A5008833439- Hepatitis C virus research
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Hepatitis B Virus Studies
- HIV Research and Treatment
- Liver Disease Diagnosis and Treatment
- Plasma Diagnostics and Applications
- Laser-induced spectroscopy and plasma
- Barrier Structure and Function Studies
- Plasma Applications and Diagnostics
- RNA regulation and disease
- Virology and Viral Diseases
- Viral Infections and Immunology Research
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Aerosol Filtration and Electrostatic Precipitation
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
- Laser Material Processing Techniques
- Diamond and Carbon-based Materials Research
- Animal Virus Infections Studies
- Innovation and Socioeconomic Development
- interferon and immune responses
- Virus-based gene therapy research
Albert Einstein College of Medicine
2021-2025
Xi'an Institute of Optics and Precision Mechanics
2023-2024
University of Chinese Academy of Sciences
2023-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2023
Shandong Provincial QianFoShan Hospital
2023
Shandong First Medical University
2023
Taipei Veterans General Hospital
2008-2009
National Yang Ming Chiao Tung University
2008
Chikungunya virus (CHIKV) is an arthritogenic alphavirus that has re-emerged to cause large outbreaks of human infections worldwide. There are currently no approved antivirals for treatment CHIKV infection. Recently, we reported the ribonucleoside analog 4'-fluorouridine (4'-FlU) a highly potent inhibitor replication, and targets viral nsP4 RNA dependent polymerase. In mouse models, oral therapy with 4'-FlU diminished tissue burdens virus-induced disease signs. To provide critical evidence...
Abstract Viruses evolve multiple ways to interfere with NF-κB signaling, a key regulator of innate and adaptive immunity. Enterovirus 71 (EV71) is one primary pathogens that cause hand-foot-mouth disease. Here, we identify RelA(p65) as novel binding partner for EV71 2C protein from yeast two-hybrid screen. By interaction IPT domain p65, reduces the formation heterodimer p65/p50, predominant form NF-κB. We also show picornavirus family proteins inhibit activation associate p65 IKKβ. Our...
ABSTRACT Chikungunya virus (CHIKV) is an enveloped, positive-sense RNA that has re-emerged to cause millions of human infections worldwide. In humans, acute CHIKV infection causes fever and severe muscle joint pain. Chronic debilitating arthritis pain can persist for months years. To date, there are no approved antivirals against CHIKV. Recently, the ribonucleoside analog 4′-fluorouridine (4′-FlU) was reported as a highly potent orally available inhibitor SARS-CoV-2, respiratory syncytial...
ABSTRACT Phosphatidylinositol 4-phosphate (PI4P) is well known to be upregulated during hepatitis C virus (HCV) replication. The role of PI4 kinases in HCV has been extensively investigated. Whether the PI4P phosphatase Sac1 altered by remains unclear. Here, we identified ARFGAP1 a novel host factor for We further show that interacts with and inhibits elevation induced NS5A abrogated when coatomer protein I (COPI) pathway inhibited. also found an interaction between ARFGAP1. Furthermore,...
Chikungunya virus (CHIKV) is a re-emerging mosquito-transmitted RNA causing joint and muscle pain. To better understand how CHIKV rewires the host cell usurps functions, we generated systematic CHIKV-human protein-protein interaction map revealed several novel connections that will inform further mechanistic studies. One of these interactions, between viral protein E1 STIP1 homology U-box containing 1 (STUB1), was found to mediate ubiquitination degrade through proteasome. Capsid associated...
ABSTRACT Rubella virus (RuV) is an enveloped plus-sense RNA and a member of the Rubivirus genus. RuV infection in pregnant women can lead to miscarriage or array severe birth defects known as congenital rubella syndrome. Novel rubiviruses were recently discovered various mammals, highlighting spillover potential other humans. Many features rubivirus cycle remain unexplored. To promote study biology, here, we generated replication-competent recombinant VSV-RuV (rVSV-RuV) encoding...
Summary Aspirin has previously been reported to inhibit hepatitis C virus ( HCV ) replication. The aim of this study was investigate whether aspirin is involved in blocking entry. We found that inhibits the entry pp and infectious . level claudin‐1, an receptor, reduced by aspirin. Our results extend anti‐ effect step further reinforce role
ABSTRACT Claudin-1 is a hepatitis C virus (HCV) coreceptor required for viral entry. Although extensive studies have focused on claudin-1 as an anti-HCV target, little known about how the level of at cell surface regulated by host vesicular transport. Here, we identified interaction between and Sec24C, cargo-sorting component coat protein complex II (COPII) transport system. By interacting with Sec24C through its C-terminal YV, transported from endoplasmic reticulum (ER) eventually targeted...
