A5036135292- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related Molecular Pathways
- MicroRNA in disease regulation
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Nuclear Receptors and Signaling
- Alzheimer's disease research and treatments
- Cervical Cancer and HPV Research
- Bioinformatics and Genomic Networks
- Cancer Cells and Metastasis
- Endoplasmic Reticulum Stress and Disease
- Spaceflight effects on biology
- RNA Research and Splicing
- Pain Mechanisms and Treatments
- 3D Printing in Biomedical Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Amyotrophic Lateral Sclerosis Research
- Nuclear Structure and Function
- Neuroscience and Neuropharmacology Research
- COVID-19 Clinical Research Studies
- Axon Guidance and Neuronal Signaling
European Brain Research Institute
2015-2025
Ministry of Defence
2024-2025
University of Missouri
2021
Istituto di Farmacologia Traslazionale
2014
Italian Institute of Technology
2011
Deutsches Historisches Institut Rom
2007-2008
MicroRNAs (miRNAs) regulate gene expression at post-transcriptional level and are key modulators of immune system, whose dysfunction contributes to the progression neuroinflammatory diseaseas such as amyotrophic lateral sclerosis (ALS), most widespread motor neuron disorder. ALS is a non-cell-autonomous disease targeting neurons neighboring glia, with microgliosis directly contributing neurodegeneration. As limited information exists on miRNAs dysregulations in ALS, we examined this topic...
The identification of early and stage-specific biomarkers for Alzheimer's disease (AD) is critical, as the development disease-modification therapies may depend on discovery validation such markers. reliable depends new diagnostic algorithms to computationally exploit information in large biological datasets. To identify potential from mRNA expression profile data, we used Logic Mining method unbiased analysis a microarray dataset anti-NGF AD11 transgenic mouse model. gene brain regions was...
The human intestinal Caco-2 cell line has been extensively used as a model of the barrier. However, it is widely reported in literature that culture-related conditions, well different lines utilized laboratories, often lead to problems reproducibility making difficult compare results. We developed new cell-maintenance protocol which cells were subcultured at 50% confluence instead 80% confluence, usually suggested. Using this protocol, retained higher proliferation potential resulting...
Increasing evidence points to a key role played by epithelial-mesenchymal transition (EMT) in cancer progression and drug resistance. In this study, we used wet silico approaches investigate whether EMT phenotypes are associated resistance target therapy non-small cell lung model system harboring activating mutations of the epidermal growth factor receptor. The combination different analysis techniques allowed us describe intermediate/hybrid complete respectively HCC827- HCC4006-derived...
In neurons, specific mRNAs are transported in a translationally repressed manner along dendrites or axons by transport ribonucleic-protein complexes called RNA granules. ZBP1 is one binding protein present RNPs, where it transports and represses the translation of cotransported mRNAs, including β-actin mRNA. The release mRNA from its subsequent depends on phosphorylation Src kinase, but little known about how this process regulated. Here we demonstrate that ribosomal-associated RACK1,...
Background Neuroblastoma (NB) is one of the most aggressive tumors that occur in childhood. Although genes, such as MYCN, have been shown to be involved aggressiveness disease, identification new biological markers still desirable. The induction differentiation strategies used treatment neuroblastoma. A-type lamins are components nuclear lamina and differentiation. We studied role Lamin A/C progression Methodology/Principal Findings Knock-down (LMNA-KD) neuroblastoma cells blocked retinoic...
// Martina Antonelli 1, 2 , Flavie Strappazzon 1 Ivan Arisi 3 Rossella Brandi Mara D'Onofrio Manolo Sambucci 4 Gwenola Manic Ilio Vitale 2, 5 Daniela Barilà Venturina Stagni Instituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Fondazione Santa Lucia, Rome, Italy Department of Biology, University Rome 'Tor Vergata', Genomics Facility, European Brain Research Institute (EBRI) 'Rita Levi-Montalcini', Neuroimmunology Unit, IRCCS, Regina Elena National Cancer Center Institute,...
