A5101840593- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Telomeres, Telomerase, and Senescence
- Electrospun Nanofibers in Biomedical Applications
- Hematopoietic Stem Cell Transplantation
- Biomarkers in Disease Mechanisms
- Cancer and Skin Lesions
- Neonatal Respiratory Health Research
- Eosinophilic Disorders and Syndromes
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Pluripotent Stem Cells Research
- Osteoarthritis Treatment and Mechanisms
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Psoriasis: Treatment and Pathogenesis
- Corneal Surgery and Treatments
- Toxin Mechanisms and Immunotoxins
- Nonmelanoma Skin Cancer Studies
- Chemotherapy-related skin toxicity
- Immune cells in cancer
- Skin Diseases and Diabetes
- Pancreatic function and diabetes
- Cutaneous lymphoproliferative disorders research
University of Ulsan
2020-2024
Asan Medical Center
2024
Ulsan College
2020-2024
Medipost (South Korea)
2012-2022
Wonkwang University
2012
Various source-derived mesenchymal stem cells (MSCs) have been considered for cell therapeutics in incurable diseases. To characterize MSCs from different sources, we compared human bone marrow (BM), adipose tissue (AT), and umbilical cord blood-derived (UCB-MSCs) surface antigen expression, differentiation ability, proliferation capacity, clonality, tolerance aging, paracrine activity. Although tissues similar levels of immunosuppressive activity, UCB-MSCs had the highest rate significantly...
Aims: Cellular senescence and its secretory phenotype (senescence-associated [SASP]) develop after long-term expansion of mesenchymal stromal cells (MSCs). Further investigation this is required to improve the therapeutic efficacy MSC-based cell therapies. In study, we show that positive feedback between SASP inherent processes plays a crucial role in umbilical cord blood-derived MSCs (UCB-MSCs). Results: We found monocyte chemoattractant protein-1 (MCP-1) was secreted as dominant component...
Mesenchymal stem cells (MSCs) are of particular interest for the treatment immune-related diseases due to their immunosuppressive capacity. Here, we show that Small MSCs primed with Hypoxia and Calcium ions (SHC-MSCs) exhibit enhanced stemness immunomodulatory functions treating allogeneic conflicts. Compared naïve cultured human umbilical cord blood-derived MSCs, SHC-MSCs were resistant passage-dependent senescence mediated via monocyte chemoattractant protein-1 p53/p21 cascade secreted...
Abstract Therapeutic applications of mesenchymal stem cells (MSCs) for treating various diseases have increased in recent years. To ensure that treatment is effective, an adequate MSC dosage should be determined before these are used therapeutic purposes. obtain a sufficient number applications, MSCs must expanded long-term cell culture, which inevitably triggers cellular senescence. In this study, we investigated the surface markers human umbilical cord blood-derived (hUCB-MSCs) associated...
Bronchopulmonary dysplasia (BPD), caused by hyperoxia in newborns and infants, results lung damage abnormal pulmonary function. However, the current treatments for BPD are steroidal pharmacological therapies, which cause neurodevelopmental impairment. Treatment with umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) is an efficient alternative approach. To prevent inflammation BPD, this study investigated hypothesis that a key regulator was secreted MSCs to polarize inflammatory...
Mesenchymal stem cells (MSCs) represent a promising means to promote tissue regeneration. However, the heterogeneity of MSCs impedes their use for regenerative medicine. Further investigation this phenotype is required develop cell therapies with improved clinical efficacy. Here, small-sized population human umbilical cord blood-derived (UCB-MSCs) was isolated using filter and centrifuge system analyze its characteristics. Consequently, showed higher growth lower senescence. Additionally, it...
Psoriasis is an inflammatory skin disease characterized by the hyperproliferative epidermal keratinocytes and significant immune cells infiltration, leading to cytokines production such as IL-1β, TNF-α, IL-23, IL-17. Recent study highlights critical role of IL-1β in induction activation pathogenic Th17 IL-17-producing γδ T cells, contributing psoriasis. However, mechanism underlying dysregulation psoriasis pathogenesis unclear. Autophagy regulates has a pleiotropic effect on disorders....
Therapeutic treatment of various inflammation-related diseases using mesenchymal stem cells (MSCs) has increased in recent years because the paracrine action these but shows several limitations. First, MSC-based therapies exhibit varying efficacies; thus, biomarkers should be determined to identify who may benefit from candidate therapeutic agents. Second, mechanism underlying effects is poorly understood. To evaluate human umbilical cord blood-derived MSCs (UCB-MSCs) on macrophages,...
