A5065900243- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Allergic Rhinitis and Sensitization
- Inflammasome and immune disorders
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Immune Cell Function and Interaction
- Pancreatitis Pathology and Treatment
- Heme Oxygenase-1 and Carbon Monoxide
- NF-κB Signaling Pathways
- Diet and metabolism studies
- T-cell and B-cell Immunology
- RNA modifications and cancer
- Immune cells in cancer
- Stress Responses and Cortisol
- S100 Proteins and Annexins
- Air Quality and Health Impacts
- MicroRNA in disease regulation
- Mast cells and histamine
- Redox biology and oxidative stress
- Nutrition and Health in Aging
- Regulation of Appetite and Obesity
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pediatric health and respiratory diseases
- Eosinophilic Esophagitis
University of Vermont
2015-2024
Hospital Civil de Guadalajara
2023
Pulmonary Associates
2014
Abstract IL-1β is a cytokine critical to several inflammatory diseases in which pathogenic Th17 responses are implicated. Activation of the NLRP3 inflammasome by microbial and environmental stimuli can enable caspase-1–dependent processing secretion IL-1β. The acute-phase protein serum amyloid A (SAA) highly induced during responses, wherein it participates systemic modulation innate adaptive immune responses. Elevated levels IL-1β, SAA, IL-17 present subjects with severe allergic asthma,...
Alcohol use disorders are common both in the United States and globally, associated with a variety of co-morbid, inflammation-linked diseases. The pathogenesis many these ailments driven by activation NLRP3 inflammasome, multi-protein intracellular pattern recognition receptor complex that facilitates cleavage secretion pro-inflammatory cytokines IL-1β IL-18. We hypothesized protracted exposure leukocytes to ethanol would amplify inflammasome activation, which help implicate mechanisms...
Nuclear factor (NF)-kappaB is a prominent proinflammatory transcription that plays critical role in allergic airway disease. Previous studies demonstrated inhibition of NF-kappaB epithelium causes attenuation inflammation.We sought to determine if selective activation within the absence other agonists sufficient cause disease.A transgenic mouse expressing doxycycline (Dox)-inducible, constitutively active (CA) version inhibitor kappaB (IkappaB) kinase-beta (IKKbeta) under transcriptional...
Chronic inflammation with mucous metaplasia and airway remodeling are hallmarks of allergic asthma, these outcomes have been associated enhanced expression activation EGFR signaling. Here, we demonstrate ligands such as amphiregulin well constitutive in cultured nasal epithelial cells from asthmatic subjects compared nonasthmatic controls lung tissues mice during house dust mite–induced (HDM-induced) inflammation. was cysteine oxidation within the nonreceptor tyrosine kinase Src, both...
Activation of NF-κB in airway epithelium is observed allergic asthma and induced by inhalation numerous infectious reactive substances. Many the substances that activate are also capable acting as adjuvants to elicit antigen-specific sensitization concomitantly inhaled protein, thereby circumventing inherent bias lung promote tolerance innocuous antigens. We have used a transgenic mouse inducibly expressing constitutively active mutant inhibitor nuclear factor κB (IκB) kinase β ((CA)IKKβ)...
Significance Including sex and gender into preclinical research is central to delineating the mechanisms contributing differences in health disease. The majority of susceptibility infectious diseases, such as influenza A virus (IAV), are believed be due influence hormones on immune system; however, idea that chromosomes also may disease being increasingly recognized. Our finding genetic variation chromosome Y (ChrY) influences survival male mice following IAV infection, associated...
Abstract Flavored e-cigarettes are preferred by the majority of users yet their potential toxicity is unknown. Therefore our aim was to determine effect selected flavored e-cigarettes, with or without nicotine, on allergic airways disease in mice. Balb/c mice were challenged PBS house dust mite (HDM) (Days 0, 7, 14–18) and exposed room air e-cigarette aerosol for 30 min twice daily, 6 days/week from Days 0–18 (n = 8–12/group). Mice Room Air, vehicle control (50%VG/%50PG), Black Licorice,...
Nitrogen dioxide (NO2) is an environmental pollutant and endogenously generated oxidant associated with the development, severity, exacerbation of asthma. NO2 exposure capable allergically sensitizing mice to innocuous inhaled antigen ovalbumin (OVA), promoting neutrophil eosinophil recruitment, a mixed Th2/Th17 response upon challenge that reminiscent severe However, identity IL-17A-producing cells mechanisms governing their ontogeny in NO2-promoted allergic airway disease remain unstudied....
Immunosuppression is a major complication of alcoholism that contributes to increased rates opportunistic infections and sepsis in alcoholics. The NLRP3 inflammasome, multiprotein intracellular pattern recognition receptor complex facilitates the cleavage secretion proinflammatory cytokines IL-1β IL-18, can be inhibited by ethanol, we sought better understand mechanism through which this occurs whether chemically similar molecules exert comparable effects. We show ethanol specifically...
Obese asthma presents with inherent hyperresponsiveness to methacholine or augmented allergen-driven allergic asthma, an even greater magnitude of hyperresponsiveness. These physiologic parameters and accompanying obese symptoms can be reduced by successful weight loss, yet the underlying mechanisms remain incompletely understood. We implemented mouse models diet-induced obesity, dietary surgical environmental allergen exposure examine mediators asthma. report that in these manifests...
Serum amyloid A (SAA) is an apolipoprotein that robustly upregulated in numerous inflammatory diseases and has been implicated as a candidate pro-inflammatory mediator. However, studies comparing endogenous SAAs recombinant forms of the acute phase protein have generated conflicting data on function SAA immunity. We SAA3 knockout mice to evaluate contribution immune-mediated disease, found lacking develop adult-onset obesity metabolic dysfunction along with defects innate immune development....
NLRP3 inflammasome activation induces the cleavage and secretion of IL-1β IL-18, causes pyroptosis. Generated during times energetic crisis (e.g., caloric insufficiency), ketone body β-hydroxybutyrate (BHB) has been reported to inhibit activation. However, conditions under which BHB exerts this activity whether other short-chain carboxylic acids (SCCAs) share effect are unexplored. Since is often produced in high abundance endogenously accompanied by metabolic acidosis, we aimed examine...
Studies comparing endogenous and recombinant serum amyloid A (SAA) have generated conflicting data on the proinflammatory function of these proteins. In exploring this discrepancy, we found that in contrast to commercially sourced human SAA1 (hSAA1) proteins produced
Indoleamine 2,3-dioxygenase (IDO) suppresses the functions of CD4(+) T cells through its ability to metabolize essential amino acid tryptophan. Although activity IDO is required for immunosuppression allergic airway disease by Toll-Like-Receptor 9 (TLR9) agonist, oligonucleotides comprised cytosine and guanine nucleotides linked phosphodiester bonds (CpG) DNA, it unclear whether expression resident lung epithelial sufficient elicit these effects. Therefore, we created a transgenic mouse...
Nitrogen dioxide (NO2) is an air pollutant associated with poor respiratory health, asthma exacerbation, and increased likelihood of inhalational allergies. NO2 also produced endogenously in the lung during acute inflammatory responses. can function as adjuvant, allowing for allergic sensitization to innocuous inhaled antigen generation antigen-specific Th2 immune response manifesting phenotype. As CD11c+ presenting cells are considered critical naïve T cell activation, we investigated role...
Severe, glucocorticoid-resistant asthma comprises 5-7% of patients with asthma. IL-17 is a biomarker severe asthma, and the adoptive transfer Th17 cells in mice sufficient to induce allergic airway disease. Nitrogen dioxide (NO2) an environmental toxin that correlates severity, exacerbation, risk adverse outcomes. Mice are allergically sensitized antigen ovalbumin by exposure NO2 exhibit mixed Th2/Th17 adaptive immune response eosinophil neutrophil recruitment following challenge, phenotype...
Inhalational antigen tolerance typically protects against the development of allergic airway disease but may be overcome to induce sensitization preceding asthma.We examined in vivo whether pre-existing inhalational could by activation transcription factor NF-κB conducting epithelial cells, and used a combination vitro approaches examine mechanisms involved.Wild-type transgenic mice capable expressing constitutively active IκB kinase β (CAIKKβ) epithelium were tolerized inhaled ovalbumin....
Abstract Serum amyloid A (SAA) proteins are a family of acute phase apolipoproteins implicated to directly modulate innate and adaptive immune responses. However, new studies comparing endogenous SAAs recombinant forms these have questioned the function SAA in inflammation immunity. We generated SAA3 knockout mice evaluate contribution lung development immune-mediated disease. While deficiency does not affect generation house dust mite-induced allergic asthma, lacking develop adult-onset...
Many mouse models of allergic asthma exhibit eosinophil-predominant cellularity rather than the mixed-granulocytic cytology in steroid-unresponsive severe disease. Therefore, we sought to implement a novel model antigen-driven, mixed-granulocytic, determine biomarkers disease process and potential therapeutic targets. C57BL/6J wild-type, interleukin-6 knockout (IL-6−/−), IL-6 receptor (IL-6R−/−), mice were injected with an emulsion complete Freund’s adjuvant house dust mite antigen (CFA/HDM)...
Although recognized as an important endocrine organ, little is known about the mechanisms through which adipose tissue can regulate inflammatory responses in distant tissues, such lung that are affected by obesity. To explore potential mechanisms, male C57BL/6J mice were provided either high-fat diet, low-fat or a diet then switched to promote weight loss. Visceral adipocytes cultured vitro generate conditioned media (CM) was used treat both primary (mouse tracheal epithelial cells; MTECs)...