A5042724286- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Hematopoietic Stem Cell Transplantation
- Histone Deacetylase Inhibitors Research
- Acute Lymphoblastic Leukemia research
- Advanced biosensing and bioanalysis techniques
- Hematological disorders and diagnostics
- Telomeres, Telomerase, and Senescence
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hemoglobinopathies and Related Disorders
- Sarcoma Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Genomics and Chromatin Dynamics
Instituto Nacional do Câncer
2007-2024
We studied the methylation status of p15INK4B and p16INK4A genes in 47 pediatric patients with primary MDS, its correlation subtype, role evolution MDS toward AML. Aberrant gene was detected 15 (32%), whereas only four demonstrated (8%). The frequency significantly higher RAEB RAEB-t subtypes (p < 0.003). also more frequent that characterize advanced stages disease 0.05). Evolution verified 17 (36%) patients. association clearly significant 0.008 p 0.05, respectively). These results suggest...
ABCB1 gene methylation analyses method to quantify status at specific sites of DNA.Here, we established HRM parameters evaluate the promoter gene.Our study is first standardize dissociation curve methylation.The association between and expression in cancer cell lines shows that this accurate reliable.
The aim of this study was to analyse the expression profiles DNMT1, DNMT3A, DNMT3B (components DNA methylation machinery), TET2 and APOBEC3B demethylation machinery) in pediatric MDS patients investigate their associations with subtypes, cytogenetics, evolution acute myeloid leukemia (AML) p15INK4B level.The expressions DNMT3B, TET2, were evaluated 39 by real-time quantitative PCR (qPCR). quantification levels (MtL) performed 20 pyrosequencing. Mann-Whitney test used evaluate possible...
Background and objective: Pediatric myelodysplastic syndrome (pMDS) is a group of rare clonal neoplasms with difficult diagnosis risk progression to acute myeloid leukemia (AML). The early stratification in groups essential choosing the treatment indication for allogeneic hematopoietic stem cell transplantation (HSCT). According Revised International Prognostic Scoring System, cytogenetic analysis has demonstrated an role prognosis. In pMDS, abnormal karyotypes are present 30-50% cases....
Background: Myelodysplastic syndrome (MDS) comprises a heterogeneous group of clonal hematopoietic stem cell diseases, characterized by dysplasias and apoptosis in bone marrow (BM) cytopenias peripheral blood. In this study, we analyzed MDS to verify associations with patient age, cellularity karyotypes investigate the role pathogenesis. Methods: Bone cells were collected from 81 patients primary MDS, which 60 adults 21 children. BM also 10 healthy donors for transplants, 5 children, as...