A5026408372- Chronic Kidney Disease and Diabetes
- Diabetes Treatment and Management
- Diet and metabolism studies
- Pancreatic function and diabetes
- Neuropeptides and Animal Physiology
- Phenothiazines and Benzothiazines Synthesis and Activities
- Color perception and design
- Chemical Synthesis and Analysis
- Nitric Oxide and Endothelin Effects
- Adipokines, Inflammation, and Metabolic Diseases
- Synthesis and Biological Evaluation
- Diabetes Management and Research
- Peptidase Inhibition and Analysis
- Advanced Glycation End Products research
- Diabetes and associated disorders
- Renal Diseases and Glomerulopathies
- DNA Repair Mechanisms
- Nutrition and Health in Aging
- Liver Disease Diagnosis and Treatment
- Barrier Structure and Function Studies
- Dialysis and Renal Disease Management
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Receptor Mechanisms and Signaling
- Diet, Metabolism, and Disease
Akita University
2015-2024
Sapporo Medical University
2004-2024
Tohoku University
2015-2024
Tokushima Bunri University
2023
Kochi Medical School Hospital
2020
Showa Pharmaceutical University
2020
Nihon University
1989-2019
Musashino Art University
2017
University of Toyama
2009-2017
Vanderbilt University Medical Center
2009-2012
Ca(2+) is absorbed across intestinal epithelial monolayers via transcellular and paracellular pathways, an active form of vitamin D(3), 1alpha,25-dihydroxyvitamin D(3) [1alpha,25(OH)(2)D(3)], known to promote absorption. However, the molecules driving absorption its D dependency remain obscure. Because tight junction proteins claudins are suggested channels for selective ions between neighboring cells, we hypothesized that specific might facilitate transport expression these could be induced...
Abstract Aims/Introduction To investigate the prevalence of sarcopenia, its related factors and indicators physical evaluation in elderly diabetes patients. Materials Methods This was a cross‐sectional observation study. A total 267 patients (159 men, 108 women) aged >65 years were recruited present Skeletal muscle mass index, grip strength usual gait speed measured to diagnose sarcopenia according Asian Working Group for Sarcopenia. Body composition using bioelectrical impedance...
Differential susceptibility to diabetic nephropathy has been observed in humans, but it not well defined inbred strains of mice. The present studies characterized the severity six mouse including C57BL/6J, DBA/2J, FVB/NJ, MRL/MpJ, A/J, and KK/HlJ Diabetes mellitus was induced using low-dose streptozotocin injection. Progression renal injury evaluated by serial measurements urinary albumin excretion, glomerular filtration rate (GFR), terminal assessment morphology over 25 weeks. Despite...
Gap-junction plaques are often observed with tight-junction strands of vascular endothelial cells but the molecular interaction and functional relationships between these two junctions remain obscure. We herein show that gap-junction proteins connexin40 (Cx40) Cx43 colocalized coprecipitated molecules occludin, claudin-5, ZO-1 in porcine blood-brain barrier (BBB) cells. Gap junction blockers 18beta-glycyrrhetinic acid (18beta-GA) oleamide (OA) did not influence expression Cx40, Cx43,...
Among tight-junction proteins, claudins, which play a key role in paracellular transport across epithelia, claudins 1 to 5 are expressed the intestine, and changes their abundance and/or distribution considered contribute various gastrointestinal diseases. We investigated, by reverse transcription-PCR, immunoblot, immunofluorescence analyses, other claudin species were mouse whether they showed unique expression profiles. Rabbit polyclonal antibodies against claudin-8, claudin-12, claudin-15...
Superoxide excess plays a central role in tissue damage that results from diabetes, but the mechanisms of superoxide overproduction diabetic nephropathy (DN) are incompletely understood. In present study, we investigated enzyme dismutase (SOD), major defender against superoxide, kidneys during development murine DN. We assessed SOD activity and expression isoforms two mouse models (C57BL/6-Akita KK/Ta-Akita) exhibit comparable levels hyperglycemia different susceptibility to observed...
Abstract The regulation of aberrant glucose metabolism in diabetes associated-kidney fibrosis is not well known. In this study we found the suppression SIRT3 protein level diabetic kidney, displays responsibility fibrogenic programming associated with glycolysis and such abnormal therapeutic target fibrosis. When analyzing different strains streptozotocin-induced mice model (fibrotic model: CD-1, less fibrotic C57Bl6), was kidney CD-1; further by systemic administration siRNA mice, showed...