Somatic mutations in the mitochondrial DNA (mtDNA) displacement loop (D-loop) region have been frequently detected various human cancers. In a previous study, we identified polyplasmic 260-bp tandem duplication and triplication mutation mtDNA D-loop of one gastric cancer. present adopted more sensitive back-to-back polymerase chain reaction method to screen for this duplication/triplication 197 cancers their adjacent non-cancerous tissues. Nine samples primary cancer (4.6%) were found harbor...
A two-dimensional axisymmetric fluid model is used to simulate the dynamics behavior of an atmospheric-pressure helium plasma jet in presence a parallel magnetic field. The generated coaxial dielectric barrier discharge (DBD) driven by pulsed direct-current voltage. Comparative analysis with and without field indicates that slightly thinner sheath inside tube present as result decreased accumulated electrons on inner surface tube. After streamer propagates outside tube, little more...
Improper disposal of waste oils containing hazardous components damages the environment and ecosystem, posing a significant threat to human life health. Here, we present method discharge-assisted laser-induced breakdown spectroscopy combined with filter paper sampling (DA-LIBS-FPS) detect trace source polluting elements. DA-LIBS-FPS significantly enhances spectral intensity by 1–2 orders magnitude due discharge energy deposition into plasma highly efficient laser-sample interaction on paper,...
Hepatitis C virus (HCV) entry into hepatocytes is a multistep process that represents promising target for antiviral intervention. The viral envelope protein E1E2 plays critical role in HCV entry. In this study, we sought to identify peptide inhibitors of by screening library overlapping peptides covering E1E2. Screening the identified several novel anti-HCV peptides. Four from glycoprotein E2 were selected further investigation. 50% effective dose (ED50) was approximately 5 nM each peptide....
Baby hamster kidney-21 (BHK-21) cells are widely used to propagate and study many animal viruses using infection transfection techniques. Among various BHK-21 cell clones, the fibroblast-like BHK-21/C-13 line epithelial-like BHK-21/WI-2 commonly clones for alphavirus research. Here we report that were significantly less susceptible primary by chikungunya virus (CHIKV) than cells. The electroporation efficiency of RNA into was also lower BHK-21/C-13. growth CHIKV decreased in compared...
Hepatitis C virus (HCV) entry is mediated by multiple co-receptors including scavenger receptor class B, type I (SR-BI). To elucidate the interactome of human SR-BI, we performed immunoprecipitation experiment coupled with mass spectrometry analysis. UDP-glucose:glycoproteinglucosyltransferase 1 (UGGT1), a key component calnexin cycle involved in protein glycosylation, was identified as SR-BI-interacting protein. Silencing UGGT1 or N-glycosylation inhibitor treatment reduced SR-BI level....
Based on the established two-dimensional asymmetric model of interaction between a nanosecond pulse laser and metallic aluminum, effect beam shaping evaporation ablation dynamics during aluminum by is simulated. The results show that plasma shielding, which has significant influence properties target, occurs mainly in middle phase late pulse. Among three profiles, Gaussian strongest shielding effect. As diameter reshaped flat-top increases, gradually weakens. spatial distribution target...
ABSTRACT The alphavirus chikungunya virus (CHIKV) is a serious human pathogen that can cause large-scale epidemics characterized by fever and joint pain often resulting in chronic arthritis. Infection alphaviruses including CHIKV the closely related Semliki Forest (SFV) induce formation of filopodia-like intercellular long extensions (ILEs). ILEs emanate from an infected cell, stably attach to neighboring mediate cell-to-cell viral transmission resistant neutralizing antibodies. However, our...
The authors declare they have no competing financial interest.
Viral protein 2C plays a critical role in EV-A71 replication. The discovery of binding proteins will likely provide potential targets to treat infection. Here, we global proteomic analysis the human that interact with protein. TRIM4, exportin2, and ARFGAP1 were validated as partners. Further functional studies revealed novel host dependency factors for EV-A71. Moreover, enteroviruses' family interacted exportin2 ARFGAP1. In conclusion, our study provides cellular interactome identifies proviral roles