Restoration of wild-type p53 tumor suppressor function has emerged as an attractive anticancer strategy. Therapeutics targeting the two p53-negative regulators, MDM2 and MDM4, have been developed, but most agents selectively target ability only one these molecules to interact with p53, leaving other free operate. Therefore, we developed a method that targets activity MDM4 simultaneously based on recent studies indicating formation MDM2/MDM4 heterodimer complexes are required for efficient...
Abstract A decrease in brain-derived neurotrophic factor (BDNF), a neurotrophin essential for synaptic function, plasticity and neuronal survival, is evident early the progression of Alzheimer’s disease (AD), being apparent subjects with mild cognitive impairment or AD, both proBDNF mature BDNF levels are positively correlated measures. delivery is, therefore, considered great interest as potentially useful therapeutic strategy to contrast AD. Invasive administration has indeed been recently...
GABA, the main inhibitory transmitter in adulthood, early postnatal development exerts a depolarizing and excitatory action. This effect, which results from high intracellular chloride concentration ([Cl − ] i ), promotes neuronal growth synaptogenesis. During second week, developmental regulated expression of cation-chloride cotransporter KCC2 accounts for shift GABA to hyperpolarizing direction. Changes homeostasis associated with [Cl have been found several neurological disorders,...
Nerve growth factor (NGF) was discovered because of its neurotrophic actions on sympathetic and sensory neurons in the developing chicken embryo. NGF subsequently found to influence regulate function many neuronal non cells adult organisms. Little is known, however, about possible during early embryonic stages. However, mRNAs encoding for receptors TrkA p75(NTR) are expressed at very stages avian embryo development, before nervous system formed. The question, therefore, arises as what might...
Adult neurogenesis is a multistep process regulated by several extrinsic factors, including neurotrophins. Among them, little known about the role of nerve growth factor (NGF) in neurogenic niches mouse. Here we analyzed biology adult neural stem cells (NSCs) from subventricular zone (SVZ) AD11 anti-NGF transgenic mice, which expression recombinant antibody aD11 leads to chronic postnatal neutralization endogenous NGF. We showed that AD11-NSCs proliferate 10-fold less, with respect their...
Since the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), need for an effective vaccine has appeared crucial stimulating immune system responses to produce humoral/cellular immunity and activate immunological memory. It been demonstrated that SARS-CoV-2 variants escape neutralizing elicited by previous infection and/or vaccination, leading new waves cases reinfection. The study aims gain into reinfections, particularly infections vaccination-induced protection. We...
Abstract Lamin A/C is a nuclear type V intermediate filament protein part of the meshwork structure underlying inner membrane (nuclear lamina), which plays numerous roles, including maintenance shape, heterochromatin organization, and transcriptional regulation. Our group has demonstrated role in different pathophysiological conditions. Here, we investigated for first time how affects neuronal maturation rat cerebellar granule cells (GCs). Primary GCs where silenced Lmna gene constituted our...
Heat shock factor-1 (HSF1) is the central regulator of heat-induced transcriptional responses leading to rapid expression molecular chaperones that protect mammalian cells against proteotoxic stress. The main targets for HSF1 are specific promoter elements (HSE) located upstream heat genes encoding a variety proteins, including HSP70, HSP90, HSP27, and other proteins network. Herein we report zinc finger AN1-type domain-2a gene, also known as AIRAP, behaves canonical whose...
OBJECTIVE Haptoglobin (Hp) is upregulated in both inflammation and obesity. The low chronic inflammatory state, caused by massive adipose tissue macrophage (ATM) infiltration found obesity, adiponectin have been implicated the development of insulin resistance hepatosteatosis. aim this work was to investigate whether how Hp interferes with onset obesity-associated complications. RESEARCH DESIGN AND METHODS Hp-null (Hp−/−) wild-type (WT) mice were metabolically profiled under chow-food diet...
Nerve growth factor (NGF) is critical for neuronal physiology during development and adulthood. Despite the well-recognized effect of NGF on neurons, less known about whether can actually affect other cell types in central nervous system (CNS). In this work, we show that astrocytes are susceptible to changes ambient levels NGF. First, observe interfering with signaling vivo via constitutive expression an antiNGF antibody induces astrocytic atrophy. A similar asthenic phenotype encountered...