Dipeptidyl peptidase-4 (DPP-4) inhibitor is a new class of anti-diabetic drug which exerts its glucose-lowering action by suppressing the degradation gut incretin hormone glucagon-like peptide-1 (GLP-1). To elucidate whether treatment with stronger DPP-4 on top angiotensin II type 1 receptor blocker (ARB) provides greater renal protective effects, we performed crossover study two inhibitors, sitagliptin and alogliptin, in twelve 2 diabetic patients incipient nephropathy taking ARBs. This...
OBJECTIVE—Increased urinary excretions of several plasma proteins with different molecular radii <55 Å and isoelectric points (pI), such as IgG, ceruloplasmin, transferrin, orosomucoid, have been independently reported to precede the development microalbuminuria in diabetic patients. We examined whether increases these would be parallel same patient. RESEARCH DESIGN AND METHODS—Urinary excretion rates mentioned above timed overnight urine samples were evaluated 61 normoalbuminuric...
To compare the effects of miglitol [an alpha‐glucosidase inhibitor (AGI) absorbed in intestine] and voglibose (an AGI not absorbed) on plasma glucagon‐like peptide‐1 (GLP‐1) gastric inhibitory polypeptide (GIP) levels, 26 24 Japanese type 2 diabetic patients were randomly assigned to receive or voglibose, respectively. After 12‐week administration both drugs, during 2‐h meal tolerance test, glucose, serum insulin total GIP significantly decreased active GLP‐1 was increased. Miglitol group...
Mitotic arrest deficient 2-like protein 2 (MAD2L2), also termed MAD2B or REV7, is involved in multiple cellular functions including translesion DNA synthesis (TLS), signal transduction, transcription, and mitotic events. MAD2L2 interacts with chromosome alignment-maintaining phosphoprotein (CAMP), a kinetochore-microtubule attachment cells, presumably through novel "WK" motif CAMP. Structures of complex binding regions the TLS proteins REV3 REV1 have revealed that has two faces for...
There is a close relationship between the gut microbiota and metabolic disorders. In this study, acute administration of glucagon-like peptide-1 receptor agonist (GLP-1RA) liraglutide to mice increased cecal levels caseinolytic protease B, component Escherichia coli, norepinephrine. Chemical sympathectomy blocked these events. Norepinephrine was found pass into intestinal lumen in vitro. c-Fos staining intermediolateral nucleus identified as indirect evidence sympathetic nervous system...
Adiponectin is an adipose-derived protein which has anti-inflammatory and anti-atherogenic properties in addition to insulin-sensitizing effects. To date, the role of adiponectin pathogenesis diabetic nephropathy remains unclear. The aim present study was explore relationship between renal tubular injury nephropathy. We determined serum urinary levels type 2 patients with normoalbuminuria (n = 19), microalbuminuria 18), overt 16), then analyzed correlations adiponectin,...
Decreased circulating levels of adiponectin, a novel adipose-derived adipocytokine, in obesity possibly contribute to the development insulin resistance which is major factor pathogenesis type 2 diabetes. The present study was conducted examine whether and adipose tissue adiponectin are modulated by chronic treatment with metformin intensive murine models diabetes, db/db mice C57BL/KsJ genetic background. Nine-week-old male were treated metformin, insulin, vehicle for 4 weeks. Expectedly,...
Renal superoxide excess, which is induced by an imbalance of the superoxide-producing enzyme NAD(P)H oxidase and superoxide-scavenging dismutase (SOD) under hyperglycemia, increases oxidative stress contributes to development diabetic nephropathy. In this study, we treated non-obese hypoinsulinemic C57BL/6-Ins2(Akita) (C57BL/6-Akita) mice with telmisartan (5 mg kg(-1) per day), angiotensin II type 1 receptor blocker, or amlodipine a calcium channel for 4 weeks compared effects these two...
Renal scintigraphy using 99mTc-mercaptoacetyltriglycine (99mTc-MAG3) is widely used for the assessment of renal function in humans. However, application this method to animal models disease currently limited, especially rodents. Here, we have applied 99mTc-MAG3 a mouse model unilateral ureteral obstruction (UUO) and evaluated its utility studying obstructive disease.UUO mice were generated by complete ligation left ureter. Sham-operated as control. was investigated on days 0, 1, 3, 6...
Abstract Background Intramuscular adipose tissue (IMAT) formation derived from muscle fibro‐adipogenic progenitors (FAPs) has been recognized as a pathological feature of sarcopenia. This study aimed to explore whether genetic and pharmacological gastric inhibitory polypeptide (GIP) receptor antagonism suppresses IMAT accumulation ameliorates sarcopenia in mice. Methods Whole body composition, grip strength, skeletal weight, tibialis anterior (TA) fibre cross‐sectional area (CSA) TA were...