Mesenchymal stem cells- (MSCs-) based therapies show different degrees of efficacies for the treatment various diseases, including lipogenesis. We evaluated adipogenic differentiation ability human umbilical cord blood-derived MSCs (hUCB-MSCs) from donors and examined contribution intracellular calcium (Ca 2+ ) level to this diversity. hUCB-MSCs treated with Ca or chelator BAPTA-AM increased decreased differentiation, respectively. Canonical Wnt5a/ β -catenin expression during hUCB-MSCs....
In order to provide a sufficient number of cells for clinical use, mesenchymal stem (MSCs) must be cultured long-term expansion, which inevitably triggers cellular senescence. Although the small size MSCs is known as critical determinant their fate, main regulators cell senescence and underlying signaling have not been addressed. Umbilical cord blood-derived (UCB-MSCs) were obtained using size-isolation methods then with control or investigate major factors that modulate MSC Cytokine array...
Osteoblasts, which are derived from pluripotent mesenchymal stem cells (MSCs), play an important role in hematopoiesis. Human parathyroid hormone (hPTH) induces osteoblasts to produce many factors that essential hematopoietic cells. However, little is known about the impact of hPTH on MSCs enhance We determined optimal dose was necessary vitro for increased osteoblast function. In addition, we compared MSC and function explore The mRNA expression levels granulocyte colony-stimulating factor...
Osteoarthritis (OA), being the most prominent degenerative joint disease is affecting millions of elderly people worldwide. Although Andrographis paniculata an ethnic medicine with a long history used as analgesic agent, no study using monosodium iodoacetate (MIA) model has investigated its potential activities against OA. In this study, experimental OA was induced in rats knee injection MIA, which represents pathological characteristics humans. A. extract (APE) substantially reversed loss...
Autophagy plays a critical role in stem cell maintenance and is related to growth cellular senescence. It important find quality-control marker for predicting senescent cells. This study verified that CD47 could be candidate select efficient mesenchymal cells (MSCs) enhance the therapeutic effects of therapy by analyzing antibody surface array. expression was significantly decreased during expansion MSCs vitro ( <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"> <a:mi>p</a:mi>...
Mesenchymal stem cells (MSCs) are accessible, abundantly available, and capable of regenerating; they have the potential to be developed as therapeutic agents for diseases. However, concerns remain in their further application. In this study, we a SMall cell+Ultra Potent+Scale UP cell (SMUP-Cell) platform improve whole-cell processing, including manufacturing bioreactors xeno-free solutions commercialization. To confirm superiority SMUP-Cell improvements, demonstrated that molecule secreted...
Mesenchymal stem cells (MSCs) are recognized as potential treatments for multiple degenerative and inflammatory disorders a number of animal human studies have indicated their therapeutic effects. There also several clinically approved medicinal products that manufactured using these cells. For such large-scale manufacturing requirements, the in vitro expansion harvested MSCs is essential. Multiple subculturing MSCs, however, provokes cellular senescence processes which known to deteriorate...
The BCR-ABL1 fusion gene generating an oncogenic tyrosine kinase is a hallmark of chronic myeloid leukemia (CML), which can be successfully targeted by inhibitors (TKIs). However, treatment-free remission has been achieved in minority patients due to evolving TKI resistance and intolerance. Primary or acquired the approved TKIs progression blast crisis (BC), thus, remain major clinical challenge that requires alternative therapeutic strategies. Here, we first demonstrate donor natural killer...
Purpose:We evaluated the effect of human parathyroid hormone (hPTH) on engraftment and/or in vivo expansion hematopoietic stem cells an umbilical cord blood (UCB)-xenotransplantation model.In addition, we assessed its expression cell adhesion molecules.Materials and Methods: Female NOD/ SCID mice received sublethal total body irradiation with a single dose 250 cGy.Eighteen to 24 hours after irradiation, 1×10 7 UCB-derived mononuclear (MNCs) 5×10 6 mesenchymal (MSCs) were infused via tail...
Mesenchymal stem cells (MSCs) have the ability to self-renew and differentiate into multi-lineage cells, thus highlighting feasibility of using umbilical cord blood-derived MSCs (UCB-MSCs) for cell-therapy tissue-engineering. However, low numbers UCB-MSC derived from clinical samples requires that an ex vivo expansion step be implemented. As most reside in oxygen tension environments (i.e., hypoxia), we cultured UCB-MSCs under 3% O2 or 21% following parameters were examined: proliferation,...
Mesenchymal stem cells (MSCs) are one of the most extensively studied cell types owing to their capacity for differentiation into multiple lineages as well ability secrete regenerative factors and modulate immune functions. However, issues remain regarding further application therapy. Here, demonstrate superiority improvement MSCs, we divided umbilical cord blood-derived MSCs (UCB-MSCs) from 15 donors two groups based on efficacy revealed donor-dependent variations in anti-inflammatory...