A5100612135- Extracellular vesicles in disease
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- RNA Research and Splicing
- Intracerebral and Subarachnoid Hemorrhage Research
- Neurogenesis and neuroplasticity mechanisms
- Caveolin-1 and cellular processes
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Nitric Oxide and Endothelin Effects
- Histone Deacetylase Inhibitors Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Vector-Borne Animal Diseases
- Ferroelectric and Negative Capacitance Devices
- Axon Guidance and Neuronal Signaling
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer Mechanisms and Therapy
- Advanced Glycation End Products research
- Autophagy in Disease and Therapy
- Barrier Structure and Function Studies
- Heat shock proteins research
- Gut microbiota and health
Fudan University
2017-2025
Anhui University of Science and Technology
2025
Xuzhou Medical College
2020-2024
Anhui Provincial Hospital
2024
Lanzhou Institute of Chemical Physics
2024
Chinese Academy of Sciences
2024
Affiliated Hospital of Xuzhou Medical College
2021
Henan Provincial People's Hospital
2021
First Hospital of Jilin University
2020
Jilin University
2020
Plant exosome-like nanoparticles (ELNs) have shown great potential in treating tumor and inflammatory diseases, but the neuroprotective effect of plant ELNs remains unknown. In present study, we isolated characterized novel from Momordica charantia (MC) investigated their effects against cerebral ischemia-reperfusion injury. MC-ELNs were by ultracentrifugation characterized. Male Sprague-Dawley rats subjected to middle artery occlusion (MCAO) MC-ELN injection intravenously. The integrity...
Thoracic radiotherapy patients have higher risks of developing radiation-induced heart disease (RIHD). Ionizing radiation generates excessive reactive oxygens species (ROS) causing oxidative stress, while Momordica. charantia and its extract antioxidant activity. Plant-derived extracellular vesicles (EVs) is emerging as novel therapeutic agent. Therefore, we explored the protective effects -derived EVs-like nanovesicles (MCELNs) against RIHD. Using density gradient centrifugation,...
Blood-brain barrier (BBB) dysfunction causing edema and hemorrhagic transformation is one of the pathophysiological characteristics stroke. Protection BBB integrity has shown great potential in improving stroke outcome. Here, we assessed efficacy exosomes extracted from healthy rat serum protection against ischemic vivo vitro. Exosomes were isolated by gradient centrifugation ultracentrifugation characterized transmission electron microscopy (TEM) nanoparticle tracking video microscope....
Abstract Depression is a common and debilitating condition for which effective treatments are needed. Lepidium meyenii Walp (Maca) plant with potential medicinal effects in treating depression. Recently, there has been growing interest plant‐derived extracellular vesicles (EVs) due to their low toxicity ability transport human cells. Targeting the gut–brain axis, novel strategy depression management, may be achieved through use of Maca‐derived EVs (Maca‐EVs). In this study, we successfully...
Momordica.charantia has antioxidant and neuroprotective effects, but its anti-glioma capacity remains unclear. Plant-derived nanovesicles have shown therapeutic potential in inflammation cancer. This study explored the effects of Momordica.charantia-derived extracellular vesicles-like (MCELNs) treating glioma underlying mechanisms. The MCELNs were successfully isolated characterized similarly to animal cell-derived vesicles. vitro assays identified that inhibited proliferation did not...
Neuronal death resulting from ischemic stroke is the primary cause of adult mortality and disability, effective neuroprotective agents for poststroke intervention are still lacking. Remote postconditioning (RIPostC) has demonstrated significant protective effects against ischemia in various organs; however, specific mechanisms not fully understood. This study investigated potential RIPostC context stroke. Using a rat model middle cerebral artery occlusion, we found that mitigated...
Abstract Artificial synapses are essential components for realizing neuromorphic computing at the physical level. Although numerous artificial synaptic devices have been fabricated in recent years, their performance is often limited by resistance state modulation capabilities and stability. Developing with a high number of intermediate states, excellent linearity, ultralow power consumption remains challenge. This work presents device based on van der Waals layered ionic conductor material,...
Ischemic accumulation of succinate causes cerebral damage by excess production reactive oxygen species. However, it is unknown whether ischemic affects neural stem cell proliferation. In this study, we established a rat model ischemia/reperfusion injury occlusion the middle artery. We found that levels increased in serum and brain tissue (cortex hippocampus) after injury. Oxygen-glucose deprivation reoxygenation stimulated primary cells to produce abundant succinate. Succinate can be...
JOURNAL/nrgr/04.03/01300535-202503000-00029/figure1/v/2024-06-17T092413Z/r/image-tiff It has been shown clinically that continuous removal of ischemia/reperfusion-induced reactive oxygen species is not conducive to the recovery late stroke. Indeed, previous studies have excessive increases in hypochlorous acid after stroke can cause severe damage brain tissue. Our found a small amount still exists later stage stroke, but its specific role and mechanism are currently unclear. To simulate...
As a new expression system, Dunaliella salina (D. salina) has bright prospects and applications in various fields. However, its application is currently restricted because of the low instability foreign gene D. cells. During genetic operation, transformation crucial step for genes system. Although several methods are existing currently, many inherent deficiencies limitations still can be found actual practice. Thus, we attempted to set up rapid method using change salt concentrations salina....
Abstract Aims To explore the function of phosphorylation KAP1 (p-KAP1) at serine-824 site (S824) in proliferation and apoptosis endogenous neural stem cells (NSCs) after cerebral ischemic/reperfusion (I/R). Methods The C17.2 transfected with p-KAP1-expression plasmids expression cell nuclear antigen (PCNA) p-KAP1 were detected by immunofluorescence Western blotting Oxygen Glucose deprivation/reperfusion model (OGD/R). interaction CUL4A PCNA was analyzed immunoprecipitation. In rats MCAO...
RNA polymerase III promoters have been widely used to express short hairpin-RNA (shRNA), microRNA (miRNA), and small guide (sgRNA) in gene functional analysis a variety of organisms including Schistosoma mansoni. However, no endogenous identified japonicum. The lack appropriate S. japonicum has hindered its analysis. Identification is therefore urgent need. Via sequence alignment, 347 bp upstream from the coding region U6 nuclear (snRNA) was identified, cloned, named as (sjU6) promoter. A...
Abstract KRAB domain-associated protein 1 (KAP1) is highly expressed in atherosclerotic plaques. Here, we studied the role of KAP1 atherosclerosis development using a cell model endothelial dysfunction induced by oxidative low-density lipoprotein (OxLDL). The phosphorylation and levels were similar between OxLDL-treated non-treated cells (ECs). depletion significantly inhibited production OxLDL-enhanced reactive oxygen species expression adhesion molecules ECs. Treatment with OxLDL promoted...
The platelets play a crucial role in the progression of multiple medical conditions, such as stroke and tumor metastasis, where antiplatelet therapy may be boon for treating these diseases. In this study, we have attempted to study effects extracted Momordica charantia exosomes (MCEs) on platelet activation, adhesion, aggregation. Adult isolated from healthy individuals were dose-dependently treated with MCEs (0.1, 40, 200 μg/ml). We performed flow cytometry detect expression activation...
Hemorrhagic transformation (HT) and restrictive therapeutic time window are significant limitations of delayed t-PA (tissue-type plasminogen activator) thrombolytic therapy in ischemic stroke patients. Plant Momordica charantia-derived exosome-like nanovesicles (MC-ELNs) can protect the blood–brain barrier (BBB) inhibit neuronal apoptosis rats by inhibiting matrix metalloproteinase 9(MMP-9) expression. This study explored function underlying mechanisms MC-ELNs treating HT. In t-PA-treated...