A5037131851- Nitric Oxide and Endothelin Effects
- Iron Metabolism and Disorders
- Photoreceptor and optogenetics research
- Hemoglobin structure and function
- Sulfur Compounds in Biology
- Trace Elements in Health
- Electrochemical sensors and biosensors
- Gene expression and cancer classification
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Fluorescence Microscopy Techniques
- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- bioluminescence and chemiluminescence research
- Hemoglobinopathies and Related Disorders
- Amino Acid Enzymes and Metabolism
- Neonatal Respiratory Health Research
- Analytical Chemistry and Sensors
Sabancı Üniversitesi
2021-2022
Medical University of Graz
2022
Iron is an essential metal for cellular metabolism and signaling, but it has adverse effects in excess. The physiological consequences of iron deficiency are well established, yet the relationship between supplementation pericellular oxygen levels cultured cells their downstream on metalloproteins been less explored. This study exploits metalloprotein geNOps HEK293T epithelial EA.hy926 endothelial to test iron-dependency adapted standard room air (18 kPa O2) or normoxia (5 O2). We show that...
Multispectral live-cell imaging is an informative approach that permits detecting biological processes simultaneously in the spatial and temporal domain by exploiting spectrally distinct biosensors. However, combination of fluorescent biosensors with spectral properties such as different sensitivities, dynamic ranges can undermine accurate co-imaging same analyte subcellular locales. We advanced a single-color multiparametric method, which allows simultaneous detection hydrogen peroxide...
Cellular iron supply is required for various biochemical processes. Measuring bioavailable in cells aids obtaining a better understanding of its activities but technically challenging. Existing techniques have several constraints that make precise localization difficult, and the lack functional readout makes it unclear whether tested labile available metalloproteins. Here, we use geNOps; ferrous iron-dependent genetically encoded fluorescent nitric oxide (NO) biosensor, to measure cellular...
Abstract Iron is an essential metal for cellular metabolism and signaling, but it has adverse effects in excess. The physiological consequences of iron deficiency are well established, yet the relationship between supplementation pericellular oxygen levels cultured cells their downstream on metalloproteins been less explored. This study investigates functionality geNOps - iron-containing metalloprotein NO biosensor HEK293T epithelial EA.hy926 endothelial adapted to standard room air (18 kPa...