeutic candidates COVID-19 treatment. infection begins in proximal airways, namely alveolar type 2 (AT2) cells of distal...
The heat-shock response, a fundamental defense mechanism against proteotoxic stress, is regulated by family of transcription factors (HSF). In humans HSF1 considered the central regulator heat-induced transcriptional responses. main targets for are specific promoter elements (HSE) located upstream genes encoding cytoprotective proteins (HSP) with chaperone function. addition to its function, was recently hypothesized play more complex role, regulating expression non-HSP genes; however,...
The nucleolus, a dynamic nuclear compartment long regarded as the cell ribosome factory, is emerging an important player in regulation of survival and recovery from stress. In larger eukaryotes, stress-induced transcriptional response mediated by family heat-shock transcription factors. Among these, HSF1, considered master regulator responses, controls expression cytoprotective heat shock proteins (HSPs), molecular chaperones/cochaperones constituting major component protein quality control...
Heat shock proteins (HSPs) have a cytoprotective role in several human diseases, including ischemia and viral infection. Nuclear factor-κB (NF-κB) is critical regulator of inflammation virus replication. Here we report that class serine protease inhibitors with NF-κB-inhibitory activity are potent HSP inducers via activation heat transcription factor 1 (HSF1) cells. 3,4-Dichloroisocoumarin, the most effective compound, rapidly induces HSF1 DNA binding phosphorylation, leading to translation...
The zinc finger AN1-type domain 2a gene, also known as arsenite-inducible RNA-associated protein (AIRAP), was recently identified a novel human canonical heat shock gene strictly controlled by factor (HSF) 1. Little is about AIRAP regulation in cells. Here we report that bortezomib, proteasome inhibitor with anticancer and antiangiogenic properties used the clinic for treatment of multiple myeloma, potent inducer expression Using endothelial cells model, unraveled molecular mechanism...
Cyclopentenone prostaglandins are potent inhibitors of virus replication. The antiviral activity has been associated with the induction 70-kDa heat shock protein (HSP70) synthesis. In this report, we describe that in African green monkey kidney cells infected Sendai (SV) and treated prostaglandin A1 (PGA1), SV synthesis was selectively blocked as long HSP70 being synthesized by host cell. block appeared to be at translational level, indicated following (i) PGA1 had no effect on primary...
Heat shock factor-1 (HSF1) is the central regulator of heat-induced transcriptional responses leading to rapid expression molecular chaperones that protect mammalian cells against proteotoxic stress. The main targets for HSF1 are specific promoter elements (HSE) located upstream heat genes encoding a variety proteins, including HSP70, HSP90, HSP27, and other proteins network. Herein we report zinc finger AN1-type domain-2a gene, also known as AIRAP, behaves canonical whose...
Prostaglandins of the A type (PGA) exert a cytoprotective activity during hyperthermia and virus infection. This effect is associated with induction heat shock proteins (HSP) in mammalian cells. We now report that, human monocytes, PGA 1 able to induce synthesis iron‐binding, redox‐regulated protein ferritin. L‐chain ferritin consequent substantial increase accumulation transcripts ‐treated cells, whereas H‐chain regulated post‐transcriptionally, consequently reduction iron‐regulatory...
Prostaglandins of the A type (PGA) induce synthesis 70 kDa heat shock proteins (hsp70) in a large variety mammalian cells. Induction hsp70 has been associated with cytoprotective effect PGA1 after virus infection or thermal injury. In present report we provide evidence that, murine myoblasts, is not able to expression, whereas it increases constitutive protein, hsc70, and dramatically induces 32 protein (p32). The p32 identified as haem oxygenase. acts at transcriptional level by inducing...
Coronaviridae is recognized as one of the most rapidly evolving virus family a consequence high genomic nucleotide substitution rates and recombination. The comprises large number enveloped, positive-sense single-stranded RNA viruses, causing an array diseases varying severity in animals humans. To date, seven human coronaviruses (HCoV) have been identified, namely HCoV-229E, HCoV-NL63, HCoV-OC43 HCoV-HKU1, which are globally circulating population (seasonal HCoV, sHCoV), highly pathogenic...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in a global pandemic causing over 195 million infections and more than 4 fatalities as of July 2021.To date, it been demonstrated that number mutations the spike glycoprotein (S protein) SARS-CoV-2 variants concern abrogate or reduce neutralization potency several therapeutic antibodies vaccine-elicited antibodies. Therefore, development additional vaccine platforms with improved supply logistic profile